Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABNK63_RS04105 Genome accession   NZ_CP157948
Coordinates   941313..942350 (+) Length   345 a.a.
NCBI ID   WP_007809667.1    Uniprot ID   I4VUT4
Organism   Rhodanobacter sp. IGA1.0     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 936313..947350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNK63_RS04080 (ABNK63_04075) pdxH 936805..937392 (+) 588 WP_040673604.1 pyridoxamine 5'-phosphate oxidase -
  ABNK63_RS04085 (ABNK63_04080) - 937389..937829 (+) 441 WP_007810023.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  ABNK63_RS04090 (ABNK63_04085) - 937834..938643 (+) 810 WP_350016767.1 cobalamin-binding protein -
  ABNK63_RS04095 (ABNK63_04090) - 939535..940110 (-) 576 WP_350016768.1 YggT family protein -
  ABNK63_RS04100 (ABNK63_04095) proC 940107..940931 (-) 825 WP_350016769.1 pyrroline-5-carboxylate reductase -
  ABNK63_RS04105 (ABNK63_04100) pilT 941313..942350 (+) 1038 WP_007809667.1 type IV pilus twitching motility protein PilT Machinery gene
  ABNK63_RS04110 (ABNK63_04105) pilU 942504..943673 (+) 1170 WP_204110692.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABNK63_RS04115 (ABNK63_04110) - 943757..944125 (-) 369 WP_007809653.1 BON domain-containing protein -
  ABNK63_RS04120 (ABNK63_04115) - 944361..946142 (-) 1782 WP_350016770.1 hypothetical protein -
  ABNK63_RS04125 (ABNK63_04120) - 946227..947153 (-) 927 WP_007809648.1 aspartate carbamoyltransferase catalytic subunit -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38531.17 Da        Isoelectric Point: 6.7246

>NTDB_id=1009306 ABNK63_RS04105 WP_007809667.1 941313..942350(+) (pilT) [Rhodanobacter sp. IGA1.0]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALEHKQVHSLIYDIMSDKQRRDYEEFYETDFSFEIPGLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPRVFKELIEQPQGLILVTGPTGSGKSTTLAAMVDHINKNEYGHMLSVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFATVHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQSLLKKVGGGRVAAHEIMVGIPAIRNLIREDKVAQMYSSIQTGQQFGMQTLDQCL
QDLVKRGLVTRQEASMYAKNKDSFK

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1009306 ABNK63_RS04105 WP_007809667.1 941313..942350(+) (pilT) [Rhodanobacter sp. IGA1.0]
ATGGACATTGCCGAACTGCTTGCCTTCTCGGTAAAGAACAAGGCCTCGGACTTGCATCTGTCCGCCGGCCTGCCACCGAT
GATCCGCGTTGATGGCGATGTCCGTCGCATCAACATCCCTGCGCTGGAACACAAGCAGGTGCACTCGCTGATCTACGACA
TCATGTCGGACAAGCAGCGGCGCGACTACGAGGAGTTCTACGAGACCGACTTCTCGTTCGAGATTCCCGGGCTGGCCCGT
TTCCGCGTCAACGCGTTCAACCAGAACCGGGGCGCCGGCGCGGTGTTCCGCACCATTCCGTCCGAAGTGCTGACCCTGGA
AGACCTGGGTTGCCCCCGGGTGTTCAAGGAGCTGATCGAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGGTCGACCACATCAACAAGAACGAATACGGCCACATGCTCTCGGTCGAGGAC
CCGATCGAGTTCGTGCATACCTCGCAAAAGTGCCTGATCAACCAGCGCGAGGTGCATCGCGACACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAGGACCCGGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAGACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGCCACCGTGCACACCAGCTCGGCGGCGAAGACCATCGACCGCATC
ATCGATGTGTTCCCTGCCGGCGAGAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGCGCGGTCATCTCGCAGTC
GCTGCTGAAGAAGGTCGGTGGCGGCCGCGTCGCGGCGCACGAGATCATGGTCGGCATCCCGGCGATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTATTCGTCGATCCAGACCGGCCAGCAGTTCGGCATGCAGACGCTGGACCAGTGCCTG
CAGGACCTGGTCAAGCGTGGCCTGGTGACCCGTCAGGAGGCCTCGATGTACGCCAAGAACAAGGACAGCTTCAAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I4VUT4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

77.616

99.71

0.774
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.27

96.522

0.678
  pilT Vibrio cholerae strain A1552

70.27

96.522

0.678
  pilT Neisseria meningitidis 8013

63.768

100

0.638
  pilT Neisseria gonorrhoeae MS11

63.478

100

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.547

95.942

0.475
  pilU Pseudomonas stutzeri DSM 10701

39.884

100

0.4
  pilU Vibrio cholerae strain A1552

39.017

100

0.391
  pilU Acinetobacter baylyi ADP1

38.329

100

0.386

Multiple sequence alignment