Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB1723_RS00855 Genome accession   NZ_CP160633
Coordinates   160532..161683 (+) Length   383 a.a.
NCBI ID   WP_367381569.1    Uniprot ID   -
Organism   Stenotrophomonas cyclobalanopsidis strain Ed1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 155532..166683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB1723_RS00825 (AB1723_00825) soxR 155694..156128 (+) 435 WP_367381564.1 redox-sensitive transcriptional activator SoxR -
  AB1723_RS00830 (AB1723_00830) - 156175..156777 (-) 603 WP_367381565.1 short chain dehydrogenase -
  AB1723_RS00835 (AB1723_00835) - 156874..157746 (+) 873 WP_367381566.1 LysR family transcriptional regulator -
  AB1723_RS00840 (AB1723_00840) proC 157796..158617 (-) 822 WP_367381567.1 pyrroline-5-carboxylate reductase -
  AB1723_RS00845 (AB1723_00845) - 158633..159310 (-) 678 WP_367381568.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB1723_RS00850 (AB1723_00850) pilT 159398..160435 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  AB1723_RS00855 (AB1723_00855) pilU 160532..161683 (+) 1152 WP_367381569.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB1723_RS00860 (AB1723_00860) - 161703..162398 (+) 696 WP_367381570.1 PIN domain-containing protein -
  AB1723_RS00865 (AB1723_00865) - 162597..163202 (+) 606 WP_367381571.1 hypothetical protein -
  AB1723_RS00870 (AB1723_00870) - 163306..164289 (+) 984 WP_367381572.1 aldo/keto reductase -
  AB1723_RS00875 (AB1723_00875) - 164354..165253 (-) 900 WP_367381573.1 LysR substrate-binding domain-containing protein -
  AB1723_RS00880 (AB1723_00880) - 165654..165866 (+) 213 WP_367381574.1 DUF4287 domain-containing protein -

Sequence

Protein


Download         Length: 383 a.a.        Molecular weight: 42613.90 Da        Isoelectric Point: 6.5902

>NTDB_id=1021723 AB1723_RS00855 WP_367381569.1 160532..161683(+) (pilU) [Stenotrophomonas cyclobalanopsidis strain Ed1]
MNSTASTTSSIDFTSFLKLMAHQRASDLFITAGMPPAIKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECN
FAIGLSGVGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQN
STGHIITIEDPIEFVHKHEGCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHA
NNANQAMDRIINFFPEDRRNQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKELMKESV
ELGMKTFDQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEVR

Nucleotide


Download         Length: 1152 bp        

>NTDB_id=1021723 AB1723_RS00855 WP_367381569.1 160532..161683(+) (pilU) [Stenotrophomonas cyclobalanopsidis strain Ed1]
ATGAACAGCACCGCCAGTACCACCAGCTCCATCGATTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGCGCGCCTCGGA
CCTGTTCATCACCGCGGGCATGCCGCCGGCGATCAAGGTCAACGGCAAGATCTCGCCGATCACGCAGACGCCGCTGACCC
CGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACTCCGGCGCAGCGCGAGGAATTCGAGAAGACCCACGAGTGCAAC
TTCGCCATCGGCCTGTCTGGTGTCGGTCGTTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCG
TCGTATCGAAACGCGCATCCCGACCGTGGAAGAACTGAGCCTGCCGCCGATCATCAAGACGCTGGCGATGACCAAGCGCG
GCATCATCCTCTTCGTTGGCGCCACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAAC
TCGACCGGCCATATCATCACCATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGA
AGTCGGCATCGATACCGACAGCTGGGAAGCCGCCCTGAAGAACACCCTGCGCCAGGCACCGGACGTGATCATGATCGGCG
AGGTGCGTACCCGGGAGGGCATGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCATGCC
AACAACGCCAACCAGGCGATGGACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCTGATGGATCTGTC
GCTGAACCTGAAGGGCGTGGTCGCGCAGCAGCTTGTTCCTTCGCCCGACGGCCGTTCGCGCAAGGTCGCGATGGAGATCC
TGCTGGGCACGCCGCTGGTGCAGGACTATATCCGCGACGGCGAGATCCACAAGCTGAAGGAGCTGATGAAGGAGTCCGTC
GAACTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAGATCAGCTACGAGGACGCGCTGCG
TTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAAGGCGGCGACGCACGCACGCTGTCGCAGGGTC
TGGATGGGGTGGAGATTTCCGAGGTGCGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-280)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.12

95.822

0.653
  pilU Acinetobacter baylyi ADP1

64.426

93.211

0.601
  pilU Vibrio cholerae strain A1552

53.652

92.95

0.499
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.737

93.473

0.399
  pilT Acinetobacter nosocomialis M2

40.116

89.817

0.36
  pilT Acinetobacter baumannii D1279779

40.116

89.817

0.36
  pilT Acinetobacter baumannii strain A118

40.116

89.817

0.36

Multiple sequence alignment