Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB2S62_RS12930 Genome accession   NZ_CP162100
Coordinates   2881449..2882555 (-) Length   368 a.a.
NCBI ID   WP_367987380.1    Uniprot ID   -
Organism   Vibrio sp. NTOU-M3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2876449..2887555
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB2S62_RS12900 (AB2S62_12655) - 2877220..2877720 (+) 501 WP_367987374.1 SprT family zinc-dependent metalloprotease -
  AB2S62_RS12905 (AB2S62_12660) endA 2877822..2878523 (+) 702 WP_367987375.1 deoxyribonuclease I -
  AB2S62_RS12910 (AB2S62_12665) rsmE 2878648..2879379 (+) 732 WP_367987376.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AB2S62_RS12915 (AB2S62_12670) gshB 2879393..2880340 (+) 948 WP_367987377.1 glutathione synthase -
  AB2S62_RS12920 (AB2S62_12675) - 2880382..2880945 (+) 564 WP_367987378.1 YqgE/AlgH family protein -
  AB2S62_RS12925 (AB2S62_12680) ruvX 2880962..2881387 (+) 426 WP_367987379.1 Holliday junction resolvase RuvX -
  AB2S62_RS12930 (AB2S62_12685) pilU 2881449..2882555 (-) 1107 WP_367987380.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB2S62_RS12935 (AB2S62_12690) pilT 2882568..2883605 (-) 1038 WP_367987381.1 type IV pilus twitching motility protein PilT Machinery gene
  AB2S62_RS12940 (AB2S62_12695) - 2883631..2884347 (+) 717 WP_367987382.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB2S62_RS12945 (AB2S62_12700) proC 2884370..2885188 (+) 819 WP_367987383.1 pyrroline-5-carboxylate reductase -
  AB2S62_RS12950 (AB2S62_12705) - 2885233..2885790 (+) 558 WP_367987384.1 YggT family protein -
  AB2S62_RS12955 (AB2S62_12710) yggU 2885790..2886080 (+) 291 WP_367987385.1 DUF167 family protein YggU -
  AB2S62_RS12960 (AB2S62_12715) - 2886123..2886554 (+) 432 WP_367987386.1 DUF4426 domain-containing protein -
  AB2S62_RS12965 (AB2S62_12720) - 2886584..2887180 (+) 597 WP_367987387.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41290.25 Da        Isoelectric Point: 6.8294

>NTDB_id=1024054 AB2S62_RS12930 WP_367987380.1 2881449..2882555(-) (pilU) [Vibrio sp. NTOU-M3]
MDINAFLQGMISQKASDLYITVGAPVLFRVDGELKAHGEKLSESDVAILLNSMMDDERSKEFHQTREANFAIVRDIGRFR
VSAFYQRELPGAVIRRIESNIPTFEQLRLPDVLQNLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKEQKEQFLFDLSMNLKGVVGQQLIRDKHGKGRHGVFEILLNSPRVSDLIRKGDLHELKSTMSKSNEIGMQTFDQALY
QLVVDGKINEEDALHSADSANDLRLMLKTKRGDSYSSGKLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1024054 AB2S62_RS12930 WP_367987380.1 2881449..2882555(-) (pilU) [Vibrio sp. NTOU-M3]
ATGGACATAAATGCTTTTTTACAGGGAATGATCAGCCAGAAGGCGTCGGATCTTTATATTACAGTTGGTGCTCCAGTACT
GTTTAGGGTCGATGGTGAGCTAAAAGCTCATGGGGAGAAATTATCTGAATCTGATGTGGCGATTTTGCTGAACTCAATGA
TGGATGATGAACGCAGTAAAGAGTTTCATCAGACGCGAGAAGCGAACTTCGCCATCGTTAGAGATATTGGCCGTTTTCGT
GTGAGTGCGTTCTATCAGCGTGAGCTGCCGGGCGCGGTGATTCGTCGTATTGAGTCTAATATCCCGACCTTTGAACAACT
GAGACTGCCTGATGTGCTGCAGAATTTAGCGATTGCCAAGCGTGGTTTAGTGTTGGTGGTTGGTGCTACGGGGTCAGGTA
AGTCGACCACAATGGCAGCGATGACGGGTTATCGCAATACGCACCGAACTGGCCATATTCTTACGGTGGAAGATCCGATT
GAATTTGTGCACGAGCATAAGCGCTGCATTGTGACTCAGCGTGAAGTCGGGTTGGATACCGAAAGCTATGAAGTTGCACT
GAAAAACTCATTACGCCAAGCTCCAGATATGATCTTAATTGGTGAAATCCGTAGTCGAGAAACGATGGAATATGCCATGA
ACTTTGCCGAAACAGGGCACTTGTGTATGGCTACGTTGCACGCAAATAATGCGAACCAAGCGTTAGAGCGTATTTTGCAT
CTGGTACCTAAGGAGCAAAAAGAGCAGTTCTTATTTGATTTATCGATGAACCTGAAAGGGGTGGTTGGTCAGCAGTTGAT
CCGTGATAAACATGGTAAAGGTCGACATGGGGTGTTTGAGATTCTGCTCAATAGCCCGCGTGTTTCTGATTTGATCCGTA
AGGGCGATTTGCACGAGTTGAAGTCGACGATGTCGAAGTCCAATGAAATTGGTATGCAGACGTTTGATCAAGCGCTTTAT
CAGTTGGTGGTTGATGGGAAGATCAATGAAGAAGACGCATTGCATAGTGCCGATTCCGCAAACGATCTTCGTTTGATGCT
CAAGACTAAACGTGGTGATAGCTATTCGTCAGGGAAATTAGCGAATGTGAAGATCGACATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

84.239

100

0.842
  pilU Pseudomonas stutzeri DSM 10701

58.857

95.109

0.56
  pilU Acinetobacter baylyi ADP1

53.561

95.38

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.062

88.043

0.397
  pilT Legionella pneumophila strain Lp02

43.068

92.12

0.397
  pilT Legionella pneumophila strain ERS1305867

43.068

92.12

0.397
  pilT Acinetobacter baylyi ADP1

42.229

92.663

0.391
  pilT Pseudomonas aeruginosa PAK

42.183

92.12

0.389
  pilT Acinetobacter baumannii strain A118

41.003

92.12

0.378
  pilT Pseudomonas stutzeri DSM 10701

41.003

92.12

0.378
  pilT Acinetobacter baumannii D1279779

41.003

92.12

0.378
  pilT Acinetobacter nosocomialis M2

41.003

92.12

0.378
  pilT Vibrio cholerae strain A1552

41.018

90.761

0.372
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.018

90.761

0.372
  pilT Neisseria gonorrhoeae MS11

39.42

93.75

0.37
  pilT Neisseria meningitidis 8013

39.42

93.75

0.37

Multiple sequence alignment