Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABZP20_RS13195 Genome accession   NZ_CP160433
Coordinates   2723249..2724388 (-) Length   379 a.a.
NCBI ID   WP_004631601.1    Uniprot ID   R0CJW1
Organism   Ralstonia sp. CP     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2718249..2729388
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABZP20_RS13170 (ABZP20_13170) - 2719933..2720502 (-) 570 WP_004631610.1 PilX N-terminal domain-containing pilus assembly protein -
  ABZP20_RS13175 (ABZP20_13175) - 2720505..2721542 (-) 1038 WP_004631609.1 PilW family protein -
  ABZP20_RS13180 (ABZP20_13180) pilV 2721539..2722135 (-) 597 WP_004631607.1 type IV pilus modification protein PilV -
  ABZP20_RS13185 (ABZP20_13185) - 2722132..2722605 (-) 474 WP_004631605.1 GspH/FimT family pseudopilin -
  ABZP20_RS13190 (ABZP20_13190) - 2722614..2723051 (-) 438 WP_004631602.1 type IV pilin protein -
  ABZP20_RS13195 (ABZP20_13195) pilU 2723249..2724388 (-) 1140 WP_004631601.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABZP20_RS13200 (ABZP20_13200) pilT 2724455..2725498 (-) 1044 WP_004631600.1 type IV pilus twitching motility protein PilT Machinery gene
  ABZP20_RS13205 (ABZP20_13205) - 2725621..2726337 (+) 717 WP_004631599.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABZP20_RS13210 (ABZP20_13210) proC 2726369..2727193 (+) 825 WP_024542538.1 pyrroline-5-carboxylate reductase -
  ABZP20_RS13215 (ABZP20_13215) ubiA 2727259..2728134 (-) 876 WP_004631597.1 4-hydroxybenzoate octaprenyltransferase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42224.53 Da        Isoelectric Point: 6.6638

>NTDB_id=1021097 ABZP20_RS13195 WP_004631601.1 2723249..2724388(-) (pilU) [Ralstonia sp. CP]
MLDREAATKYIHELLQLMVNSRGSDLFITAEFPPAIKVDGKVTPISQQPLNAQQAVGLVKAIMNEKQLREYEDSSECNFA
ITAPGAGRFRVSAFMQQGRAGMVLRTINTKIPTLGELDLPPILNEIVMSKRGLVIVVGATGSGKSTSLAAMVGYRNANSY
GHIITIEDPVEYVHAHQNCVVTQREVGVDTESWHVALKNTLRQAPDVILIGEIRDRDTMEYAIQYAETGHLCLATLHANS
SNQAIDRIINFFPEEKRQQLLIDLSLNLRAMIAQRLLPRQGKKGRVPAVEIMLATPLVQDLIFKGEVHELKEVMKKSREQ
GMISFDQALFDLYEENKITYEDALRNADSLNDLRLQIKLHSKRGGQTDLSAGTEHLNVV

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1021097 ABZP20_RS13195 WP_004631601.1 2723249..2724388(-) (pilU) [Ralstonia sp. CP]
ATGCTGGACCGCGAAGCCGCCACCAAATACATCCACGAACTCTTGCAGTTGATGGTGAACAGCCGTGGCTCGGACTTGTT
CATCACGGCAGAATTTCCGCCCGCGATCAAGGTGGACGGCAAGGTCACGCCGATCTCGCAGCAGCCGCTGAATGCCCAGC
AGGCCGTCGGTCTGGTCAAGGCGATCATGAACGAGAAGCAGCTGCGCGAGTACGAAGACAGCTCGGAATGCAACTTCGCC
ATCACGGCGCCGGGCGCCGGGCGATTCCGCGTGTCGGCGTTCATGCAGCAAGGTCGCGCCGGCATGGTGCTGCGGACCAT
CAACACGAAGATCCCGACGCTGGGCGAGTTGGACCTGCCGCCGATCCTCAATGAGATCGTGATGAGCAAGCGCGGCCTCG
TGATCGTCGTGGGCGCCACGGGCTCGGGTAAGTCGACCTCGCTGGCGGCGATGGTGGGCTATCGCAATGCGAATTCGTAC
GGCCACATCATCACCATTGAAGATCCGGTGGAATACGTGCACGCGCACCAGAACTGCGTGGTGACGCAGCGCGAGGTGGG
TGTGGATACCGAGTCGTGGCACGTGGCGCTGAAGAACACGCTGCGGCAGGCGCCGGACGTGATCCTGATCGGCGAAATCC
GGGATCGCGACACGATGGAATACGCCATCCAGTACGCGGAAACCGGCCACTTGTGCCTTGCCACGCTGCACGCCAACAGC
TCGAACCAGGCGATCGACCGGATCATCAACTTCTTCCCGGAAGAGAAGCGTCAGCAGCTGCTGATCGACCTGTCGCTGAA
CCTGCGCGCGATGATTGCGCAACGCCTGCTGCCGCGTCAGGGCAAGAAGGGGCGCGTACCGGCCGTCGAGATCATGCTGG
CGACCCCGCTGGTGCAAGACCTCATCTTCAAGGGCGAGGTGCACGAGCTGAAGGAGGTCATGAAGAAATCCCGCGAGCAA
GGGATGATCTCGTTCGACCAGGCGCTGTTCGATTTGTACGAAGAAAACAAGATCACGTACGAAGACGCGCTGCGTAATGC
CGACTCTCTCAACGATCTGCGGCTGCAGATCAAACTGCACAGCAAGCGGGGCGGGCAGACGGACCTGTCGGCCGGCACCG
AGCATTTGAATGTCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(37-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R0CJW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.326

95.515

0.586
  pilU Acinetobacter baylyi ADP1

55.429

92.348

0.512
  pilU Vibrio cholerae strain A1552

53.39

93.404

0.499
  pilT Acinetobacter baylyi ADP1

43.844

87.863

0.385
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.323

88.918

0.385
  pilT Acinetobacter baumannii strain A118

43.544

87.863

0.383
  pilT Acinetobacter baumannii D1279779

43.544

87.863

0.383
  pilT Acinetobacter nosocomialis M2

43.544

87.863

0.383
  pilT Vibrio cholerae strain A1552

45.741

83.641

0.383
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

45.741

83.641

0.383
  pilT Legionella pneumophila strain ERS1305867

43.243

87.863

0.38
  pilT Legionella pneumophila strain Lp02

43.243

87.863

0.38
  pilT Pseudomonas aeruginosa PAK

42.433

88.918

0.377
  pilT Pseudomonas stutzeri DSM 10701

41.84

88.918

0.372

Multiple sequence alignment