Detailed information NTG1021960

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: AB1X99_RS20975
• Genome accession: NZ_CP160649
• Coordinates: 4041968..4042948 (+)
• Length: 326 a.a.
• NCBI ID: WP_071844792.1
• Uniprot ID: -
• Organism: Shigella flexneri strain 100537
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4036968..4047948

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB1X99_RS20945 (AB1X99_20975)	-	4037483..4038490 (+)	1008	WP_000745224.1	DUF1202 family protein	-
AB1X99_RS20950 (AB1X99_20980)	hemW	4038645..4039781 (-)	1137	WP_000239922.1	radical SAM family heme chaperone HemW	-
AB1X99_RS20955 (AB1X99_20985)	-	4039774..4040367 (-)	594	WP_050902028.1	XTP/dITP diphosphatase	-
AB1X99_RS20960 (AB1X99_20990)	yggU	4040375..4040665 (-)	291	WP_000994920.1	DUF167 family protein YggU	-
AB1X99_RS20965 (AB1X99_20995)	yggT	4040662..4041228 (-)	567	WP_001094829.1	osmotic shock tolerance protein YggT	-
AB1X99_RS20970 (AB1X99_21000)	yggS	4041246..4041950 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
AB1X99_RS20975 (AB1X99_21005)	pilT	4041968..4042948 (+)	981	WP_071844792.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB1X99_RS20980 (AB1X99_21010)	ruvX	4043124..4043540 (-)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
AB1X99_RS20985 (AB1X99_21015)	-	4043540..4044103 (-)	564	WP_004986431.1	YqgE/AlgH family protein	-
AB1X99_RS20990 (AB1X99_21020)	gshB	4044212..4045162 (-)	951	WP_000593283.1	glutathione synthase	-
AB1X99_RS20995 (AB1X99_21025)	rsmE	4045175..4045906 (-)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
AB1X99_RS21000 (AB1X99_21030)	endA	4045986..4046693 (-)	708	WP_000286500.1	deoxyribonuclease I	-
AB1X99_RS21005 (AB1X99_21035)	-	4046788..4047285 (-)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35928.14 Da
• Isoelectric Point: 5.7654

>NTDB_id=1021960 AB1X99_RS20975 WP_071844792.1 4041968..4042948(+) (pilT) [Shigella flexneri strain 100537]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDALDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1021960 AB1X99_RS20975 WP_071844792.1 4041968..4042948(+) (pilT) [Shigella flexneri strain 100537]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCTGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTAGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter nosocomialis M2	46.789	100	0.469
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter baumannii D1279779	46.789	100	0.469
pilT	Acinetobacter baumannii strain A118	46.789	100	0.469
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	38.75	98.16	0.38
pilU	Acinetobacter baylyi ADP1	36.957	98.773	0.365
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365