Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V8F66_RS01725 Genome accession   NZ_CP158484
Coordinates   354157..355200 (+) Length   347 a.a.
NCBI ID   WP_035562773.1    Uniprot ID   A0A0D5M885
Organism   Vreelandella sp. SM1641     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 349157..360200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8F66_RS01695 (V8F66_01690) - 349489..349938 (-) 450 WP_179916749.1 DUF4426 domain-containing protein -
  V8F66_RS01700 (V8F66_01695) metW 349980..350573 (-) 594 WP_179916775.1 methionine biosynthesis protein MetW -
  V8F66_RS01705 (V8F66_01700) - 350573..351799 (-) 1227 WP_179916748.1 homoserine O-acetyltransferase -
  V8F66_RS01710 (V8F66_01705) - 351866..352459 (-) 594 WP_035562766.1 YggT family protein -
  V8F66_RS01715 (V8F66_01710) proC 352529..353353 (-) 825 WP_035562768.1 pyrroline-5-carboxylate reductase -
  V8F66_RS01720 (V8F66_01715) - 353408..354124 (-) 717 WP_179916747.1 YggS family pyridoxal phosphate-dependent enzyme -
  V8F66_RS01725 (V8F66_01720) pilT 354157..355200 (+) 1044 WP_035562773.1 type IV pilus twitching motility protein PilT Machinery gene
  V8F66_RS01730 (V8F66_01725) pilU 355208..356368 (+) 1161 WP_179916746.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V8F66_RS01735 (V8F66_01730) - 356405..357544 (-) 1140 WP_035562776.1 anhydro-N-acetylmuramic acid kinase -
  V8F66_RS01740 (V8F66_01735) tyrS 357690..358889 (+) 1200 WP_179916745.1 tyrosine--tRNA ligase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38248.73 Da        Isoelectric Point: 6.1859

>NTDB_id=1011494 V8F66_RS01725 WP_035562773.1 354157..355200(+) (pilT) [Vreelandella sp. SM1641]
MDITELLAFSAKQNASDLHLSAGLPPMIRVDGDIRRLNVPAIDNNDVRKLIYDIMNDRQRRDYEEHLEIDFSFEVPGVAR
FRVNAFTQARGAGAVFRTIPSQVLSMQTLGLGEVFERLAMLPRGLVLVTGPTGSGKSTTLAAMIDYINDHRYEHILTIED
PVEFVHASKRCLINQREVHRDTHSFADALRSALREDPDVILVGELRDLETIRLALTAAETGHLVLGTLHTTSAAKTVDRI
IDVFPGEEKAMVRSMLSESLQAVVSQTLLKREGGGRVAAHEILIATSAVRNLIREDKVAQMYSAIQTGGSLGMQTLDSAL
SRLVKEGSVSLQEAQLRSKGTLSIKEE

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1011494 V8F66_RS01725 WP_035562773.1 354157..355200(+) (pilT) [Vreelandella sp. SM1641]
ATGGATATTACCGAACTGCTAGCATTCTCGGCAAAGCAGAATGCCTCTGACTTGCACCTTTCGGCCGGTTTGCCGCCCAT
GATACGTGTTGATGGCGACATTCGTCGGCTCAATGTGCCTGCTATCGATAATAACGATGTGCGCAAATTGATCTACGACA
TCATGAATGATCGACAGCGGCGCGACTACGAAGAGCATCTGGAAATTGATTTTTCCTTTGAGGTGCCGGGGGTGGCGCGC
TTCCGCGTGAATGCCTTCACCCAAGCGCGCGGTGCAGGGGCGGTGTTTCGCACCATCCCCAGTCAAGTGCTCTCCATGCA
GACCTTGGGGCTAGGCGAGGTGTTTGAGCGCTTAGCGATGTTGCCGCGGGGCTTAGTGCTGGTGACGGGGCCAACGGGCT
CGGGCAAGAGCACCACGCTGGCCGCGATGATCGATTACATTAACGATCATCGTTATGAGCATATTCTCACTATTGAAGAC
CCCGTCGAGTTTGTCCACGCCAGCAAGCGCTGTTTGATTAATCAGCGCGAAGTGCACCGTGACACCCATAGCTTTGCCGA
TGCGCTGCGCAGCGCGCTGCGTGAAGACCCGGATGTGATTCTAGTCGGTGAGCTGCGTGATCTTGAAACTATCCGCTTGG
CGTTGACCGCTGCGGAAACAGGGCATTTGGTACTGGGGACGCTACACACCACTTCGGCAGCTAAAACCGTTGACCGGATT
ATCGATGTCTTTCCCGGTGAAGAGAAAGCCATGGTGCGCTCCATGCTCTCTGAATCACTCCAGGCAGTGGTGTCACAAAC
CCTGCTTAAACGTGAGGGCGGTGGGCGCGTGGCGGCCCACGAAATCCTTATTGCCACGTCTGCAGTGCGCAACTTGATTC
GTGAAGACAAAGTGGCGCAGATGTACTCGGCCATTCAAACCGGTGGCAGCTTGGGCATGCAAACGCTGGATTCCGCGCTG
TCCCGGTTAGTGAAAGAGGGTTCGGTAAGTTTGCAAGAAGCGCAGCTGAGGTCAAAGGGAACGCTGTCGATAAAGGAGGA
GTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D5M885

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.171

97.695

0.764
  pilT Pseudomonas aeruginosa PAK

78.171

97.695

0.764
  pilT Acinetobacter nosocomialis M2

76.401

97.695

0.746
  pilT Acinetobacter baumannii D1279779

76.401

97.695

0.746
  pilT Acinetobacter baumannii strain A118

76.401

97.695

0.746
  pilT Acinetobacter baylyi ADP1

75.516

97.695

0.738
  pilT Legionella pneumophila strain Lp02

72.239

96.542

0.697
  pilT Legionella pneumophila strain ERS1305867

72.239

96.542

0.697
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.565

99.424

0.692
  pilT Vibrio cholerae strain A1552

69.565

99.424

0.692
  pilT Neisseria meningitidis 8013

65.507

99.424

0.651
  pilT Neisseria gonorrhoeae MS11

65.217

99.424

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

55.215

93.948

0.519
  pilU Pseudomonas stutzeri DSM 10701

41.888

97.695

0.409
  pilU Vibrio cholerae strain A1552

41.493

96.542

0.401
  pilU Acinetobacter baylyi ADP1

40.774

96.83

0.395

Multiple sequence alignment