Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QUE46_RS02820 Genome accession   NZ_AP027922
Coordinates   645396..646436 (+) Length   346 a.a.
NCBI ID   WP_004586840.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. MM1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 640396..651436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE46_RS02790 (PspMM1_05500) hemW 641044..642180 (-) 1137 WP_286246127.1 radical SAM family heme chaperone HemW -
  QUE46_RS02795 (PspMM1_05510) - 642177..642776 (-) 600 WP_286246128.1 XTP/dITP diphosphatase -
  QUE46_RS02800 (PspMM1_05520) - 642781..643224 (-) 444 WP_286246129.1 DUF4426 domain-containing protein -
  QUE46_RS02805 (PspMM1_05530) - 643281..643814 (-) 534 WP_004586837.1 YggT family protein -
  QUE46_RS02810 (PspMM1_05540) proC 643841..644662 (-) 822 WP_004586838.1 pyrroline-5-carboxylate reductase -
  QUE46_RS02815 (PspMM1_05550) - 644680..645393 (-) 714 WP_286246130.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUE46_RS02820 (PspMM1_05560) pilT 645396..646436 (+) 1041 WP_004586840.1 type IV pilus twitching motility protein PilT Machinery gene
  QUE46_RS02825 (PspMM1_05570) pilU 646446..647579 (+) 1134 WP_286246131.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUE46_RS02830 (PspMM1_05580) ruvX 647666..648115 (-) 450 WP_286246132.1 Holliday junction resolvase RuvX -
  QUE46_RS02835 (PspMM1_05590) - 648125..648682 (-) 558 WP_286246133.1 YqgE/AlgH family protein -
  QUE46_RS02840 (PspMM1_05600) gshB 648807..649757 (-) 951 WP_286246134.1 glutathione synthase -
  QUE46_RS02845 (PspMM1_05610) rsmE 649812..650546 (-) 735 WP_286246135.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QUE46_RS02850 (PspMM1_05620) - 650619..651095 (-) 477 WP_286246136.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38141.37 Da        Isoelectric Point: 6.1844

>NTDB_id=102831 QUE46_RS02820 WP_004586840.1 645396..646436(+) (pilT) [Pseudoalteromonas sp. MM1]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPALADKDVNSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSDVLTLEDLGAPDIFKTISDNPRGLVLVTGPTGSGKSTTLAAMVDYINQTKHHHILTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMVRSMLSESLRAVISQTLLKKIGGGRVAAHEIMIGVPAIRNLIREDKIAQMYSSIQTGASHGMQTMDQCL
NNLVNHGIVTTAAAKEKAQDKTQFGG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=102831 QUE46_RS02820 WP_004586840.1 645396..646436(+) (pilT) [Pseudoalteromonas sp. MM1]
ATGGATATTACCGAATTATTAGCGTTTAGTGTGCAACACAAAGCATCTGATTTACACTTATCGTCAGGTGTTTCACCTAT
GATACGCGTAGATGGTGATGTTCGCCGCATTAATATACCTGCACTTGCAGACAAAGACGTAAACAGCCTTGTTTACGACA
TTATGAACGATAATCAGCGCAAGGACTACGAACAAAACCTAGAAGTGGATTTTTCGTTTGAAGTGCCTAACCTTGCGCGT
TTTCGTGTTAACGCATTTAACTCAAACCGCGGCCCTGCAGCGGTGTTTCGTACCATCCCAAGCGATGTACTGACGCTTGA
GGATTTAGGTGCGCCAGATATTTTTAAAACAATATCCGATAACCCGCGTGGTTTAGTATTGGTTACCGGCCCAACGGGTT
CAGGTAAATCAACAACACTTGCGGCTATGGTCGATTATATAAATCAAACAAAACATCACCATATCCTCACCATTGAAGAT
CCTATCGAATTTGTTCACGATAATAAACTCAGCCTTATTAACCAACGTGAAGTGCACCGAGATACGCACAGCTTTTCTAA
TGCATTGCGCAGCGCGCTGCGTGAAGACCCCGATGTAATACTTGTCGGTGAGCTACGTGACTTAGAAACCATTCGCCTTG
CGATGACCGCCGCTGAAACCGGTCACTTAGTGTTTGGTACGCTACACACCACCTCGGCACCAAAAACCATTGACCGTATT
ATTGATGTATTCCCAGGTGAAGAAAAAGATATGGTGCGCTCAATGCTTTCTGAGTCGTTACGTGCCGTTATTTCGCAAAC
ACTGCTTAAAAAAATTGGCGGTGGACGAGTGGCCGCGCACGAAATTATGATTGGTGTGCCGGCGATTCGTAACTTAATAC
GTGAAGATAAAATTGCACAAATGTATTCGTCTATTCAAACGGGTGCATCACACGGTATGCAAACCATGGATCAATGTTTA
AACAATTTAGTTAATCACGGCATTGTGACAACCGCAGCCGCAAAAGAAAAAGCCCAAGATAAAACCCAGTTTGGTGGCTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

77.326

99.422

0.769
  pilT Acinetobacter nosocomialis M2

77.035

99.422

0.766
  pilT Acinetobacter baumannii D1279779

77.035

99.422

0.766
  pilT Acinetobacter baumannii strain A118

77.035

99.422

0.766
  pilT Pseudomonas aeruginosa PAK

76.453

99.422

0.76
  pilT Acinetobacter baylyi ADP1

76.453

99.422

0.76
  pilT Legionella pneumophila strain Lp02

72.384

99.422

0.72
  pilT Legionella pneumophila strain ERS1305867

72.384

99.422

0.72
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.386

97.977

0.699
  pilT Vibrio cholerae strain A1552

71.386

97.977

0.699
  pilT Neisseria gonorrhoeae MS11

70.674

98.555

0.697
  pilT Neisseria meningitidis 8013

70.674

98.555

0.697
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

99.711

0.52
  pilU Vibrio cholerae strain A1552

42.462

93.931

0.399
  pilU Acinetobacter baylyi ADP1

38.824

98.266

0.382
  pilU Pseudomonas stutzeri DSM 10701

39.879

95.665

0.382

Multiple sequence alignment