Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AB0A83_RS20090 Genome accession   NZ_CP160405
Coordinates   4466002..4467039 (+) Length   345 a.a.
NCBI ID   WP_150051580.1    Uniprot ID   -
Organism   Methylomonas sp. HYX-M1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4461002..4472039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0A83_RS20060 (AB0A83_20060) - 4461103..4461258 (-) 156 WP_367155036.1 hypothetical protein -
  AB0A83_RS20065 (AB0A83_20065) - 4461626..4462192 (-) 567 WP_150051572.1 Smr/MutS family protein -
  AB0A83_RS20070 (AB0A83_20070) surE 4462256..4462996 (+) 741 WP_150052114.1 5'/3'-nucleotidase SurE -
  AB0A83_RS20075 (AB0A83_20075) - 4463035..4463667 (+) 633 WP_197737448.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase -
  AB0A83_RS20080 (AB0A83_20080) - 4464137..4465369 (-) 1233 WP_150051576.1 NADP-dependent isocitrate dehydrogenase -
  AB0A83_RS20085 (AB0A83_20085) - 4465494..4465838 (+) 345 WP_367155037.1 TraR/DksA family transcriptional regulator -
  AB0A83_RS20090 (AB0A83_20090) pilT 4466002..4467039 (+) 1038 WP_150051580.1 type IV pilus twitching motility protein PilT Machinery gene
  AB0A83_RS20095 (AB0A83_20095) pilU 4467063..4468199 (+) 1137 WP_150051582.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB0A83_RS20100 (AB0A83_20100) mltB 4468375..4469361 (+) 987 WP_367155038.1 lytic murein transglycosylase B -
  AB0A83_RS20105 (AB0A83_20105) dapD 4469404..4470222 (-) 819 WP_150051586.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  AB0A83_RS20110 (AB0A83_20110) dapC 4470323..4471522 (-) 1200 WP_150051588.1 succinyldiaminopimelate transaminase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38241.03 Da        Isoelectric Point: 6.8401

>NTDB_id=1021014 AB0A83_RS20090 WP_150051580.1 4466002..4467039(+) (pilT) [Methylomonas sp. HYX-M1]
MDIAELLTFSVKNKASDLHLSAGLPPMIRVDGDIRRINIPALEHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPGVAR
FRVNAFNQDRGAGAVFRTIPSKVLTLEELGAPKFFEEVCTKPRGLILVTGPTGSGKSTTLAAMINHINSNDYSHILTVED
PIEFVHESQKSLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRSMLSESLQAVISQTLLKKVGGGRIAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGRKDGMQTLDQNL
KELVDRGLITGKAAMTKAVNKDIFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1021014 AB0A83_RS20090 WP_150051580.1 4466002..4467039(+) (pilT) [Methylomonas sp. HYX-M1]
ATGGATATCGCCGAACTGTTGACCTTTTCCGTAAAAAACAAAGCTTCCGACTTGCACTTGTCCGCCGGCTTGCCGCCGAT
GATCAGGGTCGACGGCGATATTCGCCGCATCAACATTCCGGCCTTGGAGCATAAGGAAGTGCACGCGCTGATTTACGACA
TCATGAACGACAAGCAGCGGCGCGACTACGAAGAGTTTTTGGAAACCGACTTTTCCTTCGCCTTGCCCGGCGTGGCCCGT
TTCAGGGTCAATGCCTTCAACCAAGACCGCGGCGCCGGGGCGGTATTCAGGACTATTCCATCGAAAGTGCTGACCTTGGA
GGAACTCGGCGCGCCGAAATTTTTCGAAGAAGTCTGCACCAAGCCGCGCGGGCTGATTTTGGTTACCGGGCCCACCGGCT
CCGGCAAGTCCACCACGCTGGCGGCGATGATCAATCACATCAATTCCAACGATTACTCGCACATCCTGACCGTCGAAGAC
CCTATAGAATTCGTGCACGAAAGCCAGAAATCGCTGATCAACCAGCGGGAAGTGCATAGGGACACGCTAGGCTTCAACGA
AGCCTTGCGCTCGGCCTTGCGGGAAGACCCGGACATCATCCTGGTCGGCGAGATGCGCGACCTGGAAACCATACGCCTGG
CGTTGACCGCCGCGGAAACCGGCCACCTGGTATTCGGCACCCTGCATACCACCTCGGCGGCAAAAACCATAGACCGCATC
ATCGACGTCTTTCCGGCGGCGGAAAAAGACATGATACGCTCCATGCTGTCCGAGTCGCTGCAAGCCGTGATTTCTCAAAC
CTTGTTGAAAAAGGTGGGCGGCGGCCGAATTGCCGCGCACGAAATCATGGTCGGCACCCCGGCTATCCGCAACCTGATCC
GCGAGGCCAAGGTCGCGCAAATGTATTCCGCCATTCAAACCGGGCGCAAGGACGGCATGCAAACCCTGGATCAAAACCTG
AAGGAACTGGTCGATCGCGGCCTGATCACCGGAAAAGCGGCGATGACCAAGGCGGTCAACAAAGACATTTTCCGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

80.814

99.71

0.806
  pilT Acinetobacter baumannii strain A118

80.814

99.71

0.806
  pilT Acinetobacter nosocomialis M2

80.814

99.71

0.806
  pilT Pseudomonas aeruginosa PAK

81.361

97.971

0.797
  pilT Acinetobacter baylyi ADP1

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Legionella pneumophila strain Lp02

73.547

99.71

0.733
  pilT Legionella pneumophila strain ERS1305867

73.547

99.71

0.733
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.733

97.681

0.681
  pilT Vibrio cholerae strain A1552

69.733

97.681

0.681
  pilT Neisseria gonorrhoeae MS11

67.055

99.42

0.667
  pilT Neisseria meningitidis 8013

67.055

99.42

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Acinetobacter baylyi ADP1

40.588

98.551

0.4
  pilU Pseudomonas stutzeri DSM 10701

39.595

100

0.397
  pilU Vibrio cholerae strain A1552

39.595

100

0.397

Multiple sequence alignment