Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB8S08_RS08920 Genome accession   NZ_CP165718
Coordinates   1875233..1876348 (-) Length   371 a.a.
NCBI ID   WP_369742543.1    Uniprot ID   A0AB39X704
Organism   Pseudidiomarina sp. PP-1MA     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1870233..1881348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8S08_RS08890 (AB8S08_08890) - 1870575..1871690 (+) 1116 WP_369742539.1 CCA tRNA nucleotidyltransferase -
  AB8S08_RS08895 (AB8S08_08895) - 1871703..1872497 (-) 795 WP_369742540.1 undecaprenyl-diphosphate phosphatase -
  AB8S08_RS08900 (AB8S08_08900) folK 1872522..1873070 (-) 549 WP_369742541.1 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase -
  AB8S08_RS08905 (AB8S08_08905) folB 1873067..1873420 (-) 354 WP_110076391.1 dihydroneopterin aldolase -
  AB8S08_RS08910 (AB8S08_08910) plsY 1873517..1874149 (+) 633 WP_110076392.1 glycerol-3-phosphate 1-O-acyltransferase PlsY -
  AB8S08_RS08915 (AB8S08_08915) tsaD 1874150..1875169 (-) 1020 WP_369742542.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  AB8S08_RS08920 (AB8S08_08920) pilU 1875233..1876348 (-) 1116 WP_369742543.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8S08_RS08925 (AB8S08_08925) pilT 1876348..1877382 (-) 1035 WP_110076395.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8S08_RS08930 (AB8S08_08930) - 1877417..1878103 (+) 687 WP_110076421.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8S08_RS08935 (AB8S08_08935) proC 1878127..1878954 (+) 828 WP_110076396.1 pyrroline-5-carboxylate reductase -
  AB8S08_RS08940 (AB8S08_08940) - 1879017..1879553 (+) 537 WP_369742544.1 YggT family protein -
  AB8S08_RS08945 (AB8S08_08945) - 1879596..1880072 (+) 477 WP_313933077.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40891.98 Da        Isoelectric Point: 6.2886

>NTDB_id=1031328 AB8S08_RS08920 WP_369742543.1 1875233..1876348(-) (pilU) [Pseudidiomarina sp. PP-1MA]
MSVLDKLLKLMVERQSSDLFISAGLAPSAKINGELQSLAEQKLSDNAALAIVKEALGDDHLEQFITHKEANFAIFREGIG
RFRVSAFWQRQKAGMVVRRIVTEIPTVEQLKLPSILTQLIMSKRGLLLVVGGTGTGKSTSLAALIGYRNQHSKGHILTIE
DPVEFVHDHAQCMVTQREVGQDTESFEAALKSSLRQAPDVILVGEIRSQETMEYALTFAETGHLCVATLHANNANQAIER
IMQLVPKEKIHKLMFDLSLNLRGIVAQQLVPTTDGGRTAAIEILLNSPIIAEKIATGDMGELKEIMARSRELGMQTFDQA
LYDLYKAGRITYDNAMRHADAPNDLRLRIKLSGENHGDDDSSLKGVTVDLD

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=1031328 AB8S08_RS08920 WP_369742543.1 1875233..1876348(-) (pilU) [Pseudidiomarina sp. PP-1MA]
ATGTCGGTGTTGGATAAGCTCTTAAAACTCATGGTGGAACGACAAAGTTCAGATTTGTTTATTTCGGCGGGGTTAGCTCC
TAGTGCCAAAATTAATGGTGAGCTGCAGTCGTTAGCAGAACAGAAGTTGAGTGATAATGCGGCCTTAGCCATCGTCAAAG
AAGCTCTTGGGGATGATCATTTAGAGCAATTTATTACCCATAAAGAAGCCAATTTCGCGATTTTCCGCGAAGGCATTGGC
CGGTTTCGGGTGAGTGCATTCTGGCAACGCCAAAAAGCCGGTATGGTAGTACGCCGTATTGTTACCGAGATACCTACAGT
AGAGCAGCTTAAACTTCCTAGTATTCTAACCCAACTCATTATGAGTAAGCGCGGTTTGTTGCTAGTGGTAGGGGGCACAG
GTACCGGTAAGTCAACCAGTTTGGCGGCTTTGATTGGCTACCGCAACCAGCATTCAAAGGGGCATATTCTGACCATTGAA
GACCCCGTCGAATTTGTGCATGACCACGCTCAATGCATGGTCACACAGCGTGAGGTAGGACAGGATACAGAATCGTTTGA
GGCAGCGTTAAAAAGCTCGTTACGGCAAGCTCCCGATGTGATTTTAGTTGGGGAGATACGTTCTCAAGAGACTATGGAAT
ACGCTTTAACCTTTGCTGAAACAGGTCATTTGTGTGTGGCTACGTTGCACGCTAACAATGCCAACCAAGCGATAGAGCGG
ATCATGCAACTGGTGCCGAAAGAGAAGATTCACAAGCTCATGTTTGATTTATCGCTCAACCTACGTGGTATTGTGGCGCA
ACAGTTAGTTCCCACTACTGATGGGGGGCGTACTGCAGCCATTGAAATTCTACTCAATAGCCCGATCATTGCGGAGAAGA
TAGCCACCGGTGACATGGGCGAGTTAAAAGAAATCATGGCGCGTTCGCGTGAATTAGGCATGCAAACTTTTGACCAAGCT
CTTTATGATCTCTACAAGGCGGGCCGTATTACATACGATAATGCGATGCGTCATGCAGATGCACCAAATGACTTACGCTT
GCGGATCAAACTCAGTGGTGAAAATCACGGTGATGATGACAGTTCATTGAAGGGTGTTACCGTTGATCTAGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

57.995

99.461

0.577
  pilU Vibrio cholerae strain A1552

55.946

99.73

0.558
  pilU Acinetobacter baylyi ADP1

56.941

95.148

0.542
  pilT Legionella pneumophila strain Lp02

41.212

88.949

0.367
  pilT Legionella pneumophila strain ERS1305867

41.212

88.949

0.367
  pilT Vibrio cholerae strain A1552

41.796

87.062

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.796

87.062

0.364
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.706

91.644

0.364

Multiple sequence alignment