Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AACH65_RS18360 Genome accession   NZ_AP027937
Coordinates   3966585..3967694 (-) Length   369 a.a.
NCBI ID   WP_005340356.1    Uniprot ID   -
Organism   Aeromonas veronii strain VA60     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3961585..3972694
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH65_RS18330 (VAWG004_36200) - 3962039..3963046 (-) 1008 WP_127007060.1 Fe(3+) ABC transporter substrate-binding protein -
  AACH65_RS18335 (VAWG004_36210) glnK 3963304..3963642 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  AACH65_RS18340 (VAWG004_36220) arfB 3963844..3964260 (+) 417 WP_019445599.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  AACH65_RS18345 (VAWG004_36230) - 3964293..3964784 (+) 492 WP_338710485.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  AACH65_RS18350 (VAWG004_36240) - 3965144..3965569 (-) 426 WP_005340360.1 hypothetical protein -
  AACH65_RS18355 (VAWG004_36250) yaaA 3965764..3966537 (-) 774 WP_005340358.1 peroxide stress protein YaaA -
  AACH65_RS18360 (VAWG004_36260) pilU 3966585..3967694 (-) 1110 WP_005340356.1 type IVa pilus ATPase TapU Machinery gene
  AACH65_RS18365 (VAWG004_36270) pilT 3967723..3968757 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  AACH65_RS18370 (VAWG004_36280) - 3968797..3969498 (+) 702 WP_088870178.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACH65_RS18375 (VAWG004_36290) proC 3969641..3970465 (+) 825 WP_088870179.1 pyrroline-5-carboxylate reductase -
  AACH65_RS18380 (VAWG004_36300) - 3970477..3971028 (+) 552 WP_047436998.1 YggT family protein -
  AACH65_RS18385 (VAWG004_36310) yggU 3971028..3971330 (+) 303 WP_088870180.1 DUF167 family protein YggU -
  AACH65_RS18390 (VAWG004_36320) - 3971374..3971793 (+) 420 WP_005357936.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41076.12 Da        Isoelectric Point: 6.5043

>NTDB_id=102979 AACH65_RS18360 WP_005340356.1 3966585..3967694(-) (pilU) [Aeromonas veronii strain VA60]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREALGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=102979 AACH65_RS18360 WP_005340356.1 3966585..3967694(-) (pilU) [Aeromonas veronii strain VA60]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGGGAAGCGCTCGACAAGAAGGGGGCGCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGCTATATCCGCACCAAGGAGGCCAACTACGCTATCTATCGCGAAGCGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCTGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAAATTCTGCAAGAGGTGGCGATGGCCAAGCGGGGGTTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACCACCCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTTCATCAACATGGTCGCAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGCTCGCTGCGTCAGGCACCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACAGGCCACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAAGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTTAATACCCCGCTCATCACCGACATTA
TCCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAAGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAACTCTCCGGTCGCGAGCGGCTCGGTACCGGTACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36

Multiple sequence alignment