Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB2S62_RS12935 Genome accession   NZ_CP162100
Coordinates   2882568..2883605 (-) Length   345 a.a.
NCBI ID   WP_367987381.1    Uniprot ID   -
Organism   Vibrio sp. NTOU-M3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2877568..2888605
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB2S62_RS12905 (AB2S62_12660) endA 2877822..2878523 (+) 702 WP_367987375.1 deoxyribonuclease I -
  AB2S62_RS12910 (AB2S62_12665) rsmE 2878648..2879379 (+) 732 WP_367987376.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB2S62_RS12915 (AB2S62_12670) gshB 2879393..2880340 (+) 948 WP_367987377.1 glutathione synthase -
  AB2S62_RS12920 (AB2S62_12675) - 2880382..2880945 (+) 564 WP_367987378.1 YqgE/AlgH family protein -
  AB2S62_RS12925 (AB2S62_12680) ruvX 2880962..2881387 (+) 426 WP_367987379.1 Holliday junction resolvase RuvX -
  AB2S62_RS12930 (AB2S62_12685) pilU 2881449..2882555 (-) 1107 WP_367987380.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB2S62_RS12935 (AB2S62_12690) pilT 2882568..2883605 (-) 1038 WP_367987381.1 type IV pilus twitching motility protein PilT Machinery gene
  AB2S62_RS12940 (AB2S62_12695) - 2883631..2884347 (+) 717 WP_367987382.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB2S62_RS12945 (AB2S62_12700) proC 2884370..2885188 (+) 819 WP_367987383.1 pyrroline-5-carboxylate reductase -
  AB2S62_RS12950 (AB2S62_12705) - 2885233..2885790 (+) 558 WP_367987384.1 YggT family protein -
  AB2S62_RS12955 (AB2S62_12710) yggU 2885790..2886080 (+) 291 WP_367987385.1 DUF167 family protein YggU -
  AB2S62_RS12960 (AB2S62_12715) - 2886123..2886554 (+) 432 WP_367987386.1 DUF4426 domain-containing protein -
  AB2S62_RS12965 (AB2S62_12720) - 2886584..2887180 (+) 597 WP_367987387.1 XTP/dITP diphosphatase -
  AB2S62_RS12970 (AB2S62_12725) hemW 2887180..2888355 (+) 1176 WP_367987388.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38231.80 Da        Isoelectric Point: 6.2342

>NTDB_id=1024055 AB2S62_RS12935 WP_367987381.1 2882568..2883605(-) (pilT) [Vibrio sp. NTOU-M3]
MDIAELLDFSVKHKASDLHLSAGVSPMVRIDGEVRKLGVPAFSHADVHKLVFEIMNDAQRSEFEEKLEVDFSFELPNVGR
FRVNAFNQSRGCAAVFRTIPTDIPTLEQLETPPVFEKIANMEKGLVLVTGPTGSGKSTTLAAMVDYINRNHNKHILTIED
PIEFVHTNNKCLINQREVHRDTYSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRLGGGRVACHEIMMATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLLAQGTVDPEEISKKIETDSGLF

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1024055 AB2S62_RS12935 WP_367987381.1 2882568..2883605(-) (pilT) [Vibrio sp. NTOU-M3]
ATGGATATCGCGGAATTACTGGATTTTAGTGTAAAGCATAAAGCTTCAGATCTACACCTTTCTGCTGGCGTATCTCCAAT
GGTACGTATTGATGGTGAAGTAAGAAAGCTTGGTGTGCCCGCTTTTAGCCATGCTGATGTACATAAATTGGTTTTTGAGA
TCATGAATGACGCTCAGCGTAGTGAATTTGAAGAGAAACTGGAAGTCGATTTTTCGTTTGAGTTACCCAATGTTGGCCGT
TTTCGTGTCAATGCGTTCAACCAATCACGTGGTTGTGCGGCGGTGTTCCGTACCATTCCAACCGATATTCCCACGCTAGA
GCAGCTTGAAACCCCGCCTGTATTTGAAAAGATTGCCAATATGGAAAAAGGGTTGGTGTTGGTCACCGGACCGACTGGCT
CTGGTAAGTCGACCACGCTTGCCGCCATGGTGGATTACATTAACCGTAATCACAACAAGCACATTCTGACCATCGAAGAC
CCGATTGAATTTGTGCATACCAATAACAAATGCTTGATTAACCAAAGGGAAGTACATCGAGATACTTACAGCTTTAAAAA
TGCACTGCGTAGCGCGTTGCGTGAAGATCCAGACGTGATTTTAGTCGGTGAGTTACGCGACCAAGAAACCATCAGCTTGG
CGTTAACGGCAGCAGAAACGGGGCACTTAGTTTTTGGCACCTTGCACACCAGTTCGGCAGCGAAAACCATTGACCGTATT
ATCGATGTGTTCCCTGGCAGTGATAAAGACATGGTACGTTCAATGCTGTCAGAGTCATTGCGTGCGGTTATTGCTCAGAA
ACTATTAAAACGCTTAGGAGGAGGGCGAGTAGCGTGTCATGAGATCATGATGGCGACTCCTGCTATTCGAAACTTGATCC
GTGAAGATAAAGTGGCTCAGATGTATTCCATTATTCAAACGGGTGCCGCACATGGCATGCAGACCATGGAGCAAAATGCT
AAGCAACTACTGGCACAAGGCACGGTGGATCCGGAAGAGATATCGAAAAAAATTGAAACTGACTCTGGCCTATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

88.986

100

0.89
  pilT Vibrio cholerae strain A1552

88.986

100

0.89
  pilT Acinetobacter baumannii D1279779

71.687

96.232

0.69
  pilT Acinetobacter baumannii strain A118

71.687

96.232

0.69
  pilT Acinetobacter nosocomialis M2

71.386

96.232

0.687
  pilT Acinetobacter baylyi ADP1

71.515

95.652

0.684
  pilT Pseudomonas stutzeri DSM 10701

69.139

97.681

0.675
  pilT Pseudomonas aeruginosa PAK

67.953

97.681

0.664
  pilT Legionella pneumophila strain Lp02

68.997

95.362

0.658
  pilT Legionella pneumophila strain ERS1305867

68.997

95.362

0.658
  pilT Neisseria meningitidis 8013

63.478

100

0.635
  pilT Neisseria gonorrhoeae MS11

63.188

100

0.632
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.459

92.174

0.493
  pilU Vibrio cholerae strain A1552

42.515

96.812

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.916

96.812

0.406
  pilU Acinetobacter baylyi ADP1

40.625

92.754

0.377

Multiple sequence alignment