Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABUE11_RS01890 Genome accession   NZ_CP160388
Coordinates   389705..390841 (-) Length   378 a.a.
NCBI ID   WP_367067330.1    Uniprot ID   -
Organism   Oryzisolibacter sp. LB2S     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 384705..395841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABUE11_RS01855 - 385124..385816 (+) 693 WP_367067323.1 response regulator -
  ABUE11_RS01865 rsmI 386064..386987 (-) 924 WP_367067324.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  ABUE11_RS01870 - 386987..387388 (+) 402 WP_367067325.1 YraN family protein -
  ABUE11_RS01875 - 387448..388044 (+) 597 WP_367067326.1 SIS domain-containing protein -
  ABUE11_RS01880 - 388041..388697 (+) 657 WP_367067327.1 BON domain-containing protein -
  ABUE11_RS01885 - 388710..389636 (-) 927 WP_367067329.1 NAD(P)-dependent oxidoreductase -
  ABUE11_RS01890 pilU 389705..390841 (-) 1137 WP_367067330.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABUE11_RS01895 - 390872..391495 (-) 624 WP_367067332.1 cyclic nucleotide-binding domain-containing protein -
  ABUE11_RS01900 pilT 391552..392595 (-) 1044 WP_367067333.1 type IV pilus twitching motility protein PilT Machinery gene
  ABUE11_RS01905 - 392634..393359 (+) 726 WP_367067334.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABUE11_RS01910 - 393405..394544 (+) 1140 WP_367067335.1 aminotransferase class V-fold PLP-dependent enzyme -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41879.37 Da        Isoelectric Point: 6.7688

>NTDB_id=1020751 ABUE11_RS01890 WP_367067330.1 389705..390841(-) (pilU) [Oryzisolibacter sp. LB2S]
MERDQASKFINDLLKLMISRRGSDLFITAEFPPAIKVDGKVTKVSPQPLTPAHTLTLARSIMSDKQIADFERTKECNFAI
SPAGIGRFRVNAFIQQGHVGMVLRTIPLTLPTIDGLGMPEVLKDVSMTKRGLCILVGATGSGKSTTLAAMVDWRNENSFG
HIITIEDPIEFVHPHKNCVVTQREVGLDTDNWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCLATLHANSA
NQALDRIVNFFPEERRAQLLMDLSLNLRAMVSQRLIPKQDGKGRAAAVEIMLNTPLIADLIFKGEVAEIKEIMKKSRNLG
MQTFDQSLFDLFEAGMVSYEDALRNADSVNDLRLQIKLSSQRAKTTDLASGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1020751 ABUE11_RS01890 WP_367067330.1 389705..390841(-) (pilU) [Oryzisolibacter sp. LB2S]
ATGGAACGCGATCAGGCAAGCAAATTCATCAACGACCTGCTCAAGCTGATGATCAGCCGCAGGGGCAGCGACCTTTTCAT
CACCGCGGAGTTTCCGCCCGCGATCAAGGTGGACGGCAAGGTCACCAAGGTCTCGCCACAGCCCCTGACGCCTGCCCATA
CGCTCACGCTCGCGCGCTCGATCATGAGCGACAAGCAGATCGCGGACTTCGAGCGCACCAAGGAATGCAATTTCGCCATC
TCGCCGGCCGGCATAGGGCGGTTTCGCGTGAACGCCTTCATTCAACAGGGCCATGTCGGCATGGTGCTGCGCACGATTCC
CCTCACGCTGCCGACCATCGACGGTCTGGGCATGCCCGAGGTGCTCAAGGACGTCTCCATGACCAAGCGTGGCCTGTGCA
TTCTGGTCGGCGCCACGGGCTCGGGCAAATCCACCACGCTCGCAGCCATGGTCGACTGGCGCAACGAAAACTCCTTCGGC
CACATCATCACCATCGAGGACCCTATCGAATTCGTGCACCCGCACAAGAACTGCGTGGTCACCCAGCGCGAGGTGGGCCT
GGACACCGACAACTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAAGCGCCCGACGTGATCCTGATGGGCGAGATTCGCG
ACCGCGAGACCATGGAGCATGCCGTGGCCTTTGCCGAGACCGGCCACCTGTGCCTGGCGACACTGCACGCCAACAGCGCC
AACCAGGCGCTCGATCGCATCGTCAACTTCTTCCCCGAGGAGCGGCGCGCCCAGCTGCTCATGGACCTGTCGCTCAACCT
GCGCGCCATGGTCTCGCAGCGCCTGATCCCCAAGCAGGACGGCAAGGGCCGGGCGGCCGCCGTGGAAATCATGCTCAACA
CCCCCCTGATCGCCGACCTGATCTTCAAGGGCGAGGTCGCCGAGATCAAGGAGATCATGAAGAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAATCGCTGTTCGACCTGTTCGAGGCCGGCATGGTCAGCTACGAGGACGCCCTGCGCAACGCCGA
CTCGGTCAACGACCTGCGCCTGCAGATCAAACTCTCCAGCCAGCGTGCCAAGACCACCGACCTGGCCTCCGGCACCGAGC
ATTTCGCCATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

57.263

94.709

0.542
  pilU Vibrio cholerae strain A1552

52.066

96.032

0.5
  pilT Pseudomonas aeruginosa PAK

47.181

89.153

0.421
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.807

89.153

0.399
  pilT Pseudomonas stutzeri DSM 10701

44.807

89.153

0.399
  pilT Acinetobacter baumannii D1279779

44.144

88.095

0.389
  pilT Acinetobacter nosocomialis M2

44.144

88.095

0.389
  pilT Acinetobacter baumannii strain A118

44.144

88.095

0.389
  pilT Acinetobacter baylyi ADP1

43.243

88.095

0.381
  pilT Legionella pneumophila strain Lp02

42.943

88.095

0.378
  pilT Legionella pneumophila strain ERS1305867

42.943

88.095

0.378
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.47

87.831

0.373
  pilT Vibrio cholerae strain A1552

42.47

87.831

0.373

Multiple sequence alignment