Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB1S68_RS02470 Genome accession   NZ_CP160473
Coordinates   565314..566348 (+) Length   344 a.a.
NCBI ID   WP_011470110.1    Uniprot ID   Q21EI4
Organism   Saccharophagus degradans strain HMH0024     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 560314..571348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB1S68_RS02440 (AB1S68_02435) - 560751..561191 (-) 441 WP_216063513.1 DUF4426 domain-containing protein -
  AB1S68_RS02445 (AB1S68_02440) metW 561184..561780 (-) 597 WP_011470115.1 methionine biosynthesis protein MetW -
  AB1S68_RS02450 (AB1S68_02445) metX 561782..562927 (-) 1146 WP_216063512.1 homoserine O-succinyltransferase MetX -
  AB1S68_RS02455 (AB1S68_02450) - 562961..563551 (-) 591 WP_280947019.1 YggT family protein -
  AB1S68_RS02460 (AB1S68_02455) proC 563560..564417 (-) 858 WP_280947020.1 pyrroline-5-carboxylate reductase -
  AB1S68_RS02465 (AB1S68_02460) - 564457..565137 (-) 681 WP_280947021.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB1S68_RS02470 (AB1S68_02465) pilT 565314..566348 (+) 1035 WP_011470110.1 type IV pilus twitching motility protein PilT Machinery gene
  AB1S68_RS02475 (AB1S68_02470) pilU 566383..567525 (+) 1143 WP_011470109.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB1S68_RS02480 (AB1S68_02475) ruvX 567549..567977 (-) 429 WP_011470108.1 Holliday junction resolvase RuvX -
  AB1S68_RS02485 (AB1S68_02480) - 568033..568644 (-) 612 WP_216063508.1 YqgE/AlgH family protein -
  AB1S68_RS02490 (AB1S68_02485) - 568733..569617 (-) 885 WP_280947022.1 energy transducer TonB -
  AB1S68_RS02495 (AB1S68_02490) gshB 569637..570560 (-) 924 WP_416456070.1 glutathione synthase -
  AB1S68_RS02500 (AB1S68_02495) pilG 570851..571246 (+) 396 WP_011470104.1 twitching motility response regulator PilG Regulator

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38154.75 Da        Isoelectric Point: 6.5785

>NTDB_id=1021431 AB1S68_RS02470 WP_011470110.1 565314..566348(+) (pilT) [Saccharophagus degradans strain HMH0024]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPMEHKQVHGLIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGSGAVFRTIPSKVLTMEELGMGQVFRDVSSVPRGLVLVTGPTGSGKSTTLAAMIDYINDNKYHHILTIED
PIEFVHESKKCLVNQREVHRDTHGFAEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPANEKAMVRSMLSESLEAVVSQTLLKKNGGGRVAAHEIMRGTSAIRNLIREDKVAQMYSAIQTGSAVGMQTMDQCL
ADLVERRIISRDVAKEKAKMPDQF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1021431 AB1S68_RS02470 WP_011470110.1 565314..566348(+) (pilT) [Saccharophagus degradans strain HMH0024]
ATGGATATTACTGAACTCCTCGCTTTCTCTGCCAAGCAGGGAGCATCGGATTTACACCTTTCGGCGGGCTTGCCGCCCAT
GATTCGTGTGGATGGCGATGTACGCCGCATTAACTTGCCGCCGATGGAGCACAAGCAAGTCCACGGGCTAATTTACGACA
TTATGAACGACAAGCAGCGCAAGGATTTCGAAGAATTCCTCGAGACCGACTTTTCGTTTGAAGTGCCCGGTGTGGCGCGC
TTCCGTGTTAACGCCTTTAACCAAAACCGTGGCTCTGGTGCGGTGTTTCGTACCATTCCTTCCAAAGTACTCACCATGGA
AGAATTGGGTATGGGGCAGGTGTTCCGCGATGTATCTTCGGTGCCGCGCGGCTTGGTGTTGGTAACCGGGCCGACCGGTT
CGGGTAAGTCCACCACCCTTGCGGCCATGATCGATTATATTAACGACAACAAATACCACCATATTCTTACCATCGAAGAC
CCGATCGAATTCGTGCACGAAAGTAAGAAGTGCCTAGTAAACCAGCGTGAAGTGCACCGCGATACCCACGGCTTCGCTGA
AGCACTGCGCTCGGCACTGCGGGAAGACCCCGATATTATTCTGGTGGGTGAGCTACGTGACTTGGAAACCATTCGCTTGG
CACTAACCGCGGCGGAAACCGGTCACTTGGTGTTCGGCACCTTGCACACCACCTCGGCGGCCAAAACCATTGACCGGGTA
GTGGATGTATTTCCCGCCAACGAGAAAGCCATGGTGCGTTCTATGTTGTCGGAATCCCTCGAAGCGGTTGTTTCGCAAAC
ACTTTTGAAGAAAAACGGCGGTGGCCGTGTAGCAGCGCACGAAATTATGCGCGGCACCTCAGCAATTCGTAACTTAATCC
GCGAAGATAAGGTTGCGCAAATGTATTCTGCTATTCAAACCGGTAGTGCGGTGGGTATGCAAACAATGGATCAGTGTTTG
GCCGATCTTGTTGAGCGCCGCATTATTAGTCGCGATGTAGCGAAAGAAAAAGCAAAAATGCCCGATCAGTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q21EI4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

88.663

100

0.887
  pilT Pseudomonas stutzeri DSM 10701

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

81.977

100

0.82
  pilT Acinetobacter baumannii D1279779

81.977

100

0.82
  pilT Acinetobacter baumannii strain A118

81.977

100

0.82
  pilT Acinetobacter baylyi ADP1

78.779

100

0.788
  pilT Legionella pneumophila strain Lp02

75.444

98.256

0.741
  pilT Legionella pneumophila strain ERS1305867

75.444

98.256

0.741
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.353

98.837

0.666
  pilT Vibrio cholerae strain A1552

67.353

98.837

0.666
  pilT Neisseria meningitidis 8013

67.353

98.837

0.666
  pilT Neisseria gonorrhoeae MS11

66.765

98.837

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Vibrio cholerae strain A1552

41.231

94.477

0.39
  pilU Pseudomonas stutzeri DSM 10701

41.358

94.186

0.39
  pilU Acinetobacter baylyi ADP1

37.861

100

0.381

Multiple sequence alignment