Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB3N42_RS19210 Genome accession   NZ_CP162154
Coordinates   4056619..4057599 (-) Length   326 a.a.
NCBI ID   WP_368267555.1    Uniprot ID   -
Organism   Enterobacter cloacae strain STK71-C     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4051619..4062599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3N42_RS19180 (AB3N42_19180) - 4052495..4052992 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  AB3N42_RS19185 (AB3N42_19185) endA 4053087..4053794 (+) 708 WP_013098661.1 deoxyribonuclease I -
  AB3N42_RS19190 (AB3N42_19190) rsmE 4053847..4054578 (+) 732 WP_013098662.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB3N42_RS19195 (AB3N42_19195) gshB 4054598..4055545 (+) 948 WP_023620782.1 glutathione synthase -
  AB3N42_RS19200 (AB3N42_19200) - 4055632..4056192 (+) 561 WP_013098664.1 YqgE/AlgH family protein -
  AB3N42_RS19205 (AB3N42_19205) ruvX 4056192..4056608 (+) 417 WP_077580088.1 Holliday junction resolvase RuvX -
  AB3N42_RS19210 (AB3N42_19210) pilT 4056619..4057599 (-) 981 WP_368267555.1 type IV pilus twitching motility protein PilT Machinery gene
  AB3N42_RS19215 (AB3N42_19215) - 4057617..4058318 (+) 702 WP_023620785.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB3N42_RS19220 (AB3N42_19220) - 4058340..4058906 (+) 567 WP_013098667.1 YggT family protein -
  AB3N42_RS19225 (AB3N42_19225) yggU 4058903..4059190 (+) 288 WP_013098668.1 DUF167 family protein YggU -
  AB3N42_RS19230 (AB3N42_19230) - 4059203..4059796 (+) 594 WP_013098669.1 XTP/dITP diphosphatase -
  AB3N42_RS19235 (AB3N42_19235) hemW 4059789..4060937 (+) 1149 WP_368267556.1 radical SAM family heme chaperone HemW -
  AB3N42_RS19240 (AB3N42_19240) - 4060994..4061332 (+) 339 WP_368267557.1 endonuclease domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35637.68 Da        Isoelectric Point: 6.3038

>NTDB_id=1024326 AB3N42_RS19210 WP_368267555.1 4056619..4057599(-) (pilT) [Enterobacter cloacae strain STK71-C]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRVGRLEPAPFPSPDVDALLKTWLNDEQQGAWWASGQVDFAVTLTGNQRLRA
SAFKQMKGNSITLRLLPRACPQLSALGVPRAIPELLSHDSGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FVYQSERCLIQLREIGLHSPSFAEALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1024326 AB3N42_RS19210 WP_368267555.1 4056619..4057599(-) (pilT) [Enterobacter cloacae strain STK71-C]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGAGTAGGCCGCCTTGAACCGGCTCCCTTTCCCTCTCCCGATGTGGATGCGTTACTGAAAACGTGGCTCAACG
ATGAGCAGCAGGGGGCATGGTGGGCAAGCGGGCAGGTGGATTTTGCCGTGACACTTACGGGAAACCAGCGCCTGCGCGCA
AGCGCCTTTAAACAGATGAAGGGCAACTCAATCACGCTACGCCTGTTGCCCCGGGCCTGTCCGCAGCTCTCCGCCCTGGG
GGTTCCCCGGGCTATCCCGGAACTCTTATCCCACGACAGCGGGCTGATACTGGTGACGGGGGCAACCGGCAGCGGCAAAT
CTACCACCCTTGCGGCAATGGTGGATTTTCTTAATCACCAGACGGATGGGCATATTCTGACGCTGGAAGATCCGGTGGAG
TTTGTCTATCAGAGTGAACGTTGCCTGATTCAGCTGCGGGAGATAGGCCTGCACAGTCCCTCATTTGCCGAGGCGCTGCG
CGCTGCGTTGCGTGAAGATCCGGATGTCATCCTGCTGGGAGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CCGCTGAAACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGTGCCTCTCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGACCCGGTGCGTAATCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAGACTGCTTCC
TGACCTGCAGGGTGGGCGCGTCGCGCTGTATGAACTGCTGGTGAATACGCCAGCGGCAGCGAATCTTATCCGCGAAGGTA
AAACGTGGCAGTTGCCCGGGATTATTCAAACCGGTCAGCAGGCGGGGATGCAGAACTTTGACCAGAGCCTGGCAGAGCGA
CGGGCGCAGGGGCGGTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(63-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.847

100

0.5
  pilT Vibrio cholerae strain A1552

49.847

100

0.5
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Acinetobacter nosocomialis M2

48.125

98.16

0.472
  pilT Neisseria meningitidis 8013

46.951

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.646

100

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386
  pilB Haemophilus influenzae 86-028NP

33.708

100

0.368

Multiple sequence alignment