Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABXT62_RS16335 Genome accession   NZ_CP159826
Coordinates   3619467..3620501 (-) Length   344 a.a.
NCBI ID   WP_415356376.1    Uniprot ID   -
Organism   Halioglobus sp. Uisw_031     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3614467..3625501
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXT62_RS16310 (ABXT62_16320) gshB 3615343..3616299 (+) 957 WP_415356371.1 glutathione synthase -
  ABXT62_RS16315 (ABXT62_16325) - 3616307..3617173 (+) 867 WP_415356372.1 energy transducer TonB -
  ABXT62_RS16320 (ABXT62_16330) - 3617228..3617818 (+) 591 WP_415356373.1 YqgE/AlgH family protein -
  ABXT62_RS16325 (ABXT62_16335) ruvX 3617845..3618276 (+) 432 WP_415356374.1 Holliday junction resolvase RuvX -
  ABXT62_RS16330 (ABXT62_16340) pilU 3618289..3619428 (-) 1140 WP_415356375.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABXT62_RS16335 (ABXT62_16345) pilT 3619467..3620501 (-) 1035 WP_415356376.1 type IV pilus twitching motility protein PilT Machinery gene
  ABXT62_RS16340 (ABXT62_16350) - 3620627..3621319 (+) 693 WP_415356377.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABXT62_RS16345 (ABXT62_16355) proC 3621372..3622208 (+) 837 WP_415356378.1 pyrroline-5-carboxylate reductase -
  ABXT62_RS16350 (ABXT62_16360) - 3622225..3622812 (+) 588 WP_415356379.1 YggT family protein -
  ABXT62_RS16355 (ABXT62_16365) metX 3622823..3623971 (+) 1149 WP_415358235.1 homoserine O-succinyltransferase MetX -
  ABXT62_RS16360 (ABXT62_16370) metW 3623971..3624561 (+) 591 WP_415356380.1 methionine biosynthesis protein MetW -
  ABXT62_RS16365 (ABXT62_16375) - 3624563..3625003 (+) 441 WP_415356381.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38359.23 Da        Isoelectric Point: 6.6661

>NTDB_id=1016079 ABXT62_RS16335 WP_415356376.1 3619467..3620501(-) (pilT) [Halioglobus sp. Uisw_031]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDIRRVNLPPMDHKQVHELIYDIMNDKQRKDYEEFLETDFSFEVPGIAR
FRVNAFNQNRGAAGVFRTIPSKVLSMEDLGMGQVFRDISMMPRGLVLVTGPTGSGKSTTMAAMVDFINDNKYEHILTIED
PIEFVHESKKCLVNQREVHRHTLGFAEALRSALREDPDIVLVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAAEKSMVRSMLSESLQAVVSQTLLKKATGGRIAAHEIMRGTSAIRNLIREDKVAQMYSAIQTGSALGMQTMDQCL
EQMVGKRLITREVAREKARMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1016079 ABXT62_RS16335 WP_415356376.1 3619467..3620501(-) (pilT) [Halioglobus sp. Uisw_031]
ATGGATATAACAGAATTACTGGCCTTTAGTGCCAAGCAGGGCGCGTCTGATCTACACCTTTCAGCGGGCCTGCCGCCAAT
GATCCGTGTTGACGGCGATATTCGCCGTGTCAACCTGCCACCGATGGACCACAAACAGGTTCACGAACTCATTTACGATA
TCATGAACGACAAGCAGCGTAAGGACTATGAAGAGTTTCTCGAGACAGACTTTTCCTTTGAAGTGCCTGGCATCGCGCGC
TTTAGAGTCAATGCGTTCAACCAAAATAGAGGCGCCGCAGGTGTGTTTCGCACTATCCCCTCCAAGGTTTTGTCCATGGA
AGATCTCGGAATGGGGCAGGTTTTTCGGGATATTTCGATGATGCCTCGCGGGCTGGTGCTGGTGACCGGGCCCACCGGTT
CGGGCAAGTCCACTACTATGGCGGCCATGGTGGATTTTATCAACGACAATAAATACGAACATATTCTCACTATCGAAGAC
CCCATTGAATTTGTACATGAAAGTAAGAAGTGCCTTGTAAATCAACGCGAAGTGCACCGCCATACCCTGGGCTTTGCTGA
AGCATTGCGGTCAGCGCTGCGGGAAGACCCCGATATTGTGCTGGTGGGAGAGATGCGTGATCTGGAAACCATACGCCTAG
CGTTGACGGCAGCGGAAACCGGGCACCTAGTGTTTGGCACGCTGCATACGACCTCAGCAGCTAAGACGATTGACAGAGTG
ATTGATGTTTTTCCTGCTGCGGAAAAGTCTATGGTGCGCTCCATGTTGTCGGAATCACTGCAAGCGGTGGTATCACAAAC
GCTACTAAAAAAAGCCACCGGTGGACGGATAGCGGCCCATGAAATTATGCGTGGCACCTCCGCAATACGGAATTTGATTC
GTGAAGACAAGGTGGCGCAGATGTATTCCGCTATCCAGACGGGCAGCGCATTAGGCATGCAAACGATGGACCAGTGTTTA
GAGCAAATGGTAGGGAAGCGCCTCATCACCCGCGAAGTGGCGCGCGAAAAAGCCAGGATGCCGGAGAACTTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

85.465

100

0.855
  pilT Pseudomonas aeruginosa PAK

85.174

100

0.852
  pilT Acinetobacter baumannii D1279779

79.07

100

0.791
  pilT Acinetobacter baumannii strain A118

79.07

100

0.791
  pilT Acinetobacter nosocomialis M2

79.07

100

0.791
  pilT Acinetobacter baylyi ADP1

77.907

100

0.779
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

65.797

100

0.66
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.706

98.837

0.64
  pilT Vibrio cholerae strain A1552

64.706

98.837

0.64
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Acinetobacter baylyi ADP1

37.861

100

0.381
  pilU Vibrio cholerae strain A1552

38.154

94.477

0.36

Multiple sequence alignment