Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB5B06_RS09695 Genome accession   NZ_CP163419
Coordinates   1067789..1068832 (-) Length   347 a.a.
NCBI ID   WP_416049650.1    Uniprot ID   -
Organism   Cupriavidus basilensis strain BL-MT-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1062789..1073832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB5B06_RS09665 - 1063706..1064092 (+) 387 WP_416049647.1 CidA/LrgA family protein -
  AB5B06_RS09670 - 1064089..1064811 (+) 723 WP_043349437.1 LrgB family protein -
  AB5B06_RS09675 - 1064892..1065257 (+) 366 WP_416049648.1 VOC family protein -
  AB5B06_RS09680 - 1065312..1066001 (+) 690 WP_416049649.1 helix-turn-helix transcriptional regulator -
  AB5B06_RS09685 - 1066039..1066530 (-) 492 WP_309409221.1 glutathione peroxidase -
  AB5B06_RS09690 pilU 1066615..1067754 (-) 1140 WP_043349449.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB5B06_RS09695 pilT 1067789..1068832 (-) 1044 WP_416049650.1 type IV pilus twitching motility protein PilT Machinery gene
  AB5B06_RS09700 - 1068896..1069624 (+) 729 WP_416049651.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB5B06_RS09705 proC 1069670..1070494 (+) 825 WP_416049652.1 pyrroline-5-carboxylate reductase -
  AB5B06_RS09710 ubiA 1070560..1071417 (-) 858 WP_416049653.1 4-hydroxybenzoate octaprenyltransferase -
  AB5B06_RS09715 - 1071547..1072032 (-) 486 WP_416049654.1 Dps family protein -
  AB5B06_RS09720 - 1072110..1072304 (-) 195 WP_416049655.1 hypothetical protein -
  AB5B06_RS09725 - 1072382..1073827 (-) 1446 WP_416049656.1 catalase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38404.11 Da        Isoelectric Point: 6.9771

>NTDB_id=1029596 AB5B06_RS09695 WP_416049650.1 1067789..1068832(-) (pilT) [Cupriavidus basilensis strain BL-MT-10]
MDIAQLLAFAVKNKASDLHLSADMPPMVRIHGDMRRINVAAMGHKDVHAMVYDIMSDTQRKQYEERLEIDFSFEIASLSR
FRVNAYNTQRGAAAVFRTIPSKVLTLEDLRAPAVFADLCMKPRGLVLVTGPTGSGKSTTLAAMVDHRNDNDMGHILTVED
PIEFVHTSKKSLINQRELGPHTHSFANALRSALREDPDVVLVGELRDLETIRLALTAAETGHLVFATLHTSSAAKTIDRV
VDVFPPEEKDMVRTMLSESLEAVISQTLLKTRDGNGRTAAHEIMIATPAIRHLIRENKIAQMYSMMQTSSGLGMQTLDQC
LSDLIKRGIINYNDARAIAKNPDSFTG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1029596 AB5B06_RS09695 WP_416049650.1 1067789..1068832(-) (pilT) [Cupriavidus basilensis strain BL-MT-10]
ATGGACATCGCGCAGCTATTAGCTTTCGCCGTCAAGAACAAGGCGTCCGATCTCCATCTCTCGGCAGACATGCCGCCGAT
GGTGCGTATCCACGGCGATATGCGCCGCATCAATGTGGCCGCGATGGGCCACAAAGATGTCCACGCCATGGTGTACGACA
TCATGAGCGACACCCAGCGCAAGCAATATGAGGAACGGCTGGAAATCGACTTTTCTTTCGAGATCGCCAGCCTCTCGCGA
TTCCGGGTCAATGCCTACAATACGCAGCGCGGCGCCGCCGCCGTGTTCCGGACCATTCCTTCCAAGGTACTGACGCTGGA
AGACCTGCGCGCGCCGGCGGTATTCGCTGACCTGTGCATGAAGCCGCGCGGCCTGGTGCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCCACCACGCTGGCGGCGATGGTGGACCACCGCAATGATAACGACATGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAGTTCGTGCACACGTCCAAGAAGAGCCTGATCAACCAGCGCGAGCTGGGGCCGCACACGCATTCCTTTGCCAA
CGCGCTGCGCTCCGCGCTGCGTGAGGATCCCGATGTGGTGCTGGTGGGTGAGCTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACTGCGGCCGAAACCGGCCACCTGGTGTTCGCCACACTGCACACGAGCTCGGCGGCCAAGACCATCGACCGGGTT
GTCGACGTGTTCCCGCCCGAAGAAAAAGACATGGTGCGCACCATGTTGTCGGAATCGCTGGAAGCGGTGATTTCGCAAAC
GCTGCTCAAGACGCGCGATGGCAACGGCCGTACCGCGGCGCACGAGATCATGATCGCCACGCCTGCGATCCGCCACCTGA
TCCGCGAGAACAAGATCGCGCAGATGTACTCGATGATGCAGACCAGCAGCGGCCTTGGCATGCAGACGCTGGACCAGTGC
CTGTCGGACCTGATCAAGCGCGGCATCATCAACTACAACGATGCGCGCGCCATTGCCAAGAACCCCGACTCATTCACGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

71.884

99.424

0.715
  pilT Acinetobacter baumannii D1279779

71.884

99.424

0.715
  pilT Acinetobacter baumannii strain A118

71.884

99.424

0.715
  pilT Acinetobacter baylyi ADP1

70.145

99.424

0.697
  pilT Pseudomonas stutzeri DSM 10701

69.565

99.424

0.692
  pilT Pseudomonas aeruginosa PAK

68.696

99.424

0.683
  pilT Legionella pneumophila strain Lp02

66.667

99.424

0.663
  pilT Legionella pneumophila strain ERS1305867

66.667

99.424

0.663
  pilT Neisseria gonorrhoeae MS11

66.474

99.712

0.663
  pilT Neisseria meningitidis 8013

66.474

99.712

0.663
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.282

97.118

0.634
  pilT Vibrio cholerae strain A1552

65.282

97.118

0.634
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.696

99.424

0.484
  pilU Acinetobacter baylyi ADP1

39.884

99.712

0.398
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395
  pilU Vibrio cholerae strain A1552

40

96.542

0.386

Multiple sequence alignment