Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB0A83_RS20095 Genome accession   NZ_CP160405
Coordinates   4467063..4468199 (+) Length   378 a.a.
NCBI ID   WP_150051582.1    Uniprot ID   -
Organism   Methylomonas sp. HYX-M1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4462063..4473199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0A83_RS20070 (AB0A83_20070) surE 4462256..4462996 (+) 741 WP_150052114.1 5'/3'-nucleotidase SurE -
  AB0A83_RS20075 (AB0A83_20075) - 4463035..4463667 (+) 633 WP_197737448.1 protein-L-isoaspartate(D-aspartate) O-methyltransferase -
  AB0A83_RS20080 (AB0A83_20080) - 4464137..4465369 (-) 1233 WP_150051576.1 NADP-dependent isocitrate dehydrogenase -
  AB0A83_RS20085 (AB0A83_20085) - 4465494..4465838 (+) 345 WP_367155037.1 TraR/DksA family transcriptional regulator -
  AB0A83_RS20090 (AB0A83_20090) pilT 4466002..4467039 (+) 1038 WP_150051580.1 type IV pilus twitching motility protein PilT Machinery gene
  AB0A83_RS20095 (AB0A83_20095) pilU 4467063..4468199 (+) 1137 WP_150051582.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB0A83_RS20100 (AB0A83_20100) mltB 4468375..4469361 (+) 987 WP_367155038.1 lytic murein transglycosylase B -
  AB0A83_RS20105 (AB0A83_20105) dapD 4469404..4470222 (-) 819 WP_150051586.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  AB0A83_RS20110 (AB0A83_20110) dapC 4470323..4471522 (-) 1200 WP_150051588.1 succinyldiaminopimelate transaminase -
  AB0A83_RS20115 (AB0A83_20115) - 4471529..4472314 (-) 786 WP_150051590.1 M48 family metallopeptidase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 42418.88 Da        Isoelectric Point: 6.6475

>NTDB_id=1021015 AB0A83_RS20095 WP_150051582.1 4467063..4468199(+) (pilU) [Methylomonas sp. HYX-M1]
MDFKALLALMVEKKASDLFITAGKPPCMKIDGKVVEISKNILTAEQALKVVHSIMDQRQKDEFEHTKECQFALGVHGLGR
FRVSAFTQRDAAGMVLRRIETHIPDTEELHLPPVLKELIMHKRGLVMFVGATGTGKSTSLAALIKHRNANSSGHIITIED
PIEFVHPHNGCIITQREVGLDTESYEVALKNTLRQAPDVILIGEVRTKETMQHAITFAETGHLCLCTLHANNANQALDRI
LHFFPEEMHNQIFMDLSLNLRGIVAQQLVKRADGNGRYPAIEILLNTPLVSDLIRKGEVHKLKELMKNSREHGMQTFDQA
LFDLYAAGKISYEDALLAADSRNEVRLMIKLSTETGGFDDENMRLSETDEESGRLYKF

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1021015 AB0A83_RS20095 WP_150051582.1 4467063..4468199(+) (pilU) [Methylomonas sp. HYX-M1]
ATGGACTTCAAAGCGTTATTGGCCTTGATGGTGGAAAAAAAAGCATCCGACTTGTTCATCACCGCCGGCAAACCGCCGTG
CATGAAAATCGACGGCAAAGTGGTGGAGATTTCAAAAAACATTCTTACCGCCGAACAGGCGCTGAAAGTGGTGCACAGCA
TCATGGACCAGCGGCAGAAAGACGAATTCGAACACACCAAAGAATGCCAGTTCGCCCTGGGGGTACACGGCCTGGGCCGG
TTCCGGGTTAGCGCCTTTACCCAACGCGACGCGGCCGGCATGGTGTTGCGCCGCATCGAAACCCATATCCCCGATACCGA
AGAACTGCACTTGCCGCCGGTTTTGAAAGAACTGATCATGCATAAACGCGGTTTGGTGATGTTCGTCGGCGCCACCGGCA
CCGGTAAATCCACATCGCTGGCAGCCTTGATCAAGCACCGCAACGCGAACAGCAGCGGCCACATCATCACCATCGAAGAT
CCGATAGAATTCGTGCATCCGCATAACGGCTGCATCATCACGCAACGCGAGGTCGGCCTGGATACCGAATCCTACGAGGT
GGCGTTGAAAAACACCTTGCGGCAAGCGCCGGACGTGATTCTGATCGGCGAGGTCAGAACCAAGGAAACCATGCAACACG
CCATCACCTTCGCCGAAACCGGCCATTTGTGCTTGTGCACCTTGCACGCCAACAACGCCAATCAAGCTTTGGACCGCATC
CTGCATTTCTTTCCGGAAGAAATGCACAACCAAATTTTTATGGACTTATCCTTGAATCTACGCGGTATCGTCGCCCAGCA
ACTGGTAAAACGGGCCGACGGCAACGGCCGCTATCCGGCCATCGAAATATTGCTGAATACGCCGCTGGTTTCCGATTTGA
TTCGCAAGGGCGAAGTGCATAAACTGAAAGAATTGATGAAAAACTCGCGCGAACACGGCATGCAGACCTTTGACCAAGCC
TTGTTCGATTTGTACGCCGCCGGCAAAATCAGCTACGAAGACGCTCTGCTTGCAGCGGATTCCAGAAACGAAGTGCGCTT
GATGATCAAGCTGAGCACGGAAACCGGTGGTTTCGACGACGAAAACATGCGTTTAAGCGAAACCGATGAAGAAAGCGGTC
GATTGTATAAATTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

64.829

100

0.653
  pilU Acinetobacter baylyi ADP1

61.892

97.884

0.606
  pilU Vibrio cholerae strain A1552

57.429

92.593

0.532
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.575

94.709

0.413
  pilT Legionella pneumophila strain Lp02

42.773

89.683

0.384
  pilT Legionella pneumophila strain ERS1305867

42.773

89.683

0.384
  pilT Pseudomonas aeruginosa PAK

41.791

88.624

0.37
  pilT Vibrio cholerae strain A1552

41.916

88.36

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.916

88.36

0.37
  pilT Acinetobacter baumannii D1279779

41.003

89.683

0.368
  pilT Acinetobacter baumannii strain A118

41.003

89.683

0.368
  pilT Acinetobacter nosocomialis M2

40.708

89.683

0.365
  pilT Acinetobacter baylyi ADP1

40.469

90.212

0.365

Multiple sequence alignment