Detailed information NTG1011221

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: ABS744_RS04930
• Genome accession: NZ_CP158446
• Coordinates: 994659..995639 (+)
• Length: 326 a.a.
• NCBI ID: WP_001327409.1
• Uniprot ID: A0A3T4X286
• Organism: Escherichia coli strain CFT073
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 989659..1000639

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABS744_RS04900 (ABS744_04895)	yggM	990260..991267 (+)	1008	WP_000745192.1	DUF1202 family protein	-
ABS744_RS04905 (ABS744_04900)	hemW	991336..992472 (-)	1137	WP_000239981.1	radical SAM family heme chaperone HemW	-
ABS744_RS04910 (ABS744_04905)	rdgB	992465..993058 (-)	594	WP_001174738.1	XTP/dITP diphosphatase	-
ABS744_RS04915 (ABS744_04910)	yggU	993066..993356 (-)	291	WP_001277222.1	DUF167 family protein YggU	-
ABS744_RS04920 (ABS744_04915)	yggT	993353..993919 (-)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
ABS744_RS04925 (ABS744_04920)	yggS	993937..994641 (-)	705	WP_000997795.1	pyridoxal phosphate homeostasis protein	-
ABS744_RS04930 (ABS744_04925)	pilT	994659..995639 (+)	981	WP_001327409.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ABS744_RS04935 (ABS744_04930)	ruvX	995813..996229 (-)	417	WP_000017106.1	Holliday junction resolvase RuvX	-
ABS744_RS04940 (ABS744_04935)	yqgE	996229..996792 (-)	564	WP_001053178.1	YqgE/AlgH family protein	-
ABS744_RS04945 (ABS744_04940)	gshB	996901..997851 (-)	951	WP_000593261.1	glutathione synthase	-
ABS744_RS04950 (ABS744_04945)	rsmE	997864..998595 (-)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
ABS744_RS04955 (ABS744_04950)	endA	998675..999382 (-)	708	WP_001327408.1	deoxyribonuclease I	-
ABS744_RS04960 (ABS744_04955)	yggI	999477..999974 (-)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 36089.34 Da
• Isoelectric Point: 5.9975

>NTDB_id=1011221 ABS744_RS04930 WP_001327409.1 994659..995639(+) (pilT) [Escherichia coli strain CFT073]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDALDVEELLREWLDDDQRTILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VKEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1011221 ABS744_RS04930 WP_001327409.1 994659..995639(+) (pilT) [Escherichia coli strain CFT073]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCTGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGACAATATTGCTGGAGAATGGTCAGTTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATACCAGTCAGCGATGTTTGATCCAACAGCGGGAGATTGGTTTGCACTGTATGACTTTCGCATCGGGATTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGATAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCG
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGAAAGAAGGGCGCTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

3D structure

• AlphaFold DB: A0A3T4X286

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	49.541	100	0.497
pilT	Vibrio cholerae strain A1552	49.541	100	0.497
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii D1279779	46.789	100	0.469
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter nosocomialis M2	46.789	100	0.469
pilT	Acinetobacter baumannii strain A118	46.789	100	0.469
pilT	Pseudomonas stutzeri DSM 10701	46.483	100	0.466
pilT	Pseudomonas aeruginosa PAK	46.177	100	0.463
pilT	Legionella pneumophila strain Lp02	45.26	100	0.454
pilT	Legionella pneumophila strain ERS1305867	45.26	100	0.454
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.138	97.546	0.411
pilU	Vibrio cholerae strain A1552	40.379	97.239	0.393
pilU	Pseudomonas stutzeri DSM 10701	37.576	100	0.38
pilU	Acinetobacter baylyi ADP1	36.957	98.773	0.365
pilB	Legionella pneumophila strain ERS1305867	31.152	100	0.365