Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB1S68_RS02475 Genome accession   NZ_CP160473
Coordinates   566383..567525 (+) Length   380 a.a.
NCBI ID   WP_011470109.1    Uniprot ID   Q21EI5
Organism   Saccharophagus degradans strain HMH0024     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 561383..572525
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB1S68_RS02450 (AB1S68_02445) metX 561782..562927 (-) 1146 WP_216063512.1 homoserine O-succinyltransferase MetX -
  AB1S68_RS02455 (AB1S68_02450) - 562961..563551 (-) 591 WP_280947019.1 YggT family protein -
  AB1S68_RS02460 (AB1S68_02455) proC 563560..564417 (-) 858 WP_280947020.1 pyrroline-5-carboxylate reductase -
  AB1S68_RS02465 (AB1S68_02460) - 564457..565137 (-) 681 WP_280947021.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB1S68_RS02470 (AB1S68_02465) pilT 565314..566348 (+) 1035 WP_011470110.1 type IV pilus twitching motility protein PilT Machinery gene
  AB1S68_RS02475 (AB1S68_02470) pilU 566383..567525 (+) 1143 WP_011470109.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB1S68_RS02480 (AB1S68_02475) ruvX 567549..567977 (-) 429 WP_011470108.1 Holliday junction resolvase RuvX -
  AB1S68_RS02485 (AB1S68_02480) - 568033..568644 (-) 612 WP_216063508.1 YqgE/AlgH family protein -
  AB1S68_RS02490 (AB1S68_02485) - 568733..569617 (-) 885 WP_280947022.1 energy transducer TonB -
  AB1S68_RS02495 (AB1S68_02490) gshB 569637..570560 (-) 924 WP_416456070.1 glutathione synthase -
  AB1S68_RS02500 (AB1S68_02495) pilG 570851..571246 (+) 396 WP_011470104.1 twitching motility response regulator PilG Regulator
  AB1S68_RS02505 (AB1S68_02500) pilH 571300..571662 (+) 363 WP_011470103.1 twitching motility response regulator PilH -
  AB1S68_RS02510 (AB1S68_02505) - 571713..572252 (+) 540 WP_216063505.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 380 a.a.        Molecular weight: 42469.57 Da        Isoelectric Point: 6.0959

>NTDB_id=1021432 AB1S68_RS02475 WP_011470109.1 566383..567525(+) (pilU) [Saccharophagus degradans strain HMH0024]
MELDRLLSLMVDKQASDLFITAGVAPSIKLHGKIVPVSTTPLSPEKSRELVLSVMNEKQRKEFLEAKELNFAVSARGVGR
FRASAFYQRNLAGMVLRRIETNIPQIDELGLPEIIKELAMIKRGLVMFVGATGTGKSTSLAAMIGHRNRNSKGHIISIED
PIEFIHQHQGCIVTQREVGIDTDSFEVALKNTLRQAPDVILIGEVRSRETMDHAIAFAETGHLCLCTLHANNANQALDRI
IHFFPADRHRQLWMDLSLNLKGIVAQQLIPTPDGQGRRACLEVFLNTPLAADLVRKGEVHELKELMKRSTEQGMQTFDQA
LYELYDSGEITYEDALSHADSPNDLRLMIKLGSESDADYLSNAADELSIQEDESNRSRRF

Nucleotide


Download         Length: 1143 bp        

>NTDB_id=1021432 AB1S68_RS02475 WP_011470109.1 566383..567525(+) (pilU) [Saccharophagus degradans strain HMH0024]
ATGGAATTAGATAGACTGCTGAGTTTAATGGTAGATAAACAGGCTTCGGATCTGTTTATTACGGCTGGGGTGGCGCCTTC
CATTAAGCTGCACGGTAAAATTGTTCCGGTAAGTACCACGCCGCTTTCGCCAGAAAAGTCGCGCGAGCTGGTGCTTAGCG
TAATGAATGAAAAACAGCGCAAAGAATTCTTAGAAGCCAAAGAGCTTAACTTTGCGGTAAGTGCGCGCGGTGTGGGTCGA
TTCCGTGCCAGCGCCTTTTACCAGCGCAACCTGGCGGGCATGGTGCTGCGTCGTATTGAAACCAATATTCCGCAAATTGA
TGAGTTGGGCCTACCCGAAATTATCAAAGAGCTGGCGATGATTAAGCGTGGCTTGGTGATGTTTGTGGGGGCAACCGGTA
CCGGTAAATCCACCTCGTTAGCCGCGATGATTGGCCACCGCAATCGCAACTCCAAGGGCCATATTATTTCGATAGAAGAC
CCGATTGAATTTATCCACCAGCATCAGGGGTGTATTGTTACCCAGCGCGAAGTGGGTATCGATACCGATTCTTTCGAGGT
AGCGCTAAAAAATACCCTGCGGCAGGCACCTGATGTTATTTTGATTGGTGAGGTTCGCTCGCGCGAAACTATGGACCACG
CAATCGCCTTCGCCGAAACGGGTCACCTGTGTCTATGTACGTTGCACGCCAACAACGCTAACCAAGCGTTGGATCGTATT
ATTCACTTCTTCCCCGCCGATCGTCACCGCCAGTTGTGGATGGATCTGTCGCTTAACCTAAAAGGTATTGTGGCGCAGCA
GCTTATTCCAACACCCGATGGACAAGGGCGCAGGGCGTGTTTGGAAGTGTTTTTGAATACACCTCTAGCTGCAGATTTAG
TGCGCAAGGGCGAAGTGCACGAGCTAAAAGAGTTGATGAAGCGCTCCACTGAGCAGGGCATGCAAACCTTCGATCAGGCG
CTGTACGAGCTTTACGATTCGGGCGAAATTACCTACGAAGATGCGCTATCCCACGCGGATTCGCCCAACGACCTGCGCTT
GATGATCAAGCTTGGTTCGGAAAGCGATGCCGATTACCTCTCCAACGCTGCGGATGAGTTAAGTATTCAAGAGGATGAAA
GCAACCGCTCGCGCCGTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(34-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q21EI5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

76.842

100

0.768
  pilU Acinetobacter baylyi ADP1

66.947

93.947

0.629
  pilU Vibrio cholerae strain A1552

55.556

97.105

0.539
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.188

90.789

0.392
  pilT Legionella pneumophila strain Lp02

40.896

88.158

0.361
  pilT Legionella pneumophila strain ERS1305867

40.896

88.158

0.361

Multiple sequence alignment