Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Q9L42_RS06100 Genome accession   NZ_CP157743
Coordinates   1246854..1247891 (-) Length   345 a.a.
NCBI ID   WP_305909309.1    Uniprot ID   A0AAU7NXL4
Organism   Methylomarinum sp. Ch1-1     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1241854..1252891
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9L42_RS06080 (Q9L42_006080) radC 1243146..1243820 (+) 675 WP_305909312.1 DNA repair protein RadC -
  Q9L42_RS06085 (Q9L42_006085) - 1243849..1244745 (-) 897 WP_305909311.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  Q9L42_RS06090 (Q9L42_006090) djlA 1244764..1245675 (+) 912 WP_349432296.1 co-chaperone DjlA -
  Q9L42_RS06095 (Q9L42_006095) pilU 1245696..1246835 (-) 1140 WP_349432298.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q9L42_RS06100 (Q9L42_006100) pilT 1246854..1247891 (-) 1038 WP_305909309.1 type IV pilus twitching motility protein PilT Machinery gene
  Q9L42_RS06105 (Q9L42_006105) - 1248104..1248796 (+) 693 WP_305909308.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q9L42_RS06110 (Q9L42_006110) - 1248711..1249592 (-) 882 WP_349432301.1 sulfite exporter TauE/SafE family protein -
  Q9L42_RS06115 (Q9L42_006115) cysB 1249690..1250679 (+) 990 WP_349432303.1 HTH-type transcriptional regulator CysB -
  Q9L42_RS06120 (Q9L42_006120) - 1251502..1251942 (+) 441 WP_305909305.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38353.28 Da        Isoelectric Point: 7.0363

>NTDB_id=1008100 Q9L42_RS06100 WP_305909309.1 1246854..1247891(-) (pilT) [Methylomarinum sp. Ch1-1]
MDIAELLAFSVKNNASDLHLSAGLPPMIRVDGDIRRINIPALDHKEVHALIYDIMNDKQRRDYEEFLETDFSFALPNVAR
FRVNAFNQERGAGAVFRTIPSEVLSLEQLNAPKFFAEVCKKPRGLILVTGPTGSGKSTTLAAMINHINSTEYAHILTVED
PIEFVHDSQKSLINQREVHRDTLGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
IDVFPAAEKDMIRSMLSESLQAVISQTLLKKIGGGRIAAHEIMVGTPAIRNLIREAKVAQMYSAIQTGRKEGMQTLDQNL
KELVQKGMINAKIAMVKAVNKDMFR

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1008100 Q9L42_RS06100 WP_305909309.1 1246854..1247891(-) (pilT) [Methylomarinum sp. Ch1-1]
ATGGACATCGCTGAATTATTGGCTTTTTCGGTAAAAAACAACGCATCAGATTTACACCTTTCAGCCGGCCTGCCGCCGAT
GATCAGAGTGGATGGCGACATACGCCGCATCAATATCCCCGCCCTCGATCACAAGGAAGTCCATGCGTTGATTTACGACA
TCATGAACGACAAACAACGCCGCGATTATGAAGAATTTCTGGAAACGGATTTTTCCTTCGCGTTGCCGAATGTCGCCCGT
TTCCGGGTCAATGCCTTCAATCAGGAACGGGGAGCTGGGGCGGTTTTCCGCACCATTCCCTCGGAAGTCTTATCGCTGGA
GCAACTCAATGCGCCGAAGTTCTTTGCCGAAGTCTGTAAGAAACCGAGAGGGTTGATCCTGGTGACCGGCCCAACCGGTT
CCGGTAAATCGACGACGCTGGCGGCGATGATCAATCACATCAACTCCACCGAATACGCCCACATTCTGACCGTCGAAGAC
CCGATCGAATTTGTCCACGACAGTCAGAAATCGCTGATCAACCAACGCGAAGTCCATCGCGATACGCTGGGATTCAATGA
AGCCTTACGTTCGGCGCTACGGGAAGACCCCGACATCATCTTGGTCGGCGAGATGCGGGACCTGGAAACGATACGTTTGG
CGTTGACCGCGGCGGAAACCGGCCACCTGGTTTTCGGCACCCTGCATACCACCTCGGCCGCGAAAACCATCGACCGGATC
ATCGACGTGTTCCCGGCGGCGGAGAAAGACATGATCCGCTCGATGCTGTCGGAATCGCTGCAAGCCGTGATCTCTCAGAC
CCTGCTGAAAAAAATCGGCGGCGGCCGGATCGCTGCCCATGAAATCATGGTGGGTACGCCGGCGATACGCAACTTAATCC
GGGAGGCAAAGGTCGCGCAAATGTACTCCGCGATTCAAACCGGTCGTAAAGAGGGCATGCAGACGTTGGATCAAAACCTG
AAGGAGTTGGTCCAAAAGGGCATGATCAATGCCAAAATCGCGATGGTCAAAGCCGTCAACAAAGACATGTTCCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

79.942

99.71

0.797
  pilT Acinetobacter nosocomialis M2

79.36

99.71

0.791
  pilT Acinetobacter baumannii D1279779

79.36

99.71

0.791
  pilT Acinetobacter baumannii strain A118

79.36

99.71

0.791
  pilT Pseudomonas aeruginosa PAK

80.178

97.971

0.786
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Legionella pneumophila strain Lp02

74.128

99.71

0.739
  pilT Legionella pneumophila strain ERS1305867

74.128

99.71

0.739
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.783

96.232

0.681
  pilT Vibrio cholerae strain A1552

70.783

96.232

0.681
  pilT Neisseria meningitidis 8013

67.638

99.42

0.672
  pilT Neisseria gonorrhoeae MS11

67.347

99.42

0.67
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Acinetobacter baylyi ADP1

40.286

100

0.409
  pilU Pseudomonas stutzeri DSM 10701

38.439

100

0.386
  pilU Vibrio cholerae strain A1552

38.439

100

0.386

Multiple sequence alignment