Detailed information NTG1021914

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: AB1Y00_RS21070
• Genome accession: NZ_CP160646
• Coordinates: 3823623..3824603 (-)
• Length: 326 a.a.
• NCBI ID: WP_071844792.1
• Uniprot ID: -
• Organism: Shigella flexneri strain 403127
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3818623..3829603

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AB1Y00_RS21040 (AB1Y00_21090)	-	3819286..3819783 (+)	498	WP_001300769.1	SprT family zinc-dependent metalloprotease	-
AB1Y00_RS21045 (AB1Y00_21095)	endA	3819878..3820585 (+)	708	WP_000286500.1	deoxyribonuclease I	-
AB1Y00_RS21050 (AB1Y00_21100)	rsmE	3820665..3821396 (+)	732	WP_001222508.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
AB1Y00_RS21055 (AB1Y00_21105)	gshB	3821409..3822359 (+)	951	WP_000593283.1	glutathione synthase	-
AB1Y00_RS21060 (AB1Y00_21110)	-	3822468..3823031 (+)	564	WP_004986431.1	YqgE/AlgH family protein	-
AB1Y00_RS21065 (AB1Y00_21115)	ruvX	3823031..3823447 (+)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
AB1Y00_RS21070 (AB1Y00_21120)	pilT	3823623..3824603 (-)	981	WP_071844792.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
AB1Y00_RS21075 (AB1Y00_21125)	yggS	3824621..3825325 (+)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
AB1Y00_RS21080 (AB1Y00_21130)	yggT	3825343..3825909 (+)	567	WP_001094829.1	osmotic shock tolerance protein YggT	-
AB1Y00_RS21085 (AB1Y00_21135)	yggU	3825906..3826196 (+)	291	WP_000994920.1	DUF167 family protein YggU	-
AB1Y00_RS21090 (AB1Y00_21140)	-	3826204..3826797 (+)	594	WP_001174735.1	XTP/dITP diphosphatase	-
AB1Y00_RS21095 (AB1Y00_21145)	hemW	3826790..3827926 (+)	1137	WP_000239922.1	radical SAM family heme chaperone HemW	-
AB1Y00_RS21100 (AB1Y00_21150)	-	3828081..3829088 (-)	1008	WP_000745224.1	DUF1202 family protein	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35928.14 Da
• Isoelectric Point: 5.7654

>NTDB_id=1021914 AB1Y00_RS21070 WP_071844792.1 3823623..3824603(-) (pilT) [Shigella flexneri strain 403127]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDALDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRHGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1021914 AB1Y00_RS21070 WP_071844792.1 3823623..3824603(-) (pilT) [Shigella flexneri strain 403127]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCTGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCATGGCATTTCGCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATATCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTAGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter nosocomialis M2	46.789	100	0.469
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter baumannii D1279779	46.789	100	0.469
pilT	Acinetobacter baumannii strain A118	46.789	100	0.469
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	45.872	100	0.46
pilT	Legionella pneumophila strain ERS1305867	44.954	100	0.451
pilT	Legionella pneumophila strain Lp02	44.954	100	0.451
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.694	97.239	0.396
pilU	Pseudomonas stutzeri DSM 10701	38.75	98.16	0.38
pilU	Acinetobacter baylyi ADP1	36.957	98.773	0.365
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365