Detailed information NTG1011495

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: V8F66_RS01730
• Genome accession: NZ_CP158484
• Coordinates: 355208..356368 (+)
• Length: 386 a.a.
• NCBI ID: WP_179916746.1
• Uniprot ID: A0A7Z0LVG2
• Organism: Vreelandella sp. SM1641
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 350208..361368

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
V8F66_RS01705 (V8F66_01700)	-	350573..351799 (-)	1227	WP_179916748.1	homoserine O-acetyltransferase	-
V8F66_RS01710 (V8F66_01705)	-	351866..352459 (-)	594	WP_035562766.1	YggT family protein	-
V8F66_RS01715 (V8F66_01710)	proC	352529..353353 (-)	825	WP_035562768.1	pyrroline-5-carboxylate reductase	-
V8F66_RS01720 (V8F66_01715)	-	353408..354124 (-)	717	WP_179916747.1	YggS family pyridoxal phosphate-dependent enzyme	-
V8F66_RS01725 (V8F66_01720)	pilT	354157..355200 (+)	1044	WP_035562773.1	type IV pilus twitching motility protein PilT	Machinery gene
V8F66_RS01730 (V8F66_01725)	pilU	355208..356368 (+)	1161	WP_179916746.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
V8F66_RS01735 (V8F66_01730)	-	356405..357544 (-)	1140	WP_035562776.1	anhydro-N-acetylmuramic acid kinase	-
V8F66_RS01740 (V8F66_01735)	tyrS	357690..358889 (+)	1200	WP_179916745.1	tyrosine--tRNA ligase	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 42686.83 Da
• Isoelectric Point: 6.2243

>NTDB_id=1011495 V8F66_RS01730 WP_179916746.1 355208..356368(+) (pilU) [Vreelandella sp. SM1641]
MSQDAQLSPAQWLHQLLDIMVEQNASDLLISVGAPPSLKMPDGLVPLGQQRLTAHQVSELVTHALPEGLRERFTCEHEAN
FALSREGKWRFRVSAFQQRNQMAMVVRSIAMEIPCLEALGVPEQLAELAQAKRGLVLVVGGTGTGKSTTLAAMIQQRNET
VGGHIISIEDPIEYLHSHKRAIVNQREVGIDTESFEVALKNTLRQAPDVILIGEIRTRETMEHALTFAETGHLCLATLHA
NNANQALERIIHFFPHERHEQIWMDLSLNLRAVVAQQLLPTLAGGRCAAIEIMLQSPRIADLIRKGEVAEMKAAMAKSRD
AGMQTFDQALHDLHQAGHISREVAMAHADSANDLRMLLNVSDAKGRNLSLDEQVPSHLSLRDGDDY

Nucleotide

• Length: 1161 bp

>NTDB_id=1011495 V8F66_RS01730 WP_179916746.1 355208..356368(+) (pilU) [Vreelandella sp. SM1641]
ATGAGTCAAGATGCCCAGCTTTCGCCCGCCCAGTGGCTGCACCAGCTGCTTGATATTATGGTTGAGCAAAACGCTTCTGA
TTTGCTCATCTCCGTGGGTGCGCCGCCAAGCTTGAAAATGCCCGATGGGTTGGTGCCCTTGGGTCAGCAACGCTTAACCG
CGCATCAGGTAAGTGAGCTGGTTACTCACGCTCTACCGGAAGGATTGCGTGAACGTTTTACCTGTGAGCATGAAGCGAAT
TTCGCGCTCAGCCGAGAAGGCAAGTGGCGCTTTCGGGTCAGCGCGTTTCAGCAGCGCAATCAGATGGCCATGGTAGTGCG
CAGCATCGCGATGGAAATTCCTTGCCTAGAGGCGCTGGGTGTACCAGAACAGTTGGCAGAATTAGCTCAGGCCAAACGGG
GGCTGGTACTGGTGGTGGGTGGCACGGGAACGGGTAAGTCGACGACCCTGGCGGCGATGATTCAGCAGCGCAACGAAACC
GTTGGCGGCCATATCATCAGCATCGAAGACCCCATTGAGTACTTGCACTCGCACAAGCGCGCTATCGTCAATCAGCGCGA
GGTGGGGATCGATACCGAATCGTTTGAGGTGGCGCTAAAAAATACCCTGCGCCAGGCGCCGGATGTGATTCTGATTGGTG
AAATCCGCACCCGAGAAACCATGGAACACGCACTGACCTTTGCTGAAACCGGCCATCTCTGCCTGGCAACGCTACATGCC
AATAACGCCAATCAGGCGTTAGAGCGCATCATTCACTTCTTCCCTCATGAACGCCATGAGCAGATATGGATGGATCTGTC
TCTGAATCTGCGTGCAGTCGTCGCCCAGCAGCTGCTGCCCACGCTTGCAGGCGGGCGCTGTGCTGCTATCGAAATCATGC
TGCAGTCGCCGCGCATCGCCGACTTGATCCGTAAAGGGGAGGTCGCCGAGATGAAAGCAGCGATGGCCAAGTCCCGCGAT
GCCGGAATGCAGACCTTCGATCAGGCGCTGCATGATCTGCATCAGGCGGGGCATATCAGCAGAGAGGTGGCCATGGCACA
CGCCGACTCGGCTAACGACCTGCGCATGCTGCTCAATGTTAGTGACGCCAAGGGGCGTAACCTCTCGCTAGATGAACAGG
TGCCGAGTCATTTAAGCCTACGCGATGGCGATGATTACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (78-280)

3D structure

• AlphaFold DB: A0A7Z0LVG2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.667	97.15	0.57
pilU	Vibrio cholerae strain A1552	57.714	90.674	0.523
pilU	Acinetobacter baylyi ADP1	54.121	94.301	0.51
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.11	94.301	0.378
pilT	Legionella pneumophila strain Lp02	42.727	85.492	0.365
pilT	Legionella pneumophila strain ERS1305867	42.727	85.492	0.365
pilT	Pseudomonas aeruginosa PAK	41.667	87.047	0.363
pilT	Pseudomonas stutzeri DSM 10701	41.369	87.047	0.36