Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABVN21_RS24970 Genome accession   NZ_CP159258
Coordinates   5522957..5523991 (+) Length   344 a.a.
NCBI ID   WP_339556558.1    Uniprot ID   A0AAU8E1I4
Organism   Pseudomonas sp. MYb327     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5517957..5528991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABVN21_RS24940 (ABVN21_24940) - 5518255..5518689 (-) 435 WP_339556552.1 DUF4426 domain-containing protein -
  ABVN21_RS24945 (ABVN21_24945) metW 5518716..5519336 (-) 621 WP_339556553.1 methionine biosynthesis protein MetW -
  ABVN21_RS24950 (ABVN21_24950) - 5519344..5520483 (-) 1140 WP_339556554.1 homoserine O-acetyltransferase -
  ABVN21_RS24955 (ABVN21_24955) - 5520736..5521323 (-) 588 WP_339556555.1 YggT family protein -
  ABVN21_RS24960 (ABVN21_24960) proC 5521348..5522166 (-) 819 WP_339556556.1 pyrroline-5-carboxylate reductase -
  ABVN21_RS24965 (ABVN21_24965) - 5522202..5522900 (-) 699 WP_339556557.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABVN21_RS24970 (ABVN21_24970) pilT 5522957..5523991 (+) 1035 WP_339556558.1 type IV pilus twitching motility protein PilT Machinery gene
  ABVN21_RS24975 (ABVN21_24975) - 5524129..5524746 (-) 618 WP_339556559.1 C40 family peptidase -
  ABVN21_RS24980 (ABVN21_24980) - 5524996..5525427 (+) 432 WP_339556560.1 TM2 domain-containing protein -
  ABVN21_RS24985 (ABVN21_24985) - 5525475..5526746 (-) 1272 WP_339556561.1 dihydroorotase -
  ABVN21_RS24990 (ABVN21_24990) - 5526743..5527747 (-) 1005 WP_007939362.1 aspartate carbamoyltransferase catalytic subunit -
  ABVN21_RS24995 (ABVN21_24995) pyrR 5527775..5528278 (-) 504 WP_034147547.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ABVN21_RS25000 (ABVN21_25000) ruvX 5528373..5528810 (-) 438 WP_034147546.1 Holliday junction resolvase RuvX -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37869.44 Da        Isoelectric Point: 7.0209

>NTDB_id=1012944 ABVN21_RS24970 WP_339556558.1 5522957..5523991(+) (pilT) [Pseudomonas sp. MYb327]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKEVQALIYDIMNDTQRVDFEKHLETDFSFEVPGVAR
FRVNAFNQSRGAGAVFRTIPSRVLSMDELGMGDVFRKMTDAPRGLVLVTGPTGSGKSTTLAAMIDHLSNHRHHHILTIED
PIEFVHESRKCLINQREVHRDTRSFATALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPGDEKSMVRSMLSESLLAVVSQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQAGGSLGMQTLDMGL
KDLVTKGLVSREHAREKARSPDSF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1012944 ABVN21_RS24970 WP_339556558.1 5522957..5523991(+) (pilT) [Pseudomonas sp. MYb327]
ATGGATATCACTGAGCTGCTGGCGTTCAGCGCAAAACAAGGAGCTTCCGATCTGCATCTGTCCGCCGGCTTGCCACCGAT
GATTCGCGTCGATGGCGATGTTCGGCGGATCAATCTGCCGGCCCTGGACCACAAAGAAGTGCAGGCGCTGATCTACGACA
TCATGAACGACACCCAGCGGGTCGACTTCGAAAAGCATCTGGAAACCGACTTTTCCTTTGAAGTGCCCGGCGTGGCACGC
TTTCGGGTCAATGCGTTCAACCAGAGTCGCGGTGCTGGTGCGGTCTTCCGTACGATTCCCTCCAGAGTCCTGAGCATGGA
TGAACTGGGGATGGGCGATGTGTTTCGCAAGATGACCGATGCGCCCCGGGGGCTGGTGCTGGTGACCGGCCCCACCGGTT
CCGGCAAGTCCACGACGCTGGCGGCGATGATCGATCATCTGAGCAACCATCGCCATCACCATATCCTCACGATCGAAGAC
CCCATCGAGTTCGTTCACGAATCACGCAAATGCCTGATCAATCAGCGCGAGGTGCATCGCGACACCCGCAGCTTCGCCAC
GGCGCTGCGTTCGGCCCTGCGGGAAGATCCGGACGTGATCCTGGTCGGAGAGATGCGCGATCTGGAAACCATTCGCCTGG
CCCTGACTGCCGCTGAAACCGGTCACTTGGTGTTCGGCACGCTGCACACCACATCGGCGGCGAAAACCATCGACCGGATC
GTGGACGTGTTTCCAGGTGATGAGAAATCGATGGTGCGGTCGATGCTTTCCGAGTCGTTGCTGGCGGTGGTGTCCCAGAC
GCTGGTAAAGAAAATCGGCGGCGGACGCATTGCTGCCCACGAGATCATGCTCGGGACTTCGGCGATTCGTAACCTTATCC
GCGAAGACAAGGTGGCGCAGATGTACTCATCGATTCAGGCAGGCGGATCGCTGGGGATGCAGACGCTGGATATGGGCTTG
AAGGACTTGGTGACCAAAGGGCTGGTCAGTCGTGAGCACGCGCGGGAGAAGGCGCGCTCACCGGACAGTTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

84.593

100

0.846
  pilT Pseudomonas aeruginosa PAK

84.593

100

0.846
  pilT Acinetobacter baylyi ADP1

77.326

100

0.773
  pilT Acinetobacter baumannii D1279779

76.453

100

0.765
  pilT Acinetobacter baumannii strain A118

76.453

100

0.765
  pilT Acinetobacter nosocomialis M2

76.453

100

0.765
  pilT Legionella pneumophila strain Lp02

70.93

100

0.709
  pilT Legionella pneumophila strain ERS1305867

70.93

100

0.709
  pilT Neisseria meningitidis 8013

65.507

100

0.657
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.062

97.965

0.657
  pilT Vibrio cholerae strain A1552

67.062

97.965

0.657
  pilT Neisseria gonorrhoeae MS11

65.217

100

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.014

100

0.512
  pilU Vibrio cholerae strain A1552

40

100

0.401
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.299

97.384

0.392

Multiple sequence alignment