Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABUE11_RS01900 Genome accession   NZ_CP160388
Coordinates   391552..392595 (-) Length   347 a.a.
NCBI ID   WP_367067333.1    Uniprot ID   -
Organism   Oryzisolibacter sp. LB2S     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 386552..397595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABUE11_RS01870 - 386987..387388 (+) 402 WP_367067325.1 YraN family protein -
  ABUE11_RS01875 - 387448..388044 (+) 597 WP_367067326.1 SIS domain-containing protein -
  ABUE11_RS01880 - 388041..388697 (+) 657 WP_367067327.1 BON domain-containing protein -
  ABUE11_RS01885 - 388710..389636 (-) 927 WP_367067329.1 NAD(P)-dependent oxidoreductase -
  ABUE11_RS01890 pilU 389705..390841 (-) 1137 WP_367067330.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABUE11_RS01895 - 390872..391495 (-) 624 WP_367067332.1 cyclic nucleotide-binding domain-containing protein -
  ABUE11_RS01900 pilT 391552..392595 (-) 1044 WP_367067333.1 type IV pilus twitching motility protein PilT Machinery gene
  ABUE11_RS01905 - 392634..393359 (+) 726 WP_367067334.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABUE11_RS01910 - 393405..394544 (+) 1140 WP_367067335.1 aminotransferase class V-fold PLP-dependent enzyme -
  ABUE11_RS01915 - 394590..395942 (+) 1353 WP_367067337.1 DUF445 domain-containing protein -
  ABUE11_RS01920 - 396022..396717 (-) 696 WP_367067339.1 MarC family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38130.91 Da        Isoelectric Point: 6.8353

>NTDB_id=1020752 ABUE11_RS01900 WP_367067333.1 391552..392595(-) (pilT) [Oryzisolibacter sp. LB2S]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVDPLDHKTVHAMVYDIMSDAQRKAYEEFLEVDFSFEIDGLAR
FRVNAFNQNRGAAAVFRTIPSKILTLEQLNAPKIFADLALKPRGLVLVTGPTGSGKSTTLAGMINHLNETEYGHILTIED
PIEFVHESKKCLVNQREVGPMTLSFSAALRSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMVRAMLSESLQAVISQTLCKLKDGSGRVAAHEIMLGTSAIRNLIREAKVAQMYSTIQTSNSVGMQTLDQN
LADLVRRNIISPAEARSKAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1020752 ABUE11_RS01900 WP_367067333.1 391552..392595(-) (pilT) [Oryzisolibacter sp. LB2S]
GTGGACATCACCCAGTTGCTCGCCTTCAGCGTCAAGAACAAGGCCTCGGACCTGCATCTGTCTGCGGGCCTGCCTCCCAT
GATCCGGGTACATGGCGACGTGCGGCGCATCAATGTCGATCCGCTCGACCACAAGACCGTGCATGCCATGGTGTACGACA
TCATGAGCGACGCACAACGCAAGGCCTATGAGGAGTTTCTGGAGGTCGACTTCTCCTTCGAGATCGATGGTCTGGCGCGC
TTTCGCGTCAACGCCTTCAACCAGAACCGCGGCGCGGCCGCCGTGTTCCGCACCATTCCGAGCAAGATCCTGACGCTCGA
GCAGCTCAACGCCCCCAAGATTTTTGCCGATCTGGCGCTCAAGCCCCGTGGCCTGGTGCTGGTGACCGGCCCCACGGGTT
CGGGCAAGTCGACGACGCTGGCGGGCATGATCAACCACCTCAACGAAACCGAGTATGGCCATATCCTGACCATCGAGGAT
CCCATCGAGTTCGTGCACGAATCCAAGAAATGCCTGGTCAACCAGCGCGAGGTCGGCCCCATGACGCTGTCGTTTTCCGC
CGCGCTGCGCTCGGCTCTGCGCGAGGACCCGGATGCCATCCTCGTGGGCGAAATGCGCGACCTCGAGACCATCCGCCTGG
CCATGACCGCGGCCGAGACCGGCCACCTGGTGTTCGGCACGCTGCACACCAGCAGCGCCGCCAAGACCATAGACCGCATC
ATCGACGTCTTCCCCGCCGAGGAAAAGGAGATGGTGCGCGCCATGCTGTCGGAGTCGCTGCAGGCCGTGATCTCGCAGAC
CCTGTGCAAGCTCAAGGACGGCTCGGGCCGCGTCGCCGCGCACGAGATCATGCTCGGCACCAGTGCCATCCGCAACCTGA
TTCGCGAAGCCAAGGTGGCGCAGATGTATTCCACGATTCAGACCAGCAACAGCGTCGGCATGCAGACGCTGGACCAGAAC
CTGGCCGACCTGGTGCGACGCAACATCATCAGTCCCGCGGAAGCACGCAGCAAGGCCAAGATCCCGGACAACTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.232

99.424

0.758
  pilT Acinetobacter nosocomialis M2

75.072

99.424

0.746
  pilT Acinetobacter baumannii D1279779

75.072

99.424

0.746
  pilT Acinetobacter baumannii strain A118

75.072

99.424

0.746
  pilT Acinetobacter baylyi ADP1

73.913

99.424

0.735
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Neisseria meningitidis 8013

68.235

97.983

0.669
  pilT Neisseria gonorrhoeae MS11

67.941

97.983

0.666
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.862

98.271

0.657
  pilT Vibrio cholerae strain A1552

66.862

98.271

0.657
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.594

99.424

0.513
  pilU Vibrio cholerae strain A1552

43.582

96.542

0.421
  pilU Acinetobacter baylyi ADP1

41.04

99.712

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403

Multiple sequence alignment