Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AACH75_RS17655 Genome accession   NZ_AP027933
Coordinates   3805518..3806627 (-) Length   369 a.a.
NCBI ID   WP_005357951.1    Uniprot ID   -
Organism   Aeromonas veronii strain VA7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3800518..3811627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AACH75_RS17625 (VAWG002_35020) - 3800972..3801979 (-) 1008 WP_005340374.1 Fe(3+) ABC transporter substrate-binding protein -
  AACH75_RS17630 (VAWG002_35030) glnK 3802238..3802576 (-) 339 WP_005341406.1 P-II family nitrogen regulator -
  AACH75_RS17635 (VAWG002_35040) arfB 3802778..3803194 (+) 417 WP_216959480.1 alternative ribosome rescue aminoacyl-tRNA hydrolase ArfB -
  AACH75_RS17640 (VAWG002_35050) - 3803225..3803716 (+) 492 WP_216949693.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  AACH75_RS17645 (VAWG002_35060) - 3804077..3804502 (-) 426 WP_040098961.1 hypothetical protein -
  AACH75_RS17650 (VAWG002_35070) yaaA 3804697..3805470 (-) 774 WP_040067414.1 peroxide stress protein YaaA -
  AACH75_RS17655 (VAWG002_35080) pilU 3805518..3806627 (-) 1110 WP_005357951.1 type IVa pilus ATPase TapU Machinery gene
  AACH75_RS17660 (VAWG002_35090) pilT 3806656..3807690 (-) 1035 WP_005340354.1 type IVa pilus ATPase TapT Machinery gene
  AACH75_RS17665 (VAWG002_35100) - 3807730..3808431 (+) 702 WP_204480235.1 YggS family pyridoxal phosphate-dependent enzyme -
  AACH75_RS17670 (VAWG002_35110) proC 3808574..3809398 (+) 825 WP_216949695.1 pyrroline-5-carboxylate reductase -
  AACH75_RS17675 (VAWG002_35120) - 3809410..3809961 (+) 552 WP_042011910.1 YggT family protein -
  AACH75_RS17680 (VAWG002_35130) yggU 3809961..3810263 (+) 303 WP_005340341.1 DUF167 family protein YggU -
  AACH75_RS17685 (VAWG002_35140) - 3810307..3810726 (+) 420 WP_005340339.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41104.17 Da        Isoelectric Point: 6.5043

>NTDB_id=102922 AACH75_RS17655 WP_005357951.1 3805518..3806627(-) (pilU) [Aeromonas veronii strain VA7]
MNLDDLLSELVERKGSDLFVTVGSPPTLKVNGHLVSLGGEALDKKGALTLVRDTLSSDHFERYIRTKEANYAIYREVLGR
FRVSAFWQQELPGMVVRRIETRIPTFEDLQLPKILQEVAMAKRGLVLFVGATGAGKSTTQAAMIGYRNQHADGHILTVED
PVEFVHQHGRSLVTQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQDKHRQFLFDLSFNLKAIVAQQLVPSMDGKRRCAAFEILLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFSLFCAGQIGYSEALAHADSANDLRLLIKLSGRERLGTGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=102922 AACH75_RS17655 WP_005357951.1 3805518..3806627(-) (pilU) [Aeromonas veronii strain VA7]
ATGAATCTGGATGATCTGCTGAGCGAGCTGGTCGAGCGAAAGGGATCGGATCTGTTTGTGACGGTGGGCTCGCCGCCCAC
CCTCAAGGTGAATGGCCATCTGGTGTCGCTGGGGGGCGAAGCGCTCGACAAGAAGGGAGCTCTGACGCTGGTCAGGGATA
CCCTCAGCAGCGATCACTTCGAGCGTTATATCCGTACCAAGGAGGCCAACTACGCGATCTATCGCGAGGTGCTTGGCCGT
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCCGGCATGGTGGTGCGGCGTATCGAGACCCGCATTCCCACCTTTGA
AGATCTGCAGCTACCCAAGATCCTGCAAGAGGTGGCGATGGCCAAGCGGGGGCTGGTGCTGTTTGTCGGCGCCACCGGGG
CGGGCAAATCGACTACTCAGGCGGCGATGATCGGCTATCGCAACCAGCATGCCGATGGTCACATTCTGACGGTGGAAGAT
CCGGTGGAGTTCGTCCATCAGCATGGTCGTAGTCTGGTGACCCAGCGAGAGGTGGGGATCGACACCGAGTCGTTCGATGT
GGCGCTGAAAAGTTCGCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGAAGTCAGGAGACCATGGAGTTTG
CCCTGCAGTTCGCCGAGACAGGACACCTTTGTCTCGCCACTTTGCATGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAGGACAAACACCGCCAGTTCCTGTTCGATCTCTCTTTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGCCGAGTATGGATGGCAAGCGGCGCTGCGCTGCGTTCGAGATCCTGCTCAATACTCCGCTCATCACTGACATTA
TTCGCAAGGGAGAGATGCATCGCCTCAAGGAGGTGATGACCAAATCCACCGAGCTGGGTATGCAGACCTTCGATCAAGCG
CTCTTCAGCCTGTTCTGCGCCGGCCAGATTGGCTACAGTGAGGCACTCGCCCACGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTCTCCGGTCGCGAGCGGCTCGGTACCGGCACGCTGGACAATGTGACGCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.326

99.729

0.602
  pilU Pseudomonas stutzeri DSM 10701

60.399

95.122

0.575
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.816

92.954

0.379
  pilT Legionella pneumophila strain ERS1305867

40.816

92.954

0.379
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.823

91.87

0.366
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363
  pilT Vibrio cholerae strain A1552

39.82

90.515

0.36
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.82

90.515

0.36

Multiple sequence alignment