Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABXT62_RS16330 Genome accession   NZ_CP159826
Coordinates   3618289..3619428 (-) Length   379 a.a.
NCBI ID   WP_415356375.1    Uniprot ID   -
Organism   Halioglobus sp. Uisw_031     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3613289..3624428
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABXT62_RS16305 (ABXT62_16315) - 3613945..3615195 (-) 1251 WP_415356370.1 alpha/beta hydrolase fold domain-containing protein -
  ABXT62_RS16310 (ABXT62_16320) gshB 3615343..3616299 (+) 957 WP_415356371.1 glutathione synthase -
  ABXT62_RS16315 (ABXT62_16325) - 3616307..3617173 (+) 867 WP_415356372.1 energy transducer TonB -
  ABXT62_RS16320 (ABXT62_16330) - 3617228..3617818 (+) 591 WP_415356373.1 YqgE/AlgH family protein -
  ABXT62_RS16325 (ABXT62_16335) ruvX 3617845..3618276 (+) 432 WP_415356374.1 Holliday junction resolvase RuvX -
  ABXT62_RS16330 (ABXT62_16340) pilU 3618289..3619428 (-) 1140 WP_415356375.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABXT62_RS16335 (ABXT62_16345) pilT 3619467..3620501 (-) 1035 WP_415356376.1 type IV pilus twitching motility protein PilT Machinery gene
  ABXT62_RS16340 (ABXT62_16350) - 3620627..3621319 (+) 693 WP_415356377.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABXT62_RS16345 (ABXT62_16355) proC 3621372..3622208 (+) 837 WP_415356378.1 pyrroline-5-carboxylate reductase -
  ABXT62_RS16350 (ABXT62_16360) - 3622225..3622812 (+) 588 WP_415356379.1 YggT family protein -
  ABXT62_RS16355 (ABXT62_16365) metX 3622823..3623971 (+) 1149 WP_415358235.1 homoserine O-succinyltransferase MetX -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42090.08 Da        Isoelectric Point: 5.8843

>NTDB_id=1016078 ABXT62_RS16330 WP_415356375.1 3618289..3619428(-) (pilU) [Halioglobus sp. Uisw_031]
MNIEDLLGRMVKEGASDGFITAGAPPSIKVDGEIHPIFDAPLTEQETRELVLSTLRKDQQEDFQKHHECNYAMSSESLGR
FRASAFMQRGKYGLVVRRIQSEIPTVDELGLPPIIKELAMTKRGLVIFVGATGTGKSTSLAAVVGYRNHNSRGHIISIED
PIEYIHEHAGCIITQREVGMDTESFDVALKNTLRQAPDVILIGEVRTAETMRQALTFAETGHLCLCTLHANNANQALDRI
QSFFPPEMHNQVWMDLSLNLKAMVAQQLLPHKDGKGRTPVVEVLLNSPLIAEYIRKGEVHLIKELMAKSTELGMQTLDQA
LVKAYKDGLIDKEEALRFADSANDVRLKIKMHERGEFGSGDGLGLAVDDQEEEGRFHGR

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1016078 ABXT62_RS16330 WP_415356375.1 3618289..3619428(-) (pilU) [Halioglobus sp. Uisw_031]
ATGAACATCGAAGACTTGCTGGGACGAATGGTGAAGGAGGGTGCATCTGACGGATTCATTACCGCGGGCGCTCCGCCCAG
CATCAAGGTTGATGGTGAAATCCATCCTATTTTCGACGCGCCACTTACTGAGCAGGAGACGCGCGAGCTTGTATTGTCGA
CTCTGCGAAAAGATCAGCAGGAAGATTTTCAGAAGCACCATGAATGTAATTACGCGATGAGCTCCGAAAGTCTGGGTCGT
TTTCGAGCCAGCGCTTTTATGCAACGTGGGAAATATGGCTTGGTAGTCCGGCGCATTCAGTCCGAAATACCGACTGTTGA
TGAATTGGGCCTGCCGCCCATTATCAAGGAGCTGGCCATGACCAAGCGTGGTCTGGTTATTTTTGTGGGAGCTACCGGCA
CAGGTAAATCCACTTCCCTCGCGGCGGTAGTCGGTTATCGCAACCACAATAGTCGCGGACACATTATTAGCATTGAAGAC
CCCATTGAATATATTCATGAGCACGCTGGCTGCATCATTACCCAGCGGGAAGTCGGTATGGACACGGAGTCTTTTGACGT
GGCCCTGAAAAACACCCTGCGACAAGCACCTGACGTCATATTGATTGGTGAGGTGCGGACGGCTGAAACCATGCGGCAGG
CTTTGACCTTTGCCGAGACCGGCCACCTCTGCCTATGCACCCTGCATGCAAACAATGCCAACCAGGCGCTGGATAGAATC
CAGAGTTTCTTCCCGCCTGAAATGCACAATCAGGTGTGGATGGACTTGTCCTTGAACCTGAAGGCAATGGTGGCTCAGCA
GTTACTGCCGCACAAGGACGGCAAGGGGCGCACTCCGGTCGTTGAGGTGTTGCTCAACTCGCCTTTGATCGCGGAATATA
TTCGCAAAGGTGAGGTGCACTTGATTAAGGAGCTGATGGCCAAGTCAACTGAGCTCGGCATGCAGACACTCGACCAGGCG
CTGGTGAAAGCTTACAAGGATGGACTAATAGATAAGGAGGAAGCTTTACGCTTCGCCGATTCTGCCAACGATGTGCGCCT
GAAAATCAAAATGCACGAGAGAGGCGAGTTTGGATCAGGCGATGGGTTGGGGCTTGCCGTCGATGACCAAGAGGAGGAAG
GCCGTTTCCACGGACGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.527

92.612

0.607
  pilU Acinetobacter baylyi ADP1

58.989

93.931

0.554
  pilU Vibrio cholerae strain A1552

53.804

97.098

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.165

92.612

0.39
  pilT Legionella pneumophila strain Lp02

41.194

88.391

0.364
  pilT Legionella pneumophila strain ERS1305867

41.194

88.391

0.364
  pilT Acinetobacter baylyi ADP1

40.469

89.974

0.364

Multiple sequence alignment