Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABV408_RS01520 Genome accession   NZ_CP159578
Coordinates   331731..332771 (+) Length   346 a.a.
NCBI ID   WP_353980787.1    Uniprot ID   A0AB74U843
Organism   Salinicola endophyticus strain HNIBRBA4755     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 326731..337771
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABV408_RS01490 (ABV408_01490) sfsA 326866..327636 (+) 771 WP_353980782.1 DNA/RNA nuclease SfsA -
  ABV408_RS01495 (ABV408_01495) metW 327644..328240 (-) 597 WP_353980783.1 methionine biosynthesis protein MetW -
  ABV408_RS01500 (ABV408_01500) - 328240..329397 (-) 1158 WP_353980784.1 homoserine O-acetyltransferase -
  ABV408_RS01505 (ABV408_01505) - 329478..330068 (-) 591 WP_207036164.1 YggT family protein -
  ABV408_RS01510 (ABV408_01510) proC 330097..330936 (-) 840 WP_353980785.1 pyrroline-5-carboxylate reductase -
  ABV408_RS01515 (ABV408_01515) - 330974..331690 (-) 717 WP_353980786.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABV408_RS01520 (ABV408_01520) pilT 331731..332771 (+) 1041 WP_353980787.1 type IV pilus twitching motility protein PilT Machinery gene
  ABV408_RS01525 (ABV408_01525) pilU 332865..334004 (+) 1140 WP_353980788.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABV408_RS01530 (ABV408_01530) tyrS 334097..335296 (+) 1200 WP_353980789.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 37940.39 Da        Isoelectric Point: 6.5093

>NTDB_id=1014600 ABV408_RS01520 WP_353980787.1 331731..332771(+) (pilT) [Salinicola endophyticus strain HNIBRBA4755]
MDITELLAFSAKQDASDLHLSAGLPPMIRVDGDMRRLNVPALDDSQVRRLIYDVMNDRQRRDFEADLEADFSFEVNGVAR
FRVNVFTQGRGAGAVFRVIPSLVRSLEELDLGEVFRRLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINSHRQEHILTIED
PIEFVHQSKRCLINQREVHRDTKGFAPALRSALREDPDVILVGELRDLETVRLALTAAETGHLVFATLHTTSAAKTIDRI
IDVFPGEEKGMVRSMLSESLQGVISQTLLKRAGEGGGRVAAHEILVANSAIRNLIREGKVAQMYSAMQTGGNLGMQTLDT
ALARLIADGVVSRQEAQAKSRGGFEG

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1014600 ABV408_RS01520 WP_353980787.1 331731..332771(+) (pilT) [Salinicola endophyticus strain HNIBRBA4755]
ATGGATATTACCGAACTGCTGGCGTTCTCGGCAAAGCAAGACGCCTCGGACCTGCACCTCTCGGCGGGTCTGCCGCCGAT
GATTCGCGTCGATGGCGACATGCGGCGCCTCAATGTGCCAGCGCTCGACGATAGCCAGGTTCGGCGGCTTATCTACGATG
TGATGAACGATCGTCAGCGGCGCGACTTCGAGGCCGATCTGGAAGCCGATTTCTCGTTCGAGGTGAACGGGGTCGCTCGC
TTCCGGGTCAATGTCTTCACCCAGGGGCGCGGTGCCGGCGCGGTGTTCCGAGTCATTCCCAGCCTCGTGCGCTCGCTCGA
GGAGCTCGATCTGGGCGAGGTGTTCCGCCGCCTGGCGATGCTGCCGCGGGGCCTGGTGCTGGTCACCGGCCCGACCGGTT
CGGGCAAGAGCACCACGCTGGCGGCGATGATCGACTACATCAACAGTCACCGCCAGGAGCATATTCTCACCATCGAGGAC
CCGATCGAGTTCGTCCACCAGAGCAAGCGCTGTCTGATCAACCAGCGTGAGGTGCATCGTGACACCAAGGGTTTCGCGCC
AGCGCTACGCTCGGCGCTGCGCGAGGACCCCGATGTGATCCTGGTCGGAGAGCTGCGTGACCTGGAGACGGTGCGTCTGG
CGCTGACCGCCGCCGAGACCGGGCATCTGGTCTTCGCCACCCTGCACACGACCTCGGCGGCCAAGACCATCGATCGCATC
ATCGATGTCTTCCCCGGAGAGGAGAAGGGTATGGTGCGGTCGATGCTGTCTGAGTCACTGCAGGGCGTGATCTCGCAGAC
GCTGCTCAAGCGTGCAGGTGAGGGTGGGGGGCGTGTCGCGGCGCACGAGATCCTCGTCGCCAACTCGGCGATCCGCAATC
TGATCCGCGAAGGCAAGGTGGCACAGATGTACTCGGCGATGCAGACCGGCGGCAATCTGGGCATGCAGACCCTGGATACG
GCGCTCGCCCGACTGATCGCCGATGGCGTGGTGTCGCGTCAGGAGGCACAGGCCAAATCCCGAGGGGGATTCGAGGGCTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

76.246

98.555

0.751
  pilT Pseudomonas stutzeri DSM 10701

75.953

98.555

0.749
  pilT Acinetobacter baylyi ADP1

74.78

98.555

0.737
  pilT Acinetobacter baumannii D1279779

74.194

98.555

0.731
  pilT Acinetobacter baumannii strain A118

74.194

98.555

0.731
  pilT Acinetobacter nosocomialis M2

73.9

98.555

0.728
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.732

97.977

0.673
  pilT Vibrio cholerae strain A1552

68.732

97.977

0.673
  pilT Legionella pneumophila strain Lp02

68.249

97.399

0.665
  pilT Legionella pneumophila strain ERS1305867

68.249

97.399

0.665
  pilT Neisseria meningitidis 8013

64.912

98.844

0.642
  pilT Neisseria gonorrhoeae MS11

64.62

98.844

0.639
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.521

94.22

0.523
  pilU Vibrio cholerae strain A1552

44.345

97.11

0.431
  pilU Pseudomonas stutzeri DSM 10701

41.667

97.11

0.405
  pilU Acinetobacter baylyi ADP1

41.003

97.977

0.402

Multiple sequence alignment