Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB8S08_RS08925 Genome accession   NZ_CP165718
Coordinates   1876348..1877382 (-) Length   344 a.a.
NCBI ID   WP_110076395.1    Uniprot ID   A0AB39X806
Organism   Pseudidiomarina sp. PP-1MA     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1871348..1882382
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8S08_RS08895 (AB8S08_08895) - 1871703..1872497 (-) 795 WP_369742540.1 undecaprenyl-diphosphate phosphatase -
  AB8S08_RS08900 (AB8S08_08900) folK 1872522..1873070 (-) 549 WP_369742541.1 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase -
  AB8S08_RS08905 (AB8S08_08905) folB 1873067..1873420 (-) 354 WP_110076391.1 dihydroneopterin aldolase -
  AB8S08_RS08910 (AB8S08_08910) plsY 1873517..1874149 (+) 633 WP_110076392.1 glycerol-3-phosphate 1-O-acyltransferase PlsY -
  AB8S08_RS08915 (AB8S08_08915) tsaD 1874150..1875169 (-) 1020 WP_369742542.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  AB8S08_RS08920 (AB8S08_08920) pilU 1875233..1876348 (-) 1116 WP_369742543.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8S08_RS08925 (AB8S08_08925) pilT 1876348..1877382 (-) 1035 WP_110076395.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8S08_RS08930 (AB8S08_08930) - 1877417..1878103 (+) 687 WP_110076421.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8S08_RS08935 (AB8S08_08935) proC 1878127..1878954 (+) 828 WP_110076396.1 pyrroline-5-carboxylate reductase -
  AB8S08_RS08940 (AB8S08_08940) - 1879017..1879553 (+) 537 WP_369742544.1 YggT family protein -
  AB8S08_RS08945 (AB8S08_08945) - 1879596..1880072 (+) 477 WP_313933077.1 DUF4426 domain-containing protein -
  AB8S08_RS08950 (AB8S08_08950) - 1880079..1881806 (-) 1728 WP_369742545.1 potassium/proton antiporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38269.73 Da        Isoelectric Point: 7.1596

>NTDB_id=1031329 AB8S08_RS08925 WP_110076395.1 1876348..1877382(-) (pilT) [Pseudidiomarina sp. PP-1MA]
MDITELLSFAVKHKSSDLHLSAESPPMIRVDGDVRKINIPALSHKQVQELVYDIMNDRQRKEYEQNLECDFSFEVPNLAR
FRVNAFVQNRGAGAVFRTIPSEVLTLEQLGAPEIFKTISDNPRGLVLVTGPTGSGKSTTLAAMVDYINENKFHHILTIED
PIEFVHKNKRSLVNQREVHRDTHSFNNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
VDVFPGDEKPMIRSMLSESLRAVISQTLLKKVGGGRVAAHEIMIATSAIKNLIREDKIAQMYSSIQTGAAHGMQTLDQSL
QNLLNQGTVTRADAKAKANHKDAF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1031329 AB8S08_RS08925 WP_110076395.1 1876348..1877382(-) (pilT) [Pseudidiomarina sp. PP-1MA]
ATGGATATCACTGAGTTACTCAGCTTTGCTGTTAAACATAAATCGTCAGACTTACATTTGTCAGCAGAGTCACCTCCTAT
GATACGGGTAGATGGTGACGTTCGTAAAATAAATATCCCGGCCTTATCGCACAAGCAAGTGCAAGAGCTGGTGTATGACA
TTATGAACGACCGCCAGCGTAAAGAGTACGAACAGAATCTAGAATGTGATTTCTCGTTTGAGGTACCGAATCTGGCGCGC
TTTCGGGTTAACGCATTTGTACAAAACCGTGGTGCTGGCGCCGTCTTTCGTACTATTCCTAGCGAGGTTCTTACACTGGA
ACAACTGGGTGCTCCAGAGATCTTTAAAACGATCTCCGATAACCCTCGTGGACTCGTGTTAGTGACTGGGCCCACCGGTT
CAGGTAAGTCTACGACGCTGGCGGCTATGGTGGATTACATTAATGAGAATAAATTTCATCACATTTTGACCATCGAAGAT
CCCATCGAGTTTGTTCATAAGAACAAACGTAGCTTGGTTAACCAACGTGAAGTGCATCGCGACACCCACAGTTTTAACAA
CGCATTACGCTCGGCCCTGCGTGAAGACCCTGATGTGATTTTAGTGGGTGAGATGCGTGATTTAGAAACCATTCGGTTAG
CCATGACAGCAGCTGAAACTGGACACTTAGTGTTTGGTACCTTGCACACAACCTCTGCCCCAAAAACCATTGACCGGATT
GTCGATGTGTTTCCGGGCGATGAGAAACCTATGATTCGCTCCATGTTATCTGAATCACTGCGCGCCGTGATTTCGCAAAC
CTTGCTGAAAAAGGTGGGTGGGGGGCGAGTTGCCGCTCATGAGATCATGATTGCGACGTCTGCCATTAAGAACTTAATTC
GCGAAGATAAAATTGCACAGATGTATTCGTCCATTCAAACGGGTGCTGCCCATGGCATGCAAACGCTTGACCAATCTCTG
CAAAACTTACTCAACCAAGGCACTGTAACCCGAGCTGACGCTAAAGCTAAAGCTAATCACAAGGATGCCTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

76.453

100

0.765
  pilT Acinetobacter nosocomialis M2

75.581

100

0.756
  pilT Acinetobacter baumannii D1279779

75.581

100

0.756
  pilT Acinetobacter baumannii strain A118

75.581

100

0.756
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Acinetobacter baylyi ADP1

75

100

0.75
  pilT Legionella pneumophila strain Lp02

72.674

100

0.727
  pilT Legionella pneumophila strain ERS1305867

72.674

100

0.727
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.513

97.965

0.701
  pilT Vibrio cholerae strain A1552

71.513

97.965

0.701
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.776

96.221

0.517
  pilU Pseudomonas stutzeri DSM 10701

41.765

98.837

0.413
  pilU Vibrio cholerae strain A1552

41.471

98.837

0.41
  pilU Acinetobacter baylyi ADP1

38.71

99.128

0.384

Multiple sequence alignment