Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABJ384_RS11395 Genome accession   NZ_CP157981
Coordinates   2344115..2345152 (+) Length   345 a.a.
NCBI ID   WP_086196416.1    Uniprot ID   -
Organism   Acinetobacter sp. A1-4-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2339115..2350152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABJ384_RS11385 (ABJ384_11375) - 2342347..2343144 (-) 798 WP_349927422.1 thioesterase family protein -
  ABJ384_RS11390 (ABJ384_11380) - 2343305..2343991 (-) 687 WP_349927424.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABJ384_RS11395 (ABJ384_11385) pilT 2344115..2345152 (+) 1038 WP_086196416.1 type IV pilus twitching motility protein PilT Machinery gene
  ABJ384_RS11400 (ABJ384_11390) pilU 2345178..2346299 (+) 1122 WP_349927426.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABJ384_RS11405 (ABJ384_11395) fur 2346371..2346808 (-) 438 WP_086175193.1 ferric iron uptake transcriptional regulator -
  ABJ384_RS11410 (ABJ384_11400) bamE 2346921..2347319 (+) 399 WP_166171149.1 outer membrane protein assembly factor BamE -
  ABJ384_RS11415 (ABJ384_11405) - 2347365..2347688 (-) 324 WP_349927428.1 RnfH family protein -
  ABJ384_RS11420 (ABJ384_11410) - 2347688..2348770 (-) 1083 WP_349927429.1 hypothetical protein -
  ABJ384_RS11425 (ABJ384_11415) - 2348913..2349368 (-) 456 WP_349927430.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38241.92 Da        Isoelectric Point: 6.6655

>NTDB_id=1009480 ABJ384_RS11395 WP_086196416.1 2344115..2345152(+) (pilT) [Acinetobacter sp. A1-4-2]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDFEEKLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTIEDLGMGQIFKDICNYPRGIVLVTGPTGSGKSTTLAAMIDYINDQRYDHILTVED
PIEFVHESKKCLVNQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVAKGVISAQTARTMAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1009480 ABJ384_RS11395 WP_086196416.1 2344115..2345152(+) (pilT) [Acinetobacter sp. A1-4-2]
ATGGATATCACTGAACTTTTAGCGTTTTCAGCTAAAAACGGGGCATCAGATTTACACTTGTCTGCGGGGATGCCGCCACT
GATCCGTGTCGATGGTGAAGTTCGCCGTATTAATCTGCCTGCTTTAGAACATAAAGAAGTACACAAACTTGTGTATGACA
TTATGAATGATAAACAGCGTCGTGATTTTGAAGAAAAGCTAGAAACAGACTTTTCATTTGAAGTTCCAGGTGTAGCTCGT
TTCCGTGTGAATGCATTTAACCAGAACCGTGGTGCAGGTGCCGTGTTCCGTACCATTCCTTCAAAAGTACTTACCATTGA
AGATCTGGGTATGGGTCAGATCTTTAAAGATATCTGTAATTACCCGCGTGGTATCGTACTGGTGACCGGACCTACAGGTT
CAGGTAAATCTACTACACTAGCGGCCATGATCGACTATATTAATGATCAGCGTTATGACCATATCCTGACGGTAGAAGAC
CCGATCGAATTTGTACACGAATCTAAAAAATGCCTAGTCAACCAGCGTGAAGTACATCGCGATACCCATGGCTTTAATGA
AGCTCTTCGTTCAGCACTGCGTGAAGACCCGGATATTATTCTGGTCGGTGAGATGCGTGACCTTGAAACCATCCGTTTGG
CATTAACTGCCGCTGAAACCGGTCACCTGGTGTTTGGAACGCTACATACCACCTCTGCAGCAAAAACCATTGACCGTGTG
ATTGACGTATTCCCGGCAGAAGAAAAAGACATGGTTCGTGCCATGTTGTCTGAATCCTTACAAGCCGTGATTTCTCAAAC
ATTATTGAAGAAAAATGGTGGTGGTCGTGTTGCAGCACATGAAATCATGATTGGTATTCCTGCCATCCGTAACCTGATCC
GTGAAAACAAAGTGGCTCAAATGTATTCTGCCATTCAAACTGGTGCCAACTACGGTATGACCACGCTTGATCAAAGTTTA
AAAACCCTGGTTGCAAAAGGTGTGATCAGTGCGCAAACTGCCCGCACCATGGCAAAACAACCAGAATCATTCTTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii strain A118

94.493

100

0.945
  pilT Acinetobacter baumannii D1279779

94.493

100

0.945
  pilT Acinetobacter nosocomialis M2

94.203

100

0.942
  pilT Acinetobacter baylyi ADP1

87.209

99.71

0.87
  pilT Pseudomonas aeruginosa PAK

84.884

99.71

0.846
  pilT Pseudomonas stutzeri DSM 10701

84.593

99.71

0.843
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.643

97.391

0.678
  pilT Vibrio cholerae strain A1552

69.643

97.391

0.678
  pilT Neisseria meningitidis 8013

66.957

100

0.67
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.667
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.525
  pilU Vibrio cholerae strain A1552

42.388

97.101

0.412
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.101

0.397
  pilU Acinetobacter baylyi ADP1

39.306

100

0.394

Multiple sequence alignment