Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QUE03_RS13470 Genome accession   NZ_AP027364
Coordinates   2930392..2931531 (-) Length   379 a.a.
NCBI ID   WP_286262299.1    Uniprot ID   -
Organism   Thalassotalea atypica strain JCM 31894     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2925392..2936531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUE03_RS13440 rsmE 2925693..2926421 (+) 729 WP_286262285.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  QUE03_RS13445 gshB 2926431..2927384 (+) 954 WP_286262288.1 glutathione synthase -
  QUE03_RS13450 - 2927430..2927984 (+) 555 WP_286262291.1 YqgE/AlgH family protein -
  QUE03_RS13455 ruvX 2927984..2928430 (+) 447 WP_286262293.1 Holliday junction resolvase RuvX -
  QUE03_RS13460 - 2928527..2929450 (+) 924 WP_286262295.1 AEC family transporter -
  QUE03_RS13465 - 2929599..2930318 (+) 720 WP_286262297.1 transporter substrate-binding domain-containing protein -
  QUE03_RS13470 pilU 2930392..2931531 (-) 1140 WP_286262299.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUE03_RS13475 pilT 2931556..2932599 (-) 1044 WP_286262301.1 type IV pilus twitching motility protein PilT Machinery gene
  QUE03_RS13480 - 2932632..2933306 (+) 675 WP_286262303.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUE03_RS13485 proC 2933333..2934145 (+) 813 WP_286262305.1 pyrroline-5-carboxylate reductase -
  QUE03_RS13490 - 2934239..2934787 (+) 549 WP_286262307.1 YggT family protein -
  QUE03_RS13495 - 2934851..2935300 (+) 450 WP_286262309.1 DUF4426 domain-containing protein -
  QUE03_RS13500 - 2935431..2936342 (-) 912 WP_286262311.1 EamA family transporter -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 41872.80 Da        Isoelectric Point: 5.1599

>NTDB_id=100859 QUE03_RS13470 WP_286262299.1 2930392..2931531(-) (pilU) [Thalassotalea atypica strain JCM 31894]
MSFHQLLQKMVDADASDMFVTAKLPVSAKINGELHPIDNDILSAEASLSLVHDAMNEKQKNQFDDEKECNFAISIDDIGR
FRVSAFWQRDMAGMVIRRIVTEIPEADDLGLPPILKDVVMSKRGLVLFVGGTGTGKSTSMAALIGYRNKNARGHILTIED
PVEFVHEHGKSMITQREVGLDTESFDAALKSSLRQAPDVILIGEIRSQEIMEHALSFAETGHLCIATLHANNANQAIDRI
MHLVPAEQHQKLQFDLALNLRGIIAQQLVPTKDGHGRVAAIEILLNSPYISELIKKGDVGSIKEVMEKSREQGMQTFDRA
LFDLYEQGLINYADALHHADSPNDLRLMIKLRSNDQGGVGGLQGVTLAGLEDDKDKDMF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=100859 QUE03_RS13470 WP_286262299.1 2930392..2931531(-) (pilU) [Thalassotalea atypica strain JCM 31894]
ATGAGTTTTCATCAGCTATTACAAAAAATGGTGGATGCCGATGCGTCAGATATGTTTGTTACGGCGAAATTACCCGTTAG
TGCGAAAATAAACGGCGAGCTGCACCCTATAGATAATGATATTTTATCTGCTGAAGCATCTTTGTCACTAGTGCACGACG
CGATGAATGAGAAGCAGAAAAATCAATTTGACGATGAAAAAGAATGCAACTTTGCTATTTCTATTGATGATATTGGACGA
TTTCGCGTTTCAGCCTTTTGGCAACGAGACATGGCCGGTATGGTTATCCGCCGTATCGTTACCGAAATACCTGAAGCAGA
TGATTTAGGTTTACCGCCGATACTAAAAGATGTCGTGATGTCTAAACGTGGTTTAGTGTTATTCGTTGGCGGTACCGGTA
CGGGTAAATCAACATCAATGGCCGCATTAATCGGCTATCGCAATAAGAATGCACGTGGTCACATTCTAACGATTGAAGAC
CCGGTAGAATTTGTCCATGAACACGGTAAATCCATGATCACCCAACGTGAAGTTGGTCTGGATACAGAAAGCTTTGACGC
GGCATTGAAAAGCTCATTGCGCCAAGCACCCGATGTCATTTTAATCGGTGAGATCCGTTCACAAGAGATTATGGAACATG
CCTTAAGTTTTGCTGAAACGGGGCATTTATGTATTGCCACCTTGCACGCGAACAACGCAAACCAAGCCATTGACCGTATT
ATGCATTTAGTGCCTGCGGAGCAGCATCAAAAACTGCAATTTGATTTAGCCTTAAACTTACGCGGCATTATTGCACAGCA
GCTGGTGCCAACTAAAGATGGTCACGGACGTGTCGCCGCCATCGAGATATTGTTGAACTCTCCTTATATTAGCGAATTGA
TTAAAAAAGGCGATGTTGGCAGTATCAAAGAAGTGATGGAAAAGTCTAGAGAGCAGGGGATGCAGACCTTTGACAGAGCG
TTATTCGATTTATATGAACAAGGACTGATTAACTATGCTGATGCACTTCATCACGCAGATTCACCAAACGATTTACGTTT
AATGATTAAGTTGCGATCTAACGACCAAGGTGGTGTCGGTGGATTACAAGGCGTGACTTTAGCTGGTCTCGAAGATGACA
AAGACAAAGACATGTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.201

99.736

0.58
  pilU Acinetobacter baylyi ADP1

58.523

92.876

0.544
  pilU Vibrio cholerae strain A1552

54.496

96.834

0.528
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.697

93.931

0.401
  pilT Acinetobacter nosocomialis M2

40.351

90.237

0.364
  pilT Acinetobacter baylyi ADP1

40.351

90.237

0.364
  pilT Acinetobacter baumannii D1279779

40.351

90.237

0.364
  pilT Acinetobacter baumannii strain A118

40.351

90.237

0.364
  pilT Pseudomonas aeruginosa PAK

39.71

91.029

0.361

Multiple sequence alignment