Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB1723_RS00850 Genome accession   NZ_CP160633
Coordinates   159398..160435 (+) Length   345 a.a.
NCBI ID   WP_004147099.1    Uniprot ID   A0AAP7J2J0
Organism   Stenotrophomonas cyclobalanopsidis strain Ed1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 154398..165435
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB1723_RS00825 (AB1723_00825) soxR 155694..156128 (+) 435 WP_367381564.1 redox-sensitive transcriptional activator SoxR -
  AB1723_RS00830 (AB1723_00830) - 156175..156777 (-) 603 WP_367381565.1 short chain dehydrogenase -
  AB1723_RS00835 (AB1723_00835) - 156874..157746 (+) 873 WP_367381566.1 LysR family transcriptional regulator -
  AB1723_RS00840 (AB1723_00840) proC 157796..158617 (-) 822 WP_367381567.1 pyrroline-5-carboxylate reductase -
  AB1723_RS00845 (AB1723_00845) - 158633..159310 (-) 678 WP_367381568.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB1723_RS00850 (AB1723_00850) pilT 159398..160435 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  AB1723_RS00855 (AB1723_00855) pilU 160532..161683 (+) 1152 WP_367381569.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB1723_RS00860 (AB1723_00860) - 161703..162398 (+) 696 WP_367381570.1 PIN domain-containing protein -
  AB1723_RS00865 (AB1723_00865) - 162597..163202 (+) 606 WP_367381571.1 hypothetical protein -
  AB1723_RS00870 (AB1723_00870) - 163306..164289 (+) 984 WP_367381572.1 aldo/keto reductase -
  AB1723_RS00875 (AB1723_00875) - 164354..165253 (-) 900 WP_367381573.1 LysR substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38658.41 Da        Isoelectric Point: 6.6654

>NTDB_id=1021722 AB1723_RS00850 WP_004147099.1 159398..160435(+) (pilT) [Stenotrophomonas cyclobalanopsidis strain Ed1]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1021722 AB1723_RS00850 WP_004147099.1 159398..160435(+) (pilT) [Stenotrophomonas cyclobalanopsidis strain Ed1]
ATGGATATCGCCGAGCTGTTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTGGACGGCGATGTTCGCCGGATCAACATCCCGGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGGCGCGATTACGAGGAATTCCTCGAAGTCGACTTCTCCTTCGAGATTCCATCGCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTATTCCGTACCATTCCGTCCGAGGTGCTCACTCTGGA
GGATCTGGCCTGCCCGCCGCTGTTCCGCGAAGTGATCCAGCAGCCGCAGGGGTTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACTGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
AGCACTGCGCTCGGCACTGCGTGAGGATCCGGACATCATCCTGGTGGGCGAACTCCGCGACCTGGAAACGATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACTCTGCATACCAGCTCGGCGGCGAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTAATCTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGTACCGCGGCGTGGGAAATCATGGTCGGCACACCGGCCATCCGCAATCTGATCC
GCGAAGACAAGGTTGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGTACGGGATGATGACCCTTGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACCCGCAACCAGGCCCGCGAGTACGCCAAGGACAAGCGTCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391

Multiple sequence alignment