Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AACH65_RS18365 Genome accession   NZ_AP027937
Coordinates   3967723..3968757 (-) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain VA60     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3962723..3973757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH65_RS18335 (VAWG004_36210) glnK 3963304..3963642 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  AACH65_RS18340 (VAWG004_36220) arfB 3963844..3964260 (+) 417 WP_019445599.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  AACH65_RS18345 (VAWG004_36230) - 3964293..3964784 (+) 492 WP_338710485.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  AACH65_RS18350 (VAWG004_36240) - 3965144..3965569 (-) 426 WP_005340360.1 hypothetical protein -
  AACH65_RS18355 (VAWG004_36250) yaaA 3965764..3966537 (-) 774 WP_005340358.1 peroxide stress protein YaaA -
  AACH65_RS18360 (VAWG004_36260) pilU 3966585..3967694 (-) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  AACH65_RS18365 (VAWG004_36270) pilT 3967723..3968757 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  AACH65_RS18370 (VAWG004_36280) - 3968797..3969498 (+) 702 WP_088870178.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACH65_RS18375 (VAWG004_36290) proC 3969641..3970465 (+) 825 WP_088870179.1 pyrroline-5-carboxylate reductase -
  AACH65_RS18380 (VAWG004_36300) - 3970477..3971028 (+) 552 WP_047436998.1 YggT family protein -
  AACH65_RS18385 (VAWG004_36310) yggU 3971028..3971330 (+) 303 WP_088870180.1 DUF167 family protein YggU -
  AACH65_RS18390 (VAWG004_36320) - 3971374..3971793 (+) 420 WP_005357936.1 DUF4426 domain-containing protein -
  AACH65_RS18395 (VAWG004_36330) - 3971895..3973091 (-) 1197 WP_005348874.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=102980 AACH65_RS18365 WP_005340354.1 3967723..3968757(-) (pilT) [Aeromonas veronii strain VA60]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=102980 AACH65_RS18365 WP_005340354.1 3967723..3968757(-) (pilT) [Aeromonas veronii strain VA60]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTAGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTACCCAATCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCACGCGGCTCGGGCGCGGTATTTCGTACCATCCCCAGCACGGTGCTGAGCCTCGA
GGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGGGGTCTGGTGTTGGTGACCGGCCCGACCGGTT
CCGGTAAGTCGACCACCCTGGCGGCCATGGTCAACTACATCAACGAGAACTTCCATCACCATATCCTCACCATCGAAGAC
CCCATCGAATTCGTCCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTGCACCGCGATACCAAGAGCTTCAGCAA
CGCCCTACGCTCGGCCCTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAAATGCGCGACCTTGAGACCATTCGTCTGG
CCATGACCGCAGCTGAAACTGGCCATCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGTGCGGAGAAGGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTCAAGCGCATTGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATGGGCATTCCGGCGGTGCGTAACCTCATCC
GGGAGGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGCCTC
AAGCAGCTGGTGAGTCGTGGCGTAGTAGCATCCCTCGATGCCAAGGCCAAGGCCGTTGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407

Multiple sequence alignment