Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABZP20_RS13200 Genome accession   NZ_CP160433
Coordinates   2724455..2725498 (-) Length   347 a.a.
NCBI ID   WP_004631600.1    Uniprot ID   -
Organism   Ralstonia sp. CP     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2719455..2730498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABZP20_RS13170 (ABZP20_13170) - 2719933..2720502 (-) 570 WP_004631610.1 PilX N-terminal domain-containing pilus assembly protein -
  ABZP20_RS13175 (ABZP20_13175) - 2720505..2721542 (-) 1038 WP_004631609.1 PilW family protein -
  ABZP20_RS13180 (ABZP20_13180) pilV 2721539..2722135 (-) 597 WP_004631607.1 type IV pilus modification protein PilV -
  ABZP20_RS13185 (ABZP20_13185) - 2722132..2722605 (-) 474 WP_004631605.1 GspH/FimT family pseudopilin -
  ABZP20_RS13190 (ABZP20_13190) - 2722614..2723051 (-) 438 WP_004631602.1 type IV pilin protein -
  ABZP20_RS13195 (ABZP20_13195) pilU 2723249..2724388 (-) 1140 WP_004631601.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABZP20_RS13200 (ABZP20_13200) pilT 2724455..2725498 (-) 1044 WP_004631600.1 type IV pilus twitching motility protein PilT Machinery gene
  ABZP20_RS13205 (ABZP20_13205) - 2725621..2726337 (+) 717 WP_004631599.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABZP20_RS13210 (ABZP20_13210) proC 2726369..2727193 (+) 825 WP_024542538.1 pyrroline-5-carboxylate reductase -
  ABZP20_RS13215 (ABZP20_13215) ubiA 2727259..2728134 (-) 876 WP_004631597.1 4-hydroxybenzoate octaprenyltransferase -
  ABZP20_RS13220 (ABZP20_13220) katG 2728202..2730367 (-) 2166 WP_012436375.1 catalase/peroxidase HPI -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38194.82 Da        Isoelectric Point: 6.8307

>NTDB_id=1021098 ABZP20_RS13200 WP_004631600.1 2724455..2725498(-) (pilT) [Ralstonia sp. CP]
MDIAQLLAFSAKNKASDLHLSAGLPPMIRIHGDMRRINVPPLTHQDVHSMVYDIMSDGQRKVYEENLEVDFSFEIPGLSR
FRVNAFNQNRGASAVFRTIPSKVLSLDDLKAPAVFADLAMKPRGLVLVTGPTGSGKSTTLAAMVNHRNENDMGHILTVED
PIEFVHESKKSLINQRELGPHTHSFANALKSALREDPDVVLVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRV
VDVFPSDEKEMVRTMLSESLEAVISQTLLKTRDGSGRIAAHEIMIATPAIRHLIRENKISQMYSMMQTSSGMGMQTLDQC
LSELIKRSAINYADARAIAKNPDAFVS

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1021098 ABZP20_RS13200 WP_004631600.1 2724455..2725498(-) (pilT) [Ralstonia sp. CP]
ATGGACATCGCGCAGCTTCTGGCGTTTTCCGCCAAGAACAAGGCGTCGGACTTGCACCTGTCGGCCGGACTGCCGCCGAT
GATCCGGATCCACGGCGACATGCGCCGGATCAACGTGCCCCCGCTCACGCACCAAGATGTGCACTCCATGGTGTACGACA
TCATGAGCGACGGGCAGCGCAAGGTCTACGAAGAAAACCTGGAAGTCGACTTCTCGTTTGAGATCCCGGGGCTCTCGCGT
TTCCGGGTCAATGCGTTCAACCAGAATCGCGGCGCTTCGGCCGTGTTCCGGACGATTCCGTCGAAGGTGCTCTCGCTGGA
CGACCTGAAAGCGCCGGCCGTGTTTGCCGATCTGGCGATGAAGCCGCGCGGTCTGGTGCTCGTCACGGGCCCGACCGGCT
CGGGCAAGTCGACGACGCTTGCCGCGATGGTCAACCACCGCAACGAAAACGACATGGGCCACATCCTCACGGTGGAGGAC
CCGATCGAATTCGTGCACGAATCGAAGAAGAGCCTGATCAACCAGCGTGAACTCGGGCCCCATACCCATTCGTTCGCCAA
CGCGCTGAAGTCGGCGCTGCGTGAAGACCCGGATGTGGTGCTGGTCGGCGAATTGCGTGACCTTGAAACCATTCGCCTCG
CGCTTACCGCCGCAGAAACCGGCCACTTGGTGTTCGGCACGCTGCACACCAGCTCGGCAGCCAAGACCATCGACCGGGTC
GTCGACGTGTTTCCGTCGGACGAAAAAGAGATGGTCCGGACCATGCTTTCCGAATCGCTGGAGGCGGTGATCTCGCAGAC
GCTGCTCAAGACGCGCGATGGTTCGGGCCGGATCGCGGCGCACGAAATCATGATTGCCACGCCGGCCATTCGTCACTTGA
TCCGCGAGAACAAGATCTCGCAGATGTACTCGATGATGCAGACCAGCAGCGGCATGGGCATGCAGACGCTCGACCAGTGC
CTGTCGGAGCTGATCAAGCGCTCGGCGATCAACTACGCAGACGCGCGCGCCATTGCCAAGAACCCAGACGCGTTCGTGAG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

70.809

99.712

0.706
  pilT Acinetobacter baumannii D1279779

70.809

99.712

0.706
  pilT Acinetobacter baumannii strain A118

70.809

99.712

0.706
  pilT Pseudomonas aeruginosa PAK

70.145

99.424

0.697
  pilT Pseudomonas stutzeri DSM 10701

69.855

99.424

0.695
  pilT Acinetobacter baylyi ADP1

68.986

99.424

0.686
  pilT Neisseria gonorrhoeae MS11

68.986

99.424

0.686
  pilT Neisseria meningitidis 8013

68.986

99.424

0.686
  pilT Legionella pneumophila strain Lp02

66.957

99.424

0.666
  pilT Legionella pneumophila strain ERS1305867

66.957

99.424

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.172

97.118

0.643
  pilT Vibrio cholerae strain A1552

66.172

97.118

0.643
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.145

99.424

0.499
  pilU Acinetobacter baylyi ADP1

42.733

99.135

0.424
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403
  pilU Vibrio cholerae strain A1552

40.896

96.542

0.395

Multiple sequence alignment