Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB5B06_RS09690 Genome accession   NZ_CP163419
Coordinates   1066615..1067754 (-) Length   379 a.a.
NCBI ID   WP_043349449.1    Uniprot ID   A0A0C4YJM3
Organism   Cupriavidus basilensis strain BL-MT-10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1061615..1072754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB5B06_RS09660 glcF 1062363..1063628 (+) 1266 WP_416049646.1 glycolate oxidase subunit GlcF -
  AB5B06_RS09665 - 1063706..1064092 (+) 387 WP_416049647.1 CidA/LrgA family protein -
  AB5B06_RS09670 - 1064089..1064811 (+) 723 WP_043349437.1 LrgB family protein -
  AB5B06_RS09675 - 1064892..1065257 (+) 366 WP_416049648.1 VOC family protein -
  AB5B06_RS09680 - 1065312..1066001 (+) 690 WP_416049649.1 helix-turn-helix transcriptional regulator -
  AB5B06_RS09685 - 1066039..1066530 (-) 492 WP_309409221.1 glutathione peroxidase -
  AB5B06_RS09690 pilU 1066615..1067754 (-) 1140 WP_043349449.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB5B06_RS09695 pilT 1067789..1068832 (-) 1044 WP_416049650.1 type IV pilus twitching motility protein PilT Machinery gene
  AB5B06_RS09700 - 1068896..1069624 (+) 729 WP_416049651.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB5B06_RS09705 proC 1069670..1070494 (+) 825 WP_416049652.1 pyrroline-5-carboxylate reductase -
  AB5B06_RS09710 ubiA 1070560..1071417 (-) 858 WP_416049653.1 4-hydroxybenzoate octaprenyltransferase -
  AB5B06_RS09715 - 1071547..1072032 (-) 486 WP_416049654.1 Dps family protein -
  AB5B06_RS09720 - 1072110..1072304 (-) 195 WP_416049655.1 hypothetical protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 41980.17 Da        Isoelectric Point: 6.3709

>NTDB_id=1029595 AB5B06_RS09690 WP_043349449.1 1066615..1067754(-) (pilU) [Cupriavidus basilensis strain BL-MT-10]
MLDRESAAKYINDLLELMVGNRGSDLFITADFPPAIKVDGKITPVSQQPLNPTQALGLVRSIMNERQAGEFDGSRECNFA
ISVPNAGRFRVSAFIQQGKAGMVVRTINTRIPSVEDLDLPPALHDIVMSKRGLVIVTGATGSGKSTSLAAMLDHRNAHSY
GHIITIEDPIEYVHAHQNCIVTQREVGIDTDSWHVALKNTLRQAPDVILIGEIRDRETMEYAMQYAETGHLCLATLHANN
ANQAIDRVVNFFPEEKRQQLLIDLSLNLKAMVSQRLLPRKGQKGRVPAVEIMLGTPLVADLIFKGEIHALKEVIKKSREQ
GMVSFDQALFDLYEAGKITYEDALRNADSLNDLRLMIKLHGTMDRETDLGAGTEHLNVI

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1029595 AB5B06_RS09690 WP_043349449.1 1066615..1067754(-) (pilU) [Cupriavidus basilensis strain BL-MT-10]
ATGCTCGATCGCGAATCCGCCGCCAAGTACATCAACGACCTGCTCGAGCTGATGGTGGGCAACCGCGGCTCGGACCTGTT
CATCACCGCGGATTTTCCGCCGGCCATCAAGGTCGATGGCAAGATCACGCCAGTCTCGCAGCAGCCGCTCAACCCCACCC
AGGCGCTTGGCCTGGTGCGTTCCATCATGAACGAGCGGCAGGCGGGCGAGTTCGACGGTAGCCGCGAGTGCAACTTCGCC
ATCTCCGTGCCCAACGCCGGGCGCTTTCGCGTCTCCGCGTTCATCCAGCAGGGCAAGGCGGGCATGGTGGTGCGTACCAT
CAATACGCGCATTCCTTCGGTGGAAGACCTCGACCTGCCCCCCGCGCTGCATGACATCGTGATGTCAAAGCGCGGGCTGG
TGATCGTCACCGGCGCCACCGGCTCGGGCAAGTCGACCTCGCTCGCGGCCATGCTCGATCACCGCAACGCGCATTCGTAC
GGGCATATCATCACCATCGAGGACCCGATCGAATACGTGCACGCGCACCAGAACTGCATCGTCACGCAGCGCGAGGTCGG
CATCGATACCGACTCCTGGCACGTGGCGTTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATCGGCGAAATCC
GCGATCGGGAAACCATGGAGTACGCGATGCAGTACGCGGAAACGGGCCACCTGTGCCTGGCTACGCTGCACGCCAACAAT
GCCAACCAGGCCATCGACCGCGTGGTCAACTTCTTCCCCGAGGAAAAGCGCCAGCAGTTGCTGATCGACCTGTCGCTCAA
CCTGAAGGCGATGGTGTCGCAGCGCCTGTTGCCGCGCAAGGGGCAGAAGGGCCGGGTGCCGGCAGTGGAGATCATGCTCG
GCACGCCGCTGGTGGCCGACCTGATCTTCAAGGGCGAGATCCATGCGCTCAAGGAAGTCATCAAGAAGTCGCGCGAGCAG
GGCATGGTGTCGTTCGACCAGGCGCTGTTCGATCTCTACGAGGCCGGCAAGATCACCTACGAAGACGCGCTGCGCAATGC
CGACTCGCTCAACGACCTGCGCTTGATGATCAAGCTGCACGGCACCATGGACCGCGAGACCGACCTGGGCGCCGGCACCG
AACACCTGAATGTGATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C4YJM3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.173

93.14

0.588
  pilU Acinetobacter baylyi ADP1

57.382

94.723

0.544
  pilU Vibrio cholerae strain A1552

53.719

95.778

0.515
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

88.918

0.398
  pilT Acinetobacter baylyi ADP1

43.363

89.446

0.388
  pilT Legionella pneumophila strain Lp02

44.144

87.863

0.388
  pilT Legionella pneumophila strain ERS1305867

44.144

87.863

0.388
  pilT Acinetobacter nosocomialis M2

43.844

87.863

0.385
  pilT Acinetobacter baumannii D1279779

43.844

87.863

0.385
  pilT Acinetobacter baumannii strain A118

43.844

87.863

0.385
  pilT Pseudomonas aeruginosa PAK

43.027

88.918

0.383
  pilT Pseudomonas stutzeri DSM 10701

43.027

88.918

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.218

83.641

0.361
  pilT Vibrio cholerae strain A1552

43.218

83.641

0.361

Multiple sequence alignment