Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB3N45_RS18515 Genome accession   NZ_CP162159
Coordinates   3966476..3967456 (-) Length   326 a.a.
NCBI ID   WP_029883139.1    Uniprot ID   A0AAE2JRL6
Organism   Enterobacter cloacae strain STK39-C     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3961476..3972456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3N45_RS18485 (AB3N45_18490) - 3962352..3962849 (+) 498 WP_028027997.1 SprT family zinc-dependent metalloprotease -
  AB3N45_RS18490 (AB3N45_18495) endA 3962944..3963651 (+) 708 WP_013098661.1 deoxyribonuclease I -
  AB3N45_RS18495 (AB3N45_18500) rsmE 3963704..3964435 (+) 732 WP_320724952.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB3N45_RS18500 (AB3N45_18505) gshB 3964455..3965402 (+) 948 WP_023620782.1 glutathione synthase -
  AB3N45_RS18505 (AB3N45_18510) - 3965489..3966049 (+) 561 WP_013098664.1 YqgE/AlgH family protein -
  AB3N45_RS18510 (AB3N45_18515) ruvX 3966049..3966465 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  AB3N45_RS18515 (AB3N45_18520) pilT 3966476..3967456 (-) 981 WP_029883139.1 type IV pilus twitching motility protein PilT Machinery gene
  AB3N45_RS18520 (AB3N45_18525) - 3967474..3968175 (+) 702 WP_320724953.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB3N45_RS18525 (AB3N45_18530) - 3968197..3968763 (+) 567 WP_013098667.1 YggT family protein -
  AB3N45_RS18530 (AB3N45_18535) yggU 3968760..3969047 (+) 288 WP_020687057.1 DUF167 family protein YggU -
  AB3N45_RS18535 (AB3N45_18540) - 3969060..3969653 (+) 594 WP_320724954.1 XTP/dITP diphosphatase -
  AB3N45_RS18540 (AB3N45_18545) hemW 3969646..3970794 (+) 1149 WP_095427932.1 radical SAM family heme chaperone HemW -
  AB3N45_RS18545 (AB3N45_18550) - 3970830..3970949 (+) 120 Protein_3626 DUF559 domain-containing protein -
  AB3N45_RS18550 (AB3N45_18555) - 3971017..3971733 (-) 717 WP_320724955.1 DUF2884 domain-containing protein -
  AB3N45_RS18555 (AB3N45_18560) - 3971790..3972116 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35652.66 Da        Isoelectric Point: 6.3038

>NTDB_id=1024415 AB3N45_RS18515 WP_029883139.1 3966476..3967456(-) (pilT) [Enterobacter cloacae strain STK39-C]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRVGRLEPAPFPSPDVDALLKTWLNDEQQGAWWASGQVDFAVTLTGNQRLRA
SAFKQMKGNSITLRLLPRACPQLSALGVPRAIPELLSHDSGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FVYQSERCLIQQREIGLHSPSFAEALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1024415 AB3N45_RS18515 WP_029883139.1 3966476..3967456(-) (pilT) [Enterobacter cloacae strain STK39-C]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGAGTAGGCCGCCTTGAACCGGCTCCCTTTCCCTCTCCCGATGTGGATGCGTTACTGAAAACGTGGCTCAACG
ATGAGCAGCAGGGGGCATGGTGGGCAAGCGGGCAGGTGGATTTTGCCGTGACCCTTACGGGAAACCAGCGCCTGCGCGCA
AGCGCCTTTAAACAGATGAAGGGCAACTCCATCACGCTACGCCTGTTGCCCCGGGCCTGTCCGCAGCTCTCCGCTTTGGG
TGTTCCCCGGGCTATCCCGGAACTCTTATCCCACGACAGTGGGCTGATACTGGTGACGGGGGCAACCGGCAGCGGCAAAT
CTACCACCCTTGCGGCAATGGTGGATTTTCTTAATCACCAGACGGATGGGCATATTCTGACGCTCGAAGATCCGGTGGAG
TTTGTCTATCAGAGCGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCTGCACAGTCCCTCATTTGCCGAGGCGCTTCG
CGCTGCGTTGCGTGAAGATCCGGATGTCATCCTGCTGGGAGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CCGCTGAAACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGTGCCTCTCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGACCCGGTGCGTAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAGACTGCTTCC
CGACCTGCAGGGTGGTCGCGTCGCGCTGTATGAGCTGCTGGTGAATACGCCAGCGGCAGCGAACCTTATCCGCGAAGGTA
AAACGTGGCAGTTGCCCGGGATTATTCAAACCGGTCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCAGAGCGA
CGGGCGCAGGGGCGGTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilB Haemophilus influenzae 86-028NP

33.708

100

0.368
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment