Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABNP33_RS14135 Genome accession   NZ_CP157872
Coordinates   2997689..2998726 (+) Length   345 a.a.
NCBI ID   WP_057086001.1    Uniprot ID   -
Organism   Acinetobacter pittii strain bac73     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2992689..3003726
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNP33_RS14125 (ABNP33_14120) sbcC 2993073..2996669 (+) 3597 WP_349913324.1 AAA family ATPase -
  ABNP33_RS14130 (ABNP33_14125) yggS 2996870..2997562 (-) 693 WP_349913327.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABNP33_RS14135 (ABNP33_14130) pilT 2997689..2998726 (+) 1038 WP_057086001.1 type IV pilus twitching motility protein PilT Machinery gene
  ABNP33_RS14140 (ABNP33_14135) pilU 2998746..2999864 (+) 1119 WP_060465360.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABNP33_RS14145 (ABNP33_14140) fur 2999934..3000371 (-) 438 WP_002121758.1 ferric iron uptake transcriptional regulator -
  ABNP33_RS14150 (ABNP33_14145) omlA 3000483..3000881 (+) 399 WP_002121740.1 outer membrane protein assembly factor BamE -
  ABNP33_RS14155 (ABNP33_14150) - 3000946..3001260 (-) 315 WP_017481335.1 RnfH family protein -
  ABNP33_RS14160 (ABNP33_14155) - 3001260..3002327 (-) 1068 WP_130118783.1 hypothetical protein -
  ABNP33_RS14165 (ABNP33_14160) - 3002481..3002936 (-) 456 WP_002121698.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38404.00 Da        Isoelectric Point: 6.7269

>NTDB_id=1008699 ABNP33_RS14135 WP_057086001.1 2997689..2998726(+) (pilT) [Acinetobacter pittii strain bac73]
MDITELLAFSVKNGASDLHLSAGMPPMIRVDGEVRRINLPALDHKDVHRLVYDIMNDKQRRDYEEKLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGQIFKDICDYPRGIVLVTGPTGSGKSTTLAAMMDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANHGMTTLDQSL
KNLVSRGVISPQTARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1008699 ABNP33_RS14135 WP_057086001.1 2997689..2998726(+) (pilT) [Acinetobacter pittii strain bac73]
ATGGATATTACAGAGCTACTCGCCTTCTCTGTGAAAAATGGCGCGTCCGATTTACATTTGTCTGCTGGCATGCCGCCAAT
GATTCGCGTTGATGGTGAAGTCCGTCGCATTAACTTACCTGCTCTAGATCATAAAGATGTTCATCGTTTGGTCTACGACA
TCATGAATGATAAACAGCGTCGTGACTACGAAGAAAAACTCGAAACCGACTTTTCTTTTGAAGTACCCAACATTGCTCGT
TTCCGTGTTAACGCATTTAATCAAAACCGTGGTGCGGGTGCAGTGTTTCGTACGATTCCGTCCAAAGTACTCACCATGGA
AGATTTAGGCTTAGGTCAAATCTTTAAAGATATTTGTGATTATCCACGCGGCATTGTACTGGTGACAGGTCCAACAGGTT
CTGGTAAATCGACGACACTTGCAGCGATGATGGACTACATCAACGAAAACCGTTATGACCACATTCTAACGGTTGAAGAC
CCTATCGAATTTGTACACCAATCTAAGAAATGTCTCATCAACCAACGTGAAGTGCATCGTGATACACACGGCTTTAATGA
GGCACTACGTTCAGCACTGCGTGAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCTTGAGACTATTCGTTTAG
CACTTACTGCTGCCGAAACAGGTCATTTAGTATTTGGTACTCTTCATACCACTTCTGCTGCGAAAACCATTGACCGTGTA
ATTGACGTATTCCCAGCCGAAGAAAAAGACATGGTTCGTGCCATGCTCTCTGAATCTTTACAGGCGGTTATTTCACAAAC
CCTGCTTAAAAAGAATGGCGGCGGCCGTGTGGCAGCTCATGAAATCATGATTGGTATTCCTGCCATTCGTAACTTAATTC
GTGAAAATAAAGTCGCGCAAATGTACTCAGCTATTCAGACTGGCGCAAATCATGGCATGACCACCCTCGACCAAAGCTTA
AAGAATTTGGTGTCTCGCGGTGTGATCAGCCCGCAAACGGCTCGTACTGCTGCTAAACAGCCTGAATCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

98.841

100

0.988
  pilT Acinetobacter baumannii D1279779

98.551

100

0.986
  pilT Acinetobacter baumannii strain A118

98.551

100

0.986
  pilT Acinetobacter baylyi ADP1

88.663

99.71

0.884
  pilT Pseudomonas stutzeri DSM 10701

83.14

99.71

0.829
  pilT Pseudomonas aeruginosa PAK

82.849

99.71

0.826
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.131

97.391

0.693
  pilT Vibrio cholerae strain A1552

71.131

97.391

0.693
  pilT Neisseria meningitidis 8013

67.826

100

0.678
  pilT Neisseria gonorrhoeae MS11

67.536

100

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.519
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.194

97.101

0.4
  pilU Acinetobacter baylyi ADP1

39.017

100

0.391

Multiple sequence alignment