Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AACH48_RS18320 Genome accession   NZ_AP027942
Coordinates   4027821..4028855 (+) Length   344 a.a.
NCBI ID   WP_005305010.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain VA84     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4022821..4033855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH48_RS18290 (VAWG005_35420) - 4024491..4024802 (+) 312 WP_005304992.1 hypothetical protein -
  AACH48_RS18295 (VAWG005_35430) - 4024866..4025285 (-) 420 WP_005304995.1 DUF4426 domain-containing protein -
  AACH48_RS18300 (VAWG005_35440) yggU 4025304..4025603 (-) 300 WP_005304998.1 DUF167 family protein YggU -
  AACH48_RS18305 (VAWG005_35450) - 4025603..4026154 (-) 552 WP_017780458.1 YggT family protein -
  AACH48_RS18310 (VAWG005_35460) proC 4026195..4027019 (-) 825 WP_319909716.1 pyrroline-5-carboxylate reductase -
  AACH48_RS18315 (VAWG005_35470) - 4027080..4027781 (-) 702 WP_019838903.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACH48_RS18320 (VAWG005_35480) pilT 4027821..4028855 (+) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  AACH48_RS18325 (VAWG005_35490) pilU 4028884..4029993 (+) 1110 WP_019838905.1 type IVa pilus ATPase TapU Machinery gene
  AACH48_RS18330 (VAWG005_35500) yaaA 4030025..4030798 (+) 774 WP_019838906.1 peroxide stress protein YaaA -
  AACH48_RS18335 (VAWG005_35510) srmB 4030975..4032198 (-) 1224 WP_019838907.1 ATP-dependent RNA helicase SrmB -
  AACH48_RS18340 (VAWG005_35520) - 4032327..4033037 (+) 711 WP_005305026.1 methyltransferase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38319.12 Da        Isoelectric Point: 6.7439

>NTDB_id=103059 AACH48_RS18320 WP_005305010.1 4027821..4028855(+) (pilT) [Aeromonas dhakensis strain VA84]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEYPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGMVAALDAKAKAVDPNTI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=103059 AACH48_RS18320 WP_005305010.1 4027821..4028855(+) (pilT) [Aeromonas dhakensis strain VA84]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAACATAAAGCCTCGGATCTACACCTCTCAGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGCGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAGCACCGGGAAGTACACGCCCTCATCTATGACA
TCATGAACGACCACCAGCGCAAGGAACTGGAAGAGAACTTCGAGGTGGACTTCTCGTTCGAGGTGCCGAACCTGGCCCGC
TTCCGGGTCAACGCCTTCCAACAGGCGCGCGGCTCCGGCGCGGTATTTCGGACCATCCCCAGCACTGTGCTGAGCCTCGA
AGATCTGGACGCCCCGGAGATCTTTCGCAAGATTGCCGAATACCCCCGCGGCCTGGTGCTGGTGACCGGACCGACCGGCT
CGGGCAAGTCCACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTTCATCACCACATCCTCACCATCGAGGAT
CCCATCGAATTCGTGCACGAGAACAAGCGCTGTCTAGTGAACCAGCGGGAAGTGCACCGCGATACCAAGAGCTTCAGCAA
CGCCCTGCGTTCGGCGCTGCGGGAAGACCCGGACATCATACTGGTGGGTGAAATGCGCGACCTCGAGACCATTCGCCTGG
CCATGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATAGACCGGATC
ATCGACGTCTTCCCCGGTGCGGAAAAAGACATGGTTCGCTCCATGCTCTCCGAATCGTTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATCGGTGGCGGCCGGGTGGCGGCCCACGAAATCATGATGGGGATCCCCGCCGTGCGTAACCTCATCC
GGGAGGACAAGATCGCCCAGCTCTATTCGGTGATCCAAACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGCCGCGGCATGGTGGCGGCGCTGGATGCCAAGGCCAAGGCGGTCGATCCCAACACCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Acinetobacter nosocomialis M2

77.035

100

0.77
  pilT Acinetobacter baumannii D1279779

76.744

100

0.767
  pilT Acinetobacter baumannii strain A118

76.744

100

0.767
  pilT Pseudomonas stutzeri DSM 10701

76.833

99.128

0.762
  pilT Pseudomonas aeruginosa PAK

75.953

99.128

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

76.261

97.965

0.747
  pilT Vibrio cholerae strain A1552

76.261

97.965

0.747
  pilT Legionella pneumophila strain Lp02

72.647

98.837

0.718
  pilT Legionella pneumophila strain ERS1305867

72.647

98.837

0.718
  pilT Neisseria meningitidis 8013

70.796

98.547

0.698
  pilT Neisseria gonorrhoeae MS11

70.501

98.547

0.695
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407

Multiple sequence alignment