Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AACH75_RS17660 Genome accession   NZ_AP027933
Coordinates   3806656..3807690 (-) Length   344 a.a.
NCBI ID   WP_005340354.1    Uniprot ID   -
Organism   Aeromonas veronii strain VA7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3801656..3812690
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH75_RS17630 (VAWG002_35030) glnK 3802238..3802576 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  AACH75_RS17635 (VAWG002_35040) arfB 3802778..3803194 (+) 417 WP_216959480.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  AACH75_RS17640 (VAWG002_35050) - 3803225..3803716 (+) 492 WP_216949693.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  AACH75_RS17645 (VAWG002_35060) - 3804077..3804502 (-) 426 WP_040098961.1 hypothetical protein -
  AACH75_RS17650 (VAWG002_35070) yaaA 3804697..3805470 (-) 774 WP_040067414.1 peroxide stress protein YaaA -
  AACH75_RS17655 (VAWG002_35080) pilU 3805518..3806627 (-) 1110 WP_005357951.1 type IVa pilus ATPase TapU Machinery gene
  AACH75_RS17660 (VAWG002_35090) pilT 3806656..3807690 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  AACH75_RS17665 (VAWG002_35100) - 3807730..3808431 (+) 702 WP_204480235.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACH75_RS17670 (VAWG002_35110) proC 3808574..3809398 (+) 825 WP_216949695.1 pyrroline-5-carboxylate reductase -
  AACH75_RS17675 (VAWG002_35120) - 3809410..3809961 (+) 552 WP_042011910.1 YggT family protein -
  AACH75_RS17680 (VAWG002_35130) yggU 3809961..3810263 (+) 303 WP_005340341.1 DUF167 family protein YggU -
  AACH75_RS17685 (VAWG002_35140) - 3810307..3810726 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -
  AACH75_RS17690 (VAWG002_35150) - 3810828..3812024 (-) 1197 WP_005348874.1 NupC/NupG family nucleoside CNT transporter -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38273.03 Da        Isoelectric Point: 6.7440

>NTDB_id=102923 AACH75_RS17660 WP_005340354.1 3806656..3807690(-) (pilT) [Aeromonas veronii strain VA7]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGEVRKINLPALEHREVHALIYDIMNDHQRKELEENFEVDFSFEVPNLAR
FRVNAFQQARGSGAVFRTIPSTVLSLEDLDAPEIFRKIAEFPRGLVLVTGPTGSGKSTTLAAMVNYINENFHHHILTIED
PIEFVHENKRCLVNQREVHRDTKSFSNALRSALREDPDIILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKRIGGGRVAAHEIMMGIPAVRNLIREDKIAQLYSVIQTGMTHGMQTMDQSL
KQLVSRGVVASLDAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=102923 AACH75_RS17660 WP_005340354.1 3806656..3807690(-) (pilT) [Aeromonas veronii strain VA7]
ATGGATATCACAGAGTTATTGGCTTTCAGTGTAAAGCATAAAGCCTCGGATCTACACCTCTCGGCCGGGGTTCCCCCGAT
GATCAGGGTTGATGGTGAGGTTCGCAAGATCAATTTGCCCGCCCTGGAACACCGGGAAGTGCATGCCCTCATTTACGACA
TCATGAACGACCATCAGCGCAAGGAGCTGGAGGAGAACTTCGAGGTCGACTTCTCGTTCGAGGTGCCCAATCTGGCCCGT
TTCCGGGTCAACGCCTTCCAGCAGGCTCGCGGCTCGGGCGCGGTATTTCGTACCATCCCCAGCACTGTGCTGAGCCTCGA
GGATCTCGATGCGCCGGAGATCTTCCGCAAGATCGCCGAGTTCCCGCGGGGTCTGGTGTTGGTGACCGGCCCGACAGGTT
CCGGTAAATCGACCACCCTGGCTGCCATGGTCAATTACATCAACGAGAACTTCCATCACCACATCCTCACCATCGAAGAC
CCCATCGAATTCGTCCACGAGAACAAGCGCTGTCTGGTGAACCAGCGGGAAGTGCACCGGGATACCAAGAGCTTCAGCAA
CGCTCTGCGCTCGGCACTGCGGGAAGACCCGGACATCATTCTGGTGGGCGAGATGCGCGACCTTGAGACCATTCGTCTGG
CCATGACCGCCGCCGAAACTGGCCACCTGGTGTTTGGCACCCTGCACACCTCGTCGGCGGCCAAGACCATCGACCGTATC
ATCGACGTCTTCCCCGGCGCAGAGAAAGACATGGTGCGCTCCATGCTCTCCGAATCCCTGCGGGCGGTTATCTCCCAGAC
CCTGCTCAAGCGCATTGGCGGTGGCCGGGTGGCGGCTCACGAGATCATGATGGGCATTCCAGCGGTGCGTAACCTGATCC
GCGAGGACAAGATTGCCCAGCTCTACTCGGTGATCCAGACCGGGATGACCCACGGCATGCAGACCATGGATCAGAGTCTC
AAGCAGTTGGTGAGTCGTGGTGTGGTGGCATCCCTCGATGCCAAGGCCAAGGCTGTTGATCCCAACAGCATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

77.326

100

0.773
  pilT Acinetobacter baylyi ADP1

77.551

99.709

0.773
  pilT Acinetobacter baumannii D1279779

77.035

100

0.77
  pilT Acinetobacter baumannii strain A118

77.035

100

0.77
  pilT Pseudomonas stutzeri DSM 10701

76.163

100

0.762
  pilT Pseudomonas aeruginosa PAK

75.291

100

0.753
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

75.964

97.965

0.744
  pilT Vibrio cholerae strain A1552

75.964

97.965

0.744
  pilT Legionella pneumophila strain Lp02

72.941

98.837

0.721
  pilT Legionella pneumophila strain ERS1305867

72.941

98.837

0.721
  pilT Neisseria meningitidis 8013

69.855

100

0.701
  pilT Neisseria gonorrhoeae MS11

69.565

100

0.698
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.754

99.419

0.515
  pilU Vibrio cholerae strain A1552

42.899

100

0.43
  pilU Pseudomonas stutzeri DSM 10701

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.462

100

0.407

Multiple sequence alignment