Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABHF91_RS14235 Genome accession   NZ_CP158016
Coordinates   3030632..3031666 (-) Length   344 a.a.
NCBI ID   WP_409420918.1    Uniprot ID   -
Organism   Pseudaeromonas sp. ZJS20     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3025632..3036666
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABHF91_RS14215 (ABHF91_14200) - 3026518..3027225 (-) 708 WP_409420914.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ABHF91_RS14220 (ABHF91_14205) srmB 3027310..3028614 (+) 1305 WP_409420915.1 ATP-dependent RNA helicase SrmB -
  ABHF91_RS14225 (ABHF91_14210) yaaA 3028696..3029469 (-) 774 WP_409420916.1 peroxide stress protein YaaA -
  ABHF91_RS14230 (ABHF91_14215) pilU 3029515..3030621 (-) 1107 WP_409420917.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABHF91_RS14235 (ABHF91_14220) pilT 3030632..3031666 (-) 1035 WP_409420918.1 type IVa pilus ATPase TapT Machinery gene
  ABHF91_RS14240 (ABHF91_14225) - 3031704..3032411 (+) 708 WP_409420919.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABHF91_RS14245 (ABHF91_14230) proC 3032412..3033236 (+) 825 WP_409420920.1 pyrroline-5-carboxylate reductase -
  ABHF91_RS14250 (ABHF91_14235) - 3033245..3033796 (+) 552 WP_409420921.1 YggT family protein -
  ABHF91_RS14255 (ABHF91_14240) yggU 3033796..3034089 (+) 294 WP_409420922.1 DUF167 family protein YggU -
  ABHF91_RS14260 (ABHF91_14245) - 3034177..3035799 (+) 1623 WP_409420923.1 hypothetical protein -
  ABHF91_RS14265 (ABHF91_14250) rdgB 3035873..3036469 (+) 597 WP_409420924.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38189.16 Da        Isoelectric Point: 6.7820

>NTDB_id=1009524 ABHF91_RS14235 WP_409420918.1 3030632..3031666(-) (pilT) [Pseudaeromonas sp. ZJS20]
MDITELLAFSVKHKASDLHLSAGVPPMIRVDGDVRKINLPPLEHRQVHTLVYDIMNDHQRKELEENLEVDFSFEVPNLAR
FRVNAFHQSRGVAAVFRTIPSKVLTLDDLNAPEIFRKICEYPRGLVLVTGPTGSGKSTTLAGMIDYINDGFHHHILTIED
PIEFVHENKLCLVNQREVHRDTLGFSAALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGAEKDMVRSMLSESLRAVISQTLLKKTGGGRVAAHEIMIGIPAIRNLIREDKIAQMYSVIQTGMMHGMQTMDQCL
KQLVARGVVNVADAKAKAVDPNSI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1009524 ABHF91_RS14235 WP_409420918.1 3030632..3031666(-) (pilT) [Pseudaeromonas sp. ZJS20]
ATGGATATCACAGAATTACTGGCCTTTAGTGTAAAACATAAAGCGTCGGATCTACACCTCTCCGCCGGCGTGCCACCCAT
GATCCGGGTGGATGGCGATGTCCGCAAGATCAACCTGCCACCCCTGGAGCACCGCCAGGTGCACACATTGGTGTATGACA
TCATGAATGACCACCAGCGCAAGGAGCTGGAGGAAAATCTGGAGGTGGACTTCTCCTTCGAGGTGCCCAACCTGGCCCGC
TTCCGTGTGAACGCCTTCCACCAGAGCCGCGGTGTGGCGGCGGTATTCCGGACCATCCCTAGCAAGGTGCTGACCCTGGA
TGACCTGAACGCGCCGGAGATCTTCCGCAAGATCTGCGAATACCCCCGGGGTCTGGTGCTGGTGACCGGGCCCACCGGCT
CCGGTAAGTCCACCACCTTGGCGGGCATGATCGATTACATCAACGACGGTTTCCACCACCACATCCTCACCATCGAGGAC
CCCATCGAATTCGTGCACGAGAACAAGCTGTGCCTGGTGAACCAGCGGGAAGTGCACCGGGATACCCTGGGCTTCAGCGC
CGCCCTGCGTTCGGCCCTGCGTGAAGACCCGGACATCATACTGGTGGGGGAAATGCGGGACCTGGAAACCATACGGCTGG
CCCTCACCGCGGCGGAAACCGGTCACCTGGTGTTCGGTACCCTGCACACCAGCTCGGCGGCCAAGACCATAGACCGTATC
ATCGACGTGTTCCCCGGGGCGGAGAAGGACATGGTGCGTTCCATGCTGTCCGAATCCCTGCGGGCGGTCATCTCCCAGAC
CCTGCTGAAGAAGACCGGCGGCGGCCGGGTGGCGGCCCACGAGATCATGATCGGCATCCCCGCCATTCGTAACCTGATCC
GGGAAGACAAGATTGCCCAGATGTACTCCGTCATCCAGACCGGGATGATGCACGGCATGCAAACCATGGATCAGTGCCTC
AAGCAGTTGGTGGCCCGGGGCGTCGTCAACGTCGCGGACGCCAAGGCCAAGGCCGTGGATCCCAACAGCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.779

100

0.788
  pilT Acinetobacter baumannii D1279779

78.779

100

0.788
  pilT Acinetobacter baumannii strain A118

78.779

100

0.788
  pilT Acinetobacter baylyi ADP1

78.779

100

0.788
  pilT Acinetobacter nosocomialis M2

78.488

100

0.785
  pilT Pseudomonas aeruginosa PAK

78.198

100

0.782
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

74.481

97.965

0.73
  pilT Vibrio cholerae strain A1552

74.481

97.965

0.73
  pilT Legionella pneumophila strain Lp02

73.235

98.837

0.724
  pilT Legionella pneumophila strain ERS1305867

73.235

98.837

0.724
  pilT Neisseria meningitidis 8013

68.406

100

0.686
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.683
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.047

99.419

0.517
  pilU Vibrio cholerae strain A1552

40.58

100

0.407
  pilU Acinetobacter baylyi ADP1

40.173

100

0.404
  pilU Pseudomonas stutzeri DSM 10701

40

100

0.401

Multiple sequence alignment