Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QUE46_RS02825 Genome accession   NZ_AP027922
Coordinates   646446..647579 (+) Length   377 a.a.
NCBI ID   WP_286246131.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. MM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 641446..652579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE46_RS02795 (PspMM1_05510) - 642177..642776 (-) 600 WP_286246128.1 XTP/dITP diphosphatase -
  QUE46_RS02800 (PspMM1_05520) - 642781..643224 (-) 444 WP_286246129.1 DUF4426 domain-containing protein -
  QUE46_RS02805 (PspMM1_05530) - 643281..643814 (-) 534 WP_004586837.1 YggT family protein -
  QUE46_RS02810 (PspMM1_05540) proC 643841..644662 (-) 822 WP_004586838.1 pyrroline-5-carboxylate reductase -
  QUE46_RS02815 (PspMM1_05550) - 644680..645393 (-) 714 WP_286246130.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUE46_RS02820 (PspMM1_05560) pilT 645396..646436 (+) 1041 WP_004586840.1 type IV pilus twitching motility protein PilT Machinery gene
  QUE46_RS02825 (PspMM1_05570) pilU 646446..647579 (+) 1134 WP_286246131.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUE46_RS02830 (PspMM1_05580) ruvX 647666..648115 (-) 450 WP_286246132.1 Holliday junction resolvase RuvX -
  QUE46_RS02835 (PspMM1_05590) - 648125..648682 (-) 558 WP_286246133.1 YqgE/AlgH family protein -
  QUE46_RS02840 (PspMM1_05600) gshB 648807..649757 (-) 951 WP_286246134.1 glutathione synthase -
  QUE46_RS02845 (PspMM1_05610) rsmE 649812..650546 (-) 735 WP_286246135.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QUE46_RS02850 (PspMM1_05620) - 650619..651095 (-) 477 WP_286246136.1 hypothetical protein -
  QUE46_RS02855 (PspMM1_05630) - 651177..652070 (-) 894 WP_286246137.1 hypothetical protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41778.68 Da        Isoelectric Point: 6.1358

>NTDB_id=102832 QUE46_RS02825 WP_286246131.1 646446..647579(+) (pilU) [Pseudoalteromonas sp. MM1]
MNLSLNHFLQIMIEKKGSDLFVSSQLPVSAKINGELIALSDDKLTDEQSLALVESAMSDKQKNEFHTTKECNFAIATDEG
RFRISAFWQRDRAGMVIRRIVTQIPDVSDLGLPSTLTDVIMAKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHRKSIITQREVGLDTDSFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIVAQQLIPTSDGEGRVAAIEILLNSPMVAELIKNGDIGGIKEAMSKSKEMGMQTFDQ
ALFELYKQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKNGN

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=102832 QUE46_RS02825 WP_286246131.1 646446..647579(+) (pilU) [Pseudoalteromonas sp. MM1]
ATGAATTTATCTTTAAATCACTTTTTGCAAATAATGATTGAGAAAAAAGGCTCGGATTTATTTGTTTCTAGCCAATTGCC
AGTCAGCGCAAAAATTAATGGTGAGCTTATCGCTCTTAGCGACGATAAGCTAACTGATGAGCAATCGCTTGCGCTTGTCG
AGTCAGCGATGAGCGATAAACAAAAAAACGAATTTCATACCACTAAAGAGTGTAACTTTGCGATAGCCACAGACGAAGGG
CGTTTTCGTATATCGGCTTTTTGGCAGCGTGATAGAGCGGGTATGGTTATTCGCCGTATTGTAACGCAAATACCTGATGT
AAGTGATTTAGGCCTGCCCTCAACACTTACCGATGTAATTATGGCTAAGCGCGGGTTAGTGCTGTTTGTGGGGGGAACTG
GCACAGGTAAATCAACCTCATTGGCTGCTTTAATTGGCTACAGAAACCGCAACCAACGCGGCCATATACTCACTATTGAA
GACCCAATTGAGTTTGTACACGAGCACCGTAAAAGTATTATTACTCAGCGCGAAGTAGGGCTTGATACCGACAGCTTTGA
ATCGGCACTAAAAAGCTCTTTACGCCAAGCTCCCGATGTTATTTTAATTGGTGAAATACGCTCACAAGAGACCATGGAAT
ACGCACTGAGCTTTGCCGAGACGGGCCACTTATGTGTGGCTACACTGCATGCTAATAATGCCAACCAAGCCATTGACCGT
ATTATGCACTTAGTCCCTAAAGAAAAGCACGACAAGCTCAAGTACGACTTAGCGCTAAATTTACGCGCCATTGTTGCTCA
GCAGCTTATTCCAACGTCTGATGGGGAAGGGCGAGTTGCCGCGATTGAAATATTATTAAACTCACCCATGGTGGCAGAGC
TAATTAAAAATGGGGATATTGGTGGCATTAAAGAGGCTATGTCTAAGTCTAAAGAAATGGGTATGCAAACCTTTGACCAA
GCCTTATTTGAGCTATATAAACAGCAACGTATTAATTACGCCGATGCGCTGCATCATGCTGATTCGCCAAACGATTTACG
CTTAATGATTAAGCTGCGCAATAACGAGCAACAAGGCGCAGGCTTTTTACAAGGTGTAACCATTGATGGACTCGATGATA
AAAACGGTAATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.614

100

0.568
  pilU Acinetobacter baylyi ADP1

55.968

100

0.56
  pilU Vibrio cholerae strain A1552

55.858

97.347

0.544
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.105

95.756

0.403

Multiple sequence alignment