Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AACH48_RS18325 Genome accession   NZ_AP027942
Coordinates   4028884..4029993 (+) Length   369 a.a.
NCBI ID   WP_019838905.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain VA84     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4023884..4034993
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH48_RS18290 (VAWG005_35420) - 4024491..4024802 (+) 312 WP_005304992.1 hypothetical protein -
  AACH48_RS18295 (VAWG005_35430) - 4024866..4025285 (-) 420 WP_005304995.1 DUF4426 domain-containing protein -
  AACH48_RS18300 (VAWG005_35440) yggU 4025304..4025603 (-) 300 WP_005304998.1 DUF167 family protein YggU -
  AACH48_RS18305 (VAWG005_35450) - 4025603..4026154 (-) 552 WP_017780458.1 YggT family protein -
  AACH48_RS18310 (VAWG005_35460) proC 4026195..4027019 (-) 825 WP_319909716.1 pyrroline-5-carboxylate reductase -
  AACH48_RS18315 (VAWG005_35470) - 4027080..4027781 (-) 702 WP_019838903.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACH48_RS18320 (VAWG005_35480) pilT 4027821..4028855 (+) 1035 WP_005305010.1 type IVa pilus ATPase TapT Machinery gene
  AACH48_RS18325 (VAWG005_35490) pilU 4028884..4029993 (+) 1110 WP_019838905.1 type IVa pilus ATPase TapU Machinery gene
  AACH48_RS18330 (VAWG005_35500) yaaA 4030025..4030798 (+) 774 WP_019838906.1 peroxide stress protein YaaA -
  AACH48_RS18335 (VAWG005_35510) srmB 4030975..4032198 (-) 1224 WP_019838907.1 ATP-dependent RNA helicase SrmB -
  AACH48_RS18340 (VAWG005_35520) - 4032327..4033037 (+) 711 WP_005305026.1 methyltransferase -
  AACH48_RS18345 (VAWG005_35530) brnQ 4033229..4034536 (+) 1308 WP_139430691.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41139.26 Da        Isoelectric Point: 6.4437

>NTDB_id=103060 AACH48_RS18325 WP_019838905.1 4028884..4029993(+) (pilU) [Aeromonas dhakensis strain VA84]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSPEHFERYLRTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSPDGRRRFAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGSGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=103060 AACH48_RS18325 WP_019838905.1 4028884..4029993(+) (pilU) [Aeromonas dhakensis strain VA84]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCGCCCGTAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCATCTGCAGTCGCTGGCAGAAGCCCCCCTCGACAAGGCAAGGGCGCTGGCGTTGGTCAAGGAGA
CCCTGAGCCCGGAGCACTTCGAACGCTACCTGCGCACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTACTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATATTGCAGGAGATAGCCCTGGCCAAGCGCGGACTGGTACTGTTCGTCGGTGCCACCGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGAAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACGGAGTCGTTCGACGT
GGCGCTGAAGAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAATTTGCCGAGACCGGTCACCTCTGCCTCGCCACCCTGCATGCCAACAACGCCAACCAGGCGCTTGATCGCATC
CTGCACCTGGTGCCCCAGGAGAAACACCGTCAGTTCCTGTTTGATCTCTCCTTCAATCTGCGCGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCCGGATGGACGGCGTCGTTTTGCCGCCTTCGAGGTGTTGCTCAACACCCCGCTCATCACCGACATCA
TCCGCAAGGGGGAGATGCACCGCCTCAAGGAGGTGATGACCAAGTCCACCGAACTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCCGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGCCGAGAACAGCTGGGTTCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

61.141

99.729

0.61
  pilU Pseudomonas stutzeri DSM 10701

59.829

95.122

0.569
  pilU Acinetobacter baylyi ADP1

56.941

95.664

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363

Multiple sequence alignment