Detailed information NTG1008700

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABNP33_RS14140
• Genome accession: NZ_CP157872
• Coordinates: 2998746..2999864 (+)
• Length: 372 a.a.
• NCBI ID: WP_060465360.1
• Uniprot ID: A0A2I6LWN2
• Organism: Acinetobacter pittii strain bac73
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2993746..3004864

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABNP33_RS14130 (ABNP33_14125)	yggS	2996870..2997562 (-)	693	WP_349913327.1	YggS family pyridoxal phosphate-dependent enzyme	-
ABNP33_RS14135 (ABNP33_14130)	pilT	2997689..2998726 (+)	1038	WP_057086001.1	type IV pilus twitching motility protein PilT	Machinery gene
ABNP33_RS14140 (ABNP33_14135)	pilU	2998746..2999864 (+)	1119	WP_060465360.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ABNP33_RS14145 (ABNP33_14140)	fur	2999934..3000371 (-)	438	WP_002121758.1	ferric iron uptake transcriptional regulator	-
ABNP33_RS14150 (ABNP33_14145)	omlA	3000483..3000881 (+)	399	WP_002121740.1	outer membrane protein assembly factor BamE	-
ABNP33_RS14155 (ABNP33_14150)	-	3000946..3001260 (-)	315	WP_017481335.1	RnfH family protein	-
ABNP33_RS14160 (ABNP33_14155)	-	3001260..3002327 (-)	1068	WP_130118783.1	hypothetical protein	-
ABNP33_RS14165 (ABNP33_14160)	-	3002481..3002936 (-)	456	WP_002121698.1	bacteriohemerythrin	-
ABNP33_RS14170 (ABNP33_14165)	-	3003188..3004036 (-)	849	WP_032004227.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41917.12 Da
• Isoelectric Point: 6.6403

>NTDB_id=1008700 ABNP33_RS14140 WP_060465360.1 2998746..2999864(+) (pilU) [Acinetobacter pittii strain bac73]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEIIGQLLHSIMSEKQRKEFAETRECNFAIMNRDKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISYRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKSGQITYKDALKHADSPNDLRLTIKLAEEGPDQIAGTNQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=1008700 ABNP33_RS14140 WP_060465360.1 2998746..2999864(+) (pilU) [Acinetobacter pittii strain bac73]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCACCATC
GATGAAGATTAACGGGCAAATTGTTCCCATTTCAAAAAACAGTCTTTCAGGCGAAATTATTGGGCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCTGAGACTCGTGAATGTAACTTTGCCATTATGAACCGTGATAAAACTGCC
CGCTTTCGTGTGAGTGCTTTTCAGCAACGCGACATGCCAGGCATGGTGCTACGTCGAATTGAAACCAAAATTCCTTCAAT
TGACGACTTACAATTACCGCCAGTGCTTAAAGATTTATCGATGACCAAACGCGGCATCATTATTTTTGTAGGGGCAACAG
GTACAGGTAAATCGACATCATTGGCTTCAATGATTAGCTATCGTAACCATAACTCTAAAGGTCACATCATTACCATTGAA
GACCCTATCGAGTTTATTCATGAACATGCTGGTTGCATCATTACCCAACGTGAAGTCGGGATTGATACAGACTCATTCGA
AATTGCCTTAAAGAATACTTTGCGACAAGCACCCGATGTAATCTTGATTGGTGAGATTCGCTCTCGTGAAGTCATGGACT
ATGCGATTGGTTTTGCCGAAACAGGTCACCTTGTATTAGCCACAATGCATGCGAACAACGCCAACCAAGCACTTGACCGT
ATTATTCACTTCTTTGAAAGTGACCGTCATAGCCAGCTCTACATGGATTTATCGCTTAATTTAAAGGCCATGATTGCACA
GCAACTTATTCCGACTCCAGATGGTAATTCTCGCCGTGCAGCGATTGAGATTCTCATTAACTCACCATTAATTTCAGACT
ATATTCGTAAAGGCGAAATTCATGAAATTAAAGATTTAATGAAACGCTCACGTGAACTCGGTATGCAAACTTTTGACCAA
GCACTGTTTGATCTTTATAAGTCGGGCCAAATTACCTACAAAGATGCATTAAAACATGCCGACTCACCAAACGATTTGCG
TTTAACAATTAAGCTTGCCGAAGAAGGTCCTGATCAAATTGCAGGCACAAATCAGCATTTAACCTTCGACAGACAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (33-270)

3D structure

• AlphaFold DB: A0A2I6LWN2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.397	100	0.766
pilU	Pseudomonas stutzeri DSM 10701	68.464	99.731	0.683
pilU	Vibrio cholerae strain A1552	54.054	99.462	0.538
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.934	97.849	0.401
pilT	Legionella pneumophila strain Lp02	39.941	90.86	0.363
pilT	Legionella pneumophila strain ERS1305867	39.941	90.86	0.363