Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QUE03_RS13475 Genome accession   NZ_AP027364
Coordinates   2931556..2932599 (-) Length   347 a.a.
NCBI ID   WP_286262301.1    Uniprot ID   -
Organism   Thalassotalea atypica strain JCM 31894     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2926556..2937599
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE03_RS13450 - 2927430..2927984 (+) 555 WP_286262291.1 YqgE/AlgH family protein -
  QUE03_RS13455 ruvX 2927984..2928430 (+) 447 WP_286262293.1 Holliday junction resolvase RuvX -
  QUE03_RS13460 - 2928527..2929450 (+) 924 WP_286262295.1 AEC family transporter -
  QUE03_RS13465 - 2929599..2930318 (+) 720 WP_286262297.1 transporter substrate-binding domain-containing protein -
  QUE03_RS13470 pilU 2930392..2931531 (-) 1140 WP_286262299.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUE03_RS13475 pilT 2931556..2932599 (-) 1044 WP_286262301.1 type IV pilus twitching motility protein PilT Machinery gene
  QUE03_RS13480 - 2932632..2933306 (+) 675 WP_286262303.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUE03_RS13485 proC 2933333..2934145 (+) 813 WP_286262305.1 pyrroline-5-carboxylate reductase -
  QUE03_RS13490 - 2934239..2934787 (+) 549 WP_286262307.1 YggT family protein -
  QUE03_RS13495 - 2934851..2935300 (+) 450 WP_286262309.1 DUF4426 domain-containing protein -
  QUE03_RS13500 - 2935431..2936342 (-) 912 WP_286262311.1 EamA family transporter -
  QUE03_RS13505 - 2936543..2937127 (+) 585 WP_286262313.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38558.96 Da        Isoelectric Point: 5.6230

>NTDB_id=100860 QUE03_RS13475 WP_286262301.1 2931556..2932599(-) (pilT) [Thalassotalea atypica strain JCM 31894]
MDITELLAFSVDHNASDLHLSTGTPPSIRVDGDVRKLNIPAFDAKDVNALVYDIMNDRQRKEYEENLEVDFSFEVPNLAR
FRVNAFNQNRGPAAVFRTIPSKVLTLDDLGCPDIFRDISDTPRGLVLVTGPTGSGKSTTLAAMVDYINSSKNDHILTIED
PIEFVHDNKMCLINQREVHRDTLSFSNALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPAEEKSMVRSMLSESLRAVISQTLVKKVGGGRVAAHEIMIGVPAIRNLIREDKIAQMYSAIQTGMSHGMQTMDQCL
QNLVNRGLITRQDAMSKAQDKNQFSSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=100860 QUE03_RS13475 WP_286262301.1 2931556..2932599(-) (pilT) [Thalassotalea atypica strain JCM 31894]
ATGGATATTACCGAATTATTAGCTTTTAGTGTTGATCATAATGCATCTGATTTGCATTTATCGACAGGCACACCGCCGTC
AATTCGTGTTGATGGTGATGTGCGTAAACTCAATATTCCAGCCTTTGATGCAAAAGACGTAAATGCTTTGGTCTATGATA
TTATGAATGACCGCCAACGCAAAGAATATGAAGAAAATTTAGAGGTCGATTTCTCGTTTGAAGTACCAAATTTAGCGCGA
TTCAGGGTCAATGCTTTTAATCAAAATCGTGGTCCTGCTGCTGTATTTCGTACCATCCCATCAAAGGTGCTCACTTTAGA
TGATTTAGGTTGTCCTGATATTTTCCGTGATATTTCTGACACCCCACGTGGTTTAGTGTTAGTAACAGGGCCTACAGGCT
CAGGTAAGTCGACTACATTGGCCGCCATGGTTGATTACATTAACAGCAGTAAAAATGATCATATTCTAACCATTGAAGAT
CCGATTGAATTTGTTCACGACAACAAGATGTGTTTGATTAACCAACGTGAAGTTCACCGTGATACGTTAAGTTTTAGTAA
CGCACTTCGTTCAGCCTTACGTGAAGATCCCGATGTTATTCTTGTTGGTGAAATGCGTGACTTGGAAACCATTCGCCTTG
CTATGACTGCCGCGGAAACAGGTCATTTAGTGTTTGGTACCTTGCATACCACGTCTGCACCTAAAACCATTGACCGTATT
ATTGATGTTTTTCCCGCTGAGGAAAAATCCATGGTTCGCTCAATGTTATCAGAATCTCTGCGTGCCGTTATTTCACAAAC
CTTAGTGAAGAAGGTCGGTGGTGGTCGCGTAGCAGCACATGAAATAATGATAGGCGTACCCGCCATTCGTAACTTGATTC
GTGAAGATAAAATAGCCCAAATGTACTCCGCCATTCAAACAGGGATGTCACACGGTATGCAAACCATGGATCAATGTTTA
CAAAACCTAGTCAACCGCGGCCTAATTACCCGCCAAGACGCGATGTCAAAAGCGCAAGACAAAAATCAGTTTTCTAGCTT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.135

0.778
  pilT Acinetobacter nosocomialis M2

77.616

99.135

0.769
  pilT Acinetobacter baumannii D1279779

77.616

99.135

0.769
  pilT Acinetobacter baumannii strain A118

77.616

99.135

0.769
  pilT Pseudomonas aeruginosa PAK

77.035

99.135

0.764
  pilT Acinetobacter baylyi ADP1

76.744

99.135

0.761
  pilT Legionella pneumophila strain Lp02

72.965

99.135

0.723
  pilT Legionella pneumophila strain ERS1305867

72.965

99.135

0.723
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

72.271

97.695

0.706
  pilT Vibrio cholerae strain A1552

72.271

97.695

0.706
  pilT Neisseria meningitidis 8013

68.497

99.712

0.683
  pilT Neisseria gonorrhoeae MS11

68.208

99.712

0.68
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.757

99.712

0.536
  pilU Pseudomonas stutzeri DSM 10701

41.691

98.847

0.412
  pilU Vibrio cholerae strain A1552

41.691

98.847

0.412
  pilU Acinetobacter baylyi ADP1

39.826

99.135

0.395

Multiple sequence alignment