Detailed information NTG1031405

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: AB3K28_RS04500
• Genome accession: NZ_CP165737
• Coordinates: 928082..929062 (+)
• Length: 326 a.a.
• NCBI ID: WP_001326494.1
• Uniprot ID: D3GUL4
• Organism: Escherichia coli strain PEC1013
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 923082..934062

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB3K28_RS04470	-	923460..924446 (-)	987	WP_000784004.1	TRAP transporter substrate-binding protein	-
AB3K28_RS04475	hemW	924759..925895 (-)	1137	WP_000239986.1	radical SAM family heme chaperone HemW	-
AB3K28_RS04480	rdgB	925888..926481 (-)	594	WP_001174754.1	XTP/dITP diphosphatase	-
AB3K28_RS04485	yggU	926489..926779 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
AB3K28_RS04490	yggT	926776..927342 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
AB3K28_RS04495	yggS	927360..928064 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
AB3K28_RS04500	pilT	928082..929062 (+)	981	WP_001326494.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB3K28_RS04505	ruvX	929246..929662 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
AB3K28_RS04510	yqgE	929662..930225 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
AB3K28_RS04515	gshB	930334..931284 (-)	951	WP_000593273.1	glutathione synthase	-
AB3K28_RS04520	rsmE	931297..932028 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
AB3K28_RS04525	endA	932108..932815 (-)	708	WP_000286500.1	deoxyribonuclease I	-
AB3K28_RS04530	yggI	932910..933407 (-)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35994.16 Da
• Isoelectric Point: 6.0804

>NTDB_id=1031405 AB3K28_RS04500 WP_001326494.1 928082..929062(+) (pilT) [Escherichia coli strain PEC1013]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1031405 AB3K28_RS04500 WP_001326494.1 928082..929062(+) (pilT) [Escherichia coli strain PEC1013]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (61-239)

3D structure

• AlphaFold DB: D3GUL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	49.235	100	0.494
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.235	100	0.494
pilT	Neisseria meningitidis 8013	48.476	100	0.488
pilT	Neisseria gonorrhoeae MS11	48.171	100	0.485
pilT	Acinetobacter baumannii D1279779	46.483	100	0.466
pilT	Acinetobacter baylyi ADP1	46.483	100	0.466
pilT	Acinetobacter nosocomialis M2	46.483	100	0.466
pilT	Acinetobacter baumannii strain A118	46.483	100	0.466
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain ERS1305867	45.912	97.546	0.448
pilT	Legionella pneumophila strain Lp02	45.912	97.546	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	40.373	98.773	0.399
pilU	Pseudomonas stutzeri DSM 10701	38.554	100	0.393
pilU	Acinetobacter baylyi ADP1	36.061	100	0.365
pilB	Legionella pneumophila strain ERS1305867	30.89	100	0.362