Detailed information NTG1014601

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABV408_RS01525
• Genome accession: NZ_CP159578
• Coordinates: 332865..334004 (+)
• Length: 379 a.a.
• NCBI ID: WP_353980788.1
• Uniprot ID: A0AB74UFS3
• Organism: Salinicola endophyticus strain HNIBRBA4755
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 327865..339004

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABV408_RS01500 (ABV408_01500)	-	328240..329397 (-)	1158	WP_353980784.1	homoserine O-acetyltransferase	-
ABV408_RS01505 (ABV408_01505)	-	329478..330068 (-)	591	WP_207036164.1	YggT family protein	-
ABV408_RS01510 (ABV408_01510)	proC	330097..330936 (-)	840	WP_353980785.1	pyrroline-5-carboxylate reductase	-
ABV408_RS01515 (ABV408_01515)	-	330974..331690 (-)	717	WP_353980786.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABV408_RS01520 (ABV408_01520)	pilT	331731..332771 (+)	1041	WP_353980787.1	type IV pilus twitching motility protein PilT	Machinery gene
ABV408_RS01525 (ABV408_01525)	pilU	332865..334004 (+)	1140	WP_353980788.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ABV408_RS01530 (ABV408_01530)	tyrS	334097..335296 (+)	1200	WP_353980789.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 379 a.a.
• Molecular weight: 41713.63 Da
• Isoelectric Point: 5.4524

>NTDB_id=1014601 ABV408_RS01525 WP_353980788.1 332865..334004(+) (pilU) [Salinicola endophyticus strain HNIBRBA4755]
MTPHQWFEQLLDIMIAKQASDLLISTGAPPCLKLDGKLVALGDVPLRPEQVRELVATTLPQGGREDFEREREANFALSLS
GKGRFRVSAFFQRSQQAMVVRRIAYDIPALESLGLPEAVASLPTIRRGLVLVVGGTGSGKSTTLASMIQHRNRTSGGHII
CIEDPIEYMHPHQRGIINQREVGIDTESYEIGLRNALRQAPDVIVIGEIRSRDTMELALAFAETGHLCLATLHANNADQA
VERILNLFPAERHDQIWMDLSLNLRAVVAQQLLPRRDGEGRCLATEVMLQSPLISELIRRGEISELKPLIRRSRELGMQT
FDQSLYALYSQGLIGEHVALSHADSANDLRMLIKYGEAGASGEVGGEAGEGQFTLADDD

Nucleotide

• Length: 1140 bp

>NTDB_id=1014601 ABV408_RS01525 WP_353980788.1 332865..334004(+) (pilU) [Salinicola endophyticus strain HNIBRBA4755]
ATGACGCCGCACCAATGGTTTGAACAGCTTCTCGATATCATGATTGCCAAGCAGGCCTCTGACCTGCTGATTTCCACCGG
CGCGCCGCCTTGTCTCAAGCTCGACGGCAAGCTGGTCGCGCTGGGTGATGTGCCGCTGCGACCCGAACAGGTGCGTGAGC
TGGTGGCGACCACGCTGCCCCAGGGAGGGCGCGAGGATTTCGAGCGCGAGCGCGAGGCCAACTTTGCGCTCAGCCTGAGC
GGCAAGGGGCGGTTCCGGGTCAGCGCCTTCTTCCAGCGCAGCCAGCAGGCGATGGTGGTGCGCCGCATCGCCTATGACAT
CCCGGCACTCGAGAGTCTGGGGCTACCCGAGGCGGTGGCCTCGCTGCCGACCATCCGACGCGGTTTGGTACTGGTGGTCG
GGGGAACCGGCAGCGGCAAGTCGACCACCCTGGCGTCGATGATCCAGCACCGCAACCGCACCAGCGGCGGACATATCATC
TGCATCGAAGACCCGATCGAGTACATGCATCCGCATCAGCGCGGGATTATCAATCAGCGCGAGGTGGGCATCGACACCGA
GTCCTACGAGATCGGACTCAGGAATGCGCTGCGTCAGGCGCCGGACGTGATCGTGATCGGTGAGATCCGCAGCCGCGACA
CCATGGAGCTGGCGCTCGCCTTCGCCGAGACCGGGCATCTGTGTCTCGCCACGCTCCACGCCAACAATGCCGACCAGGCG
GTGGAGCGGATCCTCAATCTGTTCCCGGCCGAGCGCCATGACCAGATATGGATGGATCTGTCGCTCAACCTGCGCGCGGT
CGTGGCCCAGCAGCTGCTGCCGCGGCGCGATGGCGAGGGGCGCTGTCTGGCCACCGAAGTGATGCTGCAGTCGCCGCTGA
TCAGTGAGCTGATCCGGCGTGGCGAGATCAGTGAACTCAAGCCGCTGATTCGGCGCTCGCGGGAGCTGGGCATGCAGACC
TTCGACCAGTCGCTCTACGCTCTCTATAGCCAGGGCCTGATCGGCGAGCACGTTGCGCTGTCGCACGCCGACTCCGCCAA
CGATCTGCGCATGCTGATCAAGTATGGCGAGGCCGGCGCATCGGGTGAGGTCGGCGGCGAGGCTGGAGAAGGCCAGTTCA
CTCTGGCCGATGACGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (41-274)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.15	98.681	0.554
pilU	Vibrio cholerae strain A1552	56.034	91.821	0.515
pilU	Acinetobacter baylyi ADP1	53.977	92.876	0.501
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.646	95.515	0.417