Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABJ384_RS11400 Genome accession   NZ_CP157981
Coordinates   2345178..2346299 (+) Length   373 a.a.
NCBI ID   WP_349927426.1    Uniprot ID   A0AAU7SVS4
Organism   Acinetobacter sp. A1-4-2     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2340178..2351299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABJ384_RS11385 (ABJ384_11375) - 2342347..2343144 (-) 798 WP_349927422.1 thioesterase family protein -
  ABJ384_RS11390 (ABJ384_11380) - 2343305..2343991 (-) 687 WP_349927424.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABJ384_RS11395 (ABJ384_11385) pilT 2344115..2345152 (+) 1038 WP_086196416.1 type IV pilus twitching motility protein PilT Machinery gene
  ABJ384_RS11400 (ABJ384_11390) pilU 2345178..2346299 (+) 1122 WP_349927426.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABJ384_RS11405 (ABJ384_11395) fur 2346371..2346808 (-) 438 WP_086175193.1 ferric iron uptake transcriptional regulator -
  ABJ384_RS11410 (ABJ384_11400) bamE 2346921..2347319 (+) 399 WP_166171149.1 outer membrane protein assembly factor BamE -
  ABJ384_RS11415 (ABJ384_11405) - 2347365..2347688 (-) 324 WP_349927428.1 RnfH family protein -
  ABJ384_RS11420 (ABJ384_11410) - 2347688..2348770 (-) 1083 WP_349927429.1 hypothetical protein -
  ABJ384_RS11425 (ABJ384_11415) - 2348913..2349368 (-) 456 WP_349927430.1 bacteriohemerythrin -
  ABJ384_RS11430 (ABJ384_11420) - 2349786..2350634 (-) 849 WP_349927431.1 class II glutamine amidotransferase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41922.25 Da        Isoelectric Point: 6.5950

>NTDB_id=1009481 ABJ384_RS11400 WP_349927426.1 2345178..2346299(+) (pilU) [Acinetobacter sp. A1-4-2]
MDFNDLLNLMIQQQASDLFITADVEPSMKINGQILPIAKTKLTGEIVGQLLNSIMSEKQRKEFAETRECNFAISNRDKTA
RFRVSAFQQRDEPGMVLRRIETVIPTMEQLKLPPILKELAMTKRGIIMFVGATGTGKSTSLASIIGYRNQNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQALDR
IIHFFEADRHNQLFMDLSLNMKAIVAQQLIPTLDGTGRRAAIEILINSPLISDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALFDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGDRLMRASSNITFDGQA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1009481 ABJ384_RS11400 WP_349927426.1 2345178..2346299(+) (pilU) [Acinetobacter sp. A1-4-2]
ATGGATTTTAACGACTTACTGAATTTGATGATTCAACAACAGGCATCCGACTTATTTATTACTGCCGATGTTGAACCGTC
CATGAAAATTAATGGGCAGATTCTGCCCATTGCAAAGACCAAGCTGACTGGCGAAATTGTTGGACAACTTTTAAACTCAA
TAATGAGTGAAAAGCAACGTAAAGAATTTGCTGAAACACGCGAATGTAACTTTGCCATCAGCAACCGCGACAAAACTGCC
CGTTTCCGTGTCAGTGCATTTCAACAACGCGATGAACCCGGTATGGTATTGCGTCGTATCGAAACCGTCATTCCGACCAT
GGAACAGCTGAAGCTACCGCCTATTTTAAAAGAATTGGCGATGACCAAGCGCGGTATCATCATGTTTGTGGGTGCAACCG
GTACAGGTAAATCGACCTCTTTGGCTTCCATTATTGGTTATCGTAACCAGAATTCCAAAGGTCATATCATTACCATTGAA
GACCCGATTGAATTTATTCATGAACATGCGGGCTGTATCATTACCCAGCGTGAAGTGGGCATTGATACCGACTCTTTTGA
AGTTGCTTTGAAAAATACCCTGCGTCAGGCACCTGATGTTATTTTGATTGGTGAGATTCGTTCGCGCGAAACCATGGATT
ATGCGATTGCCTTTGCGGAAACCGGTCACTTGGTATTTGCCACGCTGCATGCCAACAACGCCAACCAGGCACTTGACCGG
ATTATTCACTTCTTTGAAGCAGACCGGCATAACCAGTTGTTTATGGATTTGTCTTTAAACATGAAAGCGATTGTGGCCCA
GCAACTGATTCCAACGCTGGATGGCACTGGACGCCGTGCTGCAATTGAAATCCTGATCAATTCACCTTTAATTTCAGACC
TAATCCGTAAAGGTGATGTGCATGAAATTAAAGACCTGATGAAACGTTCACGTGAACTGGGCATGCAAACCTTTGACCAG
GCCCTGTTTGACTTGTACAAAGCCAAGCAAATTAGCTATAAAGATGCATTGAAACATGCCGACTCACCAAACGATCTGCG
TTTGCAAATCAAGTTGTCGGAAGAAGGCGGCGATCGCTTAATGCGCGCCAGTTCGAATATTACTTTCGATGGTCAGGCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

75.397

100

0.764
  pilU Pseudomonas stutzeri DSM 10701

70.081

99.464

0.697
  pilU Vibrio cholerae strain A1552

54.595

99.196

0.542
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.197

92.761

0.391
  pilT Legionella pneumophila strain Lp02

41.716

90.617

0.378
  pilT Legionella pneumophila strain ERS1305867

41.716

90.617

0.378
  pilT Acinetobacter baumannii D1279779

40.233

91.957

0.37
  pilT Acinetobacter nosocomialis M2

40.233

91.957

0.37
  pilT Acinetobacter baumannii strain A118

40.233

91.957

0.37
  pilT Pseudomonas aeruginosa PAK

39.884

92.761

0.37
  pilT Acinetobacter baylyi ADP1

39.65

91.957

0.365
  pilT Pseudomonas stutzeri DSM 10701

39.017

92.761

0.362

Multiple sequence alignment