Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB3G31_RS20850 Genome accession   NZ_CP162006
Coordinates   4478090..4479226 (+) Length   378 a.a.
NCBI ID   WP_367847956.1    Uniprot ID   -
Organism   Rhodoferax sp. WC2427     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4473090..4484226
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB3G31_RS20820 (AB3G31_20820) - 4473218..4473793 (+) 576 WP_367847951.1 YfiR family protein -
  AB3G31_RS20825 (AB3G31_20825) - 4473790..4475088 (+) 1299 WP_367847952.1 diguanylate cyclase -
  AB3G31_RS20830 (AB3G31_20830) - 4475088..4475624 (+) 537 WP_367847953.1 OmpA family protein -
  AB3G31_RS20835 (AB3G31_20835) - 4475645..4476337 (-) 693 WP_367850393.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB3G31_RS20840 (AB3G31_20840) pilT 4476375..4477418 (+) 1044 WP_367847954.1 type IV pilus twitching motility protein PilT Machinery gene
  AB3G31_RS20845 (AB3G31_20845) - 4477447..4478079 (+) 633 WP_367847955.1 cyclic nucleotide-binding domain-containing protein -
  AB3G31_RS20850 (AB3G31_20850) pilU 4478090..4479226 (+) 1137 WP_367847956.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB3G31_RS20855 (AB3G31_20855) - 4479297..4480211 (+) 915 WP_367847957.1 NAD(P)-dependent oxidoreductase -
  AB3G31_RS20860 (AB3G31_20860) - 4480219..4480827 (-) 609 WP_367847958.1 BON domain-containing protein -
  AB3G31_RS20865 (AB3G31_20865) - 4480844..4481443 (-) 600 WP_348007188.1 phosphoheptose isomerase -
  AB3G31_RS20870 (AB3G31_20870) - 4481492..4481884 (-) 393 WP_367847959.1 YraN family protein -
  AB3G31_RS20875 (AB3G31_20875) rsmI 4481884..4482798 (+) 915 WP_367847960.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41789.09 Da        Isoelectric Point: 7.1861

>NTDB_id=1023461 AB3G31_RS20850 WP_367847956.1 4478090..4479226(+) (pilU) [Rhodoferax sp. WC2427]
MERDQASKFINDLLKLMVSRNGSDLFITSEFPPGIKVDGKVTKVSPQPLNAAHTLALARSVMNDKQAAEFERTKECNFAI
SPAGIGRFRVNAFVQQGKVGMVLRVIPVSIPSIDGLAMPQILKDVAMTKRGLCILVGGTGCGKSTTLAAMVDWRNENSYG
HIITIEDPVEFVHAHKNCVVTQREVGLDTDSWDSALKNSLRQAPDVILMGEIRDRTTMEHAIAFAETGHLCLATLHANNA
NQALDRILNFFPEDRRAQLLMDLSLNLRGVVSQRLIPRQDGKGRAAAVEILLNTPLISDLIFKGEVTEIKEIMKKSRNLG
MQTFDQALFDAYESQLISYEDALRHADSLNDLRLQIKLNSQRAKTQDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1023461 AB3G31_RS20850 WP_367847956.1 4478090..4479226(+) (pilU) [Rhodoferax sp. WC2427]
ATGGAACGCGACCAAGCCAGCAAGTTCATCAATGACCTGTTGAAACTCATGGTCAGCCGCAATGGCAGCGACCTGTTCAT
CACATCCGAGTTTCCGCCCGGCATCAAGGTCGATGGCAAGGTCACCAAAGTCTCCCCCCAGCCGCTCAACGCTGCCCACA
CGCTGGCCCTGGCCCGTTCGGTGATGAACGACAAACAGGCCGCCGAGTTTGAGCGCACCAAGGAATGCAACTTTGCGATC
TCTCCCGCGGGCATAGGCCGTTTTCGCGTGAATGCCTTTGTGCAGCAGGGCAAGGTGGGCATGGTGCTGCGGGTCATTCC
GGTCAGCATCCCCTCGATCGATGGCCTGGCCATGCCGCAGATCCTGAAAGATGTGGCCATGACCAAGCGGGGTCTGTGCA
TCCTGGTCGGCGGCACCGGCTGCGGCAAGTCCACCACCCTGGCGGCCATGGTCGATTGGCGCAACGAAAACTCCTACGGC
CACATCATCACCATCGAAGACCCGGTGGAGTTTGTGCATGCGCACAAAAACTGCGTGGTCACCCAACGCGAAGTGGGCCT
GGACACCGACAGCTGGGATTCCGCCCTGAAGAATTCGCTGCGCCAGGCCCCTGACGTCATTCTGATGGGTGAAATCCGCG
ACCGCACCACCATGGAGCACGCCATCGCCTTTGCCGAAACCGGCCACCTGTGCCTGGCGACACTGCATGCCAACAACGCC
AACCAGGCGCTGGACCGCATCTTGAACTTTTTCCCCGAAGACCGCCGCGCCCAGTTGCTGATGGATTTGTCGCTGAACCT
GCGGGGCGTGGTGTCGCAGCGCCTGATCCCCCGGCAGGACGGCAAGGGCCGTGCCGCAGCGGTGGAGATTTTGCTCAACA
CCCCGCTGATCTCCGACCTGATCTTCAAAGGCGAGGTCACCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCCCTGTTCGACGCCTACGAGAGCCAGCTCATCAGCTACGAAGATGCCCTGCGCCACGCCGA
CTCGCTCAACGACCTGCGTTTGCAGATCAAGCTCAACAGCCAGCGCGCTAAAACACAAGATTTAGCGGCCGGGACCGAGC
ATTTCGCGATCGTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

58.774

94.974

0.558
  pilU Vibrio cholerae strain A1552

54.023

92.063

0.497
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.62

89.153

0.389
  pilT Pseudomonas aeruginosa PAK

43.027

89.153

0.384
  pilT Legionella pneumophila strain Lp02

41.642

90.212

0.376
  pilT Legionella pneumophila strain ERS1305867

41.642

90.212

0.376
  pilT Pseudomonas stutzeri DSM 10701

41.246

89.153

0.368

Multiple sequence alignment