Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB3G31_RS20840 Genome accession   NZ_CP162006
Coordinates   4476375..4477418 (+) Length   347 a.a.
NCBI ID   WP_367847954.1    Uniprot ID   -
Organism   Rhodoferax sp. WC2427     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4471375..4482418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3G31_RS20810 (AB3G31_20810) - 4472298..4472723 (+) 426 WP_367847950.1 Lrp/AsnC family transcriptional regulator -
  AB3G31_RS20815 (AB3G31_20815) - 4472781..4473221 (+) 441 WP_367850392.1 Lrp/AsnC ligand binding domain-containing protein -
  AB3G31_RS20820 (AB3G31_20820) - 4473218..4473793 (+) 576 WP_367847951.1 YfiR family protein -
  AB3G31_RS20825 (AB3G31_20825) - 4473790..4475088 (+) 1299 WP_367847952.1 diguanylate cyclase -
  AB3G31_RS20830 (AB3G31_20830) - 4475088..4475624 (+) 537 WP_367847953.1 OmpA family protein -
  AB3G31_RS20835 (AB3G31_20835) - 4475645..4476337 (-) 693 WP_367850393.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB3G31_RS20840 (AB3G31_20840) pilT 4476375..4477418 (+) 1044 WP_367847954.1 type IV pilus twitching motility protein PilT Machinery gene
  AB3G31_RS20845 (AB3G31_20845) - 4477447..4478079 (+) 633 WP_367847955.1 cyclic nucleotide-binding domain-containing protein -
  AB3G31_RS20850 (AB3G31_20850) pilU 4478090..4479226 (+) 1137 WP_367847956.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB3G31_RS20855 (AB3G31_20855) - 4479297..4480211 (+) 915 WP_367847957.1 NAD(P)-dependent oxidoreductase -
  AB3G31_RS20860 (AB3G31_20860) - 4480219..4480827 (-) 609 WP_367847958.1 BON domain-containing protein -
  AB3G31_RS20865 (AB3G31_20865) - 4480844..4481443 (-) 600 WP_348007188.1 phosphoheptose isomerase -
  AB3G31_RS20870 (AB3G31_20870) - 4481492..4481884 (-) 393 WP_367847959.1 YraN family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38128.86 Da        Isoelectric Point: 6.9356

>NTDB_id=1023460 AB3G31_RS20840 WP_367847954.1 4476375..4477418(+) (pilT) [Rhodoferax sp. WC2427]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKTVHAMVYDIMSDSQRKQYEEFLEVDFSFEIEGLAR
FRVNAFNHARGAGAVLRTIPSKILTLEQLNAPKIFGDLALKPRGMVLVTGPTGSGKSTTLAAMVNHLNETEYGHVLTVED
PIEFVHESKKCLINQREVGPHTLSFAAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTGNSVGMQTLDQN
LTDLVRRNIISPAEARGKAKIPENFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1023460 AB3G31_RS20840 WP_367847954.1 4476375..4477418(+) (pilT) [Rhodoferax sp. WC2427]
GTGGACATCACCCAACTTCTCGCATTCAGCGTAAAAAACAAGGCCTCGGATTTGCACCTCTCGGCGGGGTTGCCCCCCAT
GATCCGGGTGCATGGCGATGTGCGCCGCATCAACGTCGATCCGCTGGACCACAAAACCGTCCATGCCATGGTCTACGACA
TCATGAGCGACAGCCAACGCAAACAGTACGAAGAGTTTTTAGAGGTCGATTTCTCGTTCGAAATTGAAGGACTGGCGCGG
TTTCGGGTCAACGCCTTCAACCACGCACGCGGTGCCGGTGCCGTGCTGCGGACCATTCCCTCCAAGATTCTCACGCTGGA
GCAGCTCAACGCGCCCAAGATCTTTGGCGACCTGGCCCTCAAACCGCGGGGCATGGTGTTGGTGACCGGGCCCACCGGTT
CCGGCAAATCCACCACCCTGGCGGCCATGGTGAACCACCTCAACGAAACCGAATACGGCCATGTGCTGACGGTGGAAGAC
CCGATCGAGTTTGTCCACGAATCCAAAAAATGCCTGATTAACCAGCGCGAAGTGGGCCCGCACACGCTGAGCTTTGCTGC
CGCCCTGCGCTCGGCCCTGCGCGAAGACCCGGATGCGATCCTGGTGGGTGAAATGCGCGACCTGGAAACCATCCGCCTGG
CCATGACGGCGGCCGAAACCGGGCATTTGGTGTTTGGCACCCTGCACACCTCCAGCGCGGCCAAAACCATTGACCGGATC
ATTGACGTGTTCCCGGCAGAAGAAAAAGAAATGGTCCGCGCCATGCTGTCGGAGTCGCTACAGGCCGTCATCTCGCAAAC
CCTGTGTAAAACCAAGGATGGCCAGGGCCGGGTGGCTGCGCACGAAATCATGCTGGGCACCAGCGCCATCCGTAACCTGA
TCCGTGAGGCCAAGGTGGCGCAGATGTATTCCACCATCCAGACCGGCAACAGTGTGGGGATGCAGACCCTCGACCAAAAC
CTGACCGATTTGGTACGCCGCAACATCATCAGCCCGGCCGAAGCCCGGGGCAAAGCCAAAATCCCGGAGAATTTCCCGGG
ATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

75.652

99.424

0.752
  pilT Acinetobacter baumannii D1279779

75.652

99.424

0.752
  pilT Acinetobacter baumannii strain A118

75.652

99.424

0.752
  pilT Pseudomonas stutzeri DSM 10701

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

74.203

99.424

0.738
  pilT Legionella pneumophila strain Lp02

72.861

97.695

0.712
  pilT Legionella pneumophila strain ERS1305867

72.861

97.695

0.712
  pilT Neisseria meningitidis 8013

68.116

99.424

0.677
  pilT Neisseria gonorrhoeae MS11

67.826

99.424

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.276

98.271

0.651
  pilT Vibrio cholerae strain A1552

66.276

98.271

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

99.424

0.507
  pilU Vibrio cholerae strain A1552

42.388

96.542

0.409
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

40.896

96.542

0.395

Multiple sequence alignment