Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   Q9L42_RS06095 Genome accession   NZ_CP157743
Coordinates   1245696..1246835 (-) Length   379 a.a.
NCBI ID   WP_349432298.1    Uniprot ID   -
Organism   Methylomarinum sp. Ch1-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1240696..1251835
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q9L42_RS06070 (Q9L42_006070) dut 1241413..1241868 (-) 456 WP_349432294.1 dUTP diphosphatase -
  Q9L42_RS06075 (Q9L42_006075) coaBC 1241849..1243063 (-) 1215 WP_349432741.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  Q9L42_RS06080 (Q9L42_006080) radC 1243146..1243820 (+) 675 WP_305909312.1 DNA repair protein RadC -
  Q9L42_RS06085 (Q9L42_006085) - 1243849..1244745 (-) 897 WP_305909311.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
  Q9L42_RS06090 (Q9L42_006090) djlA 1244764..1245675 (+) 912 WP_349432296.1 co-chaperone DjlA -
  Q9L42_RS06095 (Q9L42_006095) pilU 1245696..1246835 (-) 1140 WP_349432298.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  Q9L42_RS06100 (Q9L42_006100) pilT 1246854..1247891 (-) 1038 WP_305909309.1 type IV pilus twitching motility protein PilT Machinery gene
  Q9L42_RS06105 (Q9L42_006105) - 1248104..1248796 (+) 693 WP_305909308.1 YggS family pyridoxal phosphate-dependent enzyme -
  Q9L42_RS06110 (Q9L42_006110) - 1248711..1249592 (-) 882 WP_349432301.1 sulfite exporter TauE/SafE family protein -
  Q9L42_RS06115 (Q9L42_006115) cysB 1249690..1250679 (+) 990 WP_349432303.1 HTH-type transcriptional regulator CysB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 41997.08 Da        Isoelectric Point: 5.9094

>NTDB_id=1008099 Q9L42_RS06095 WP_349432298.1 1245696..1246835(-) (pilU) [Methylomarinum sp. Ch1-1]
MDFNALLALMVQKKASDLFITAGKPPAMKVDGKVVEVSKTPLTAAQARSVVLGIMEQRQRDEFDNTKECQFALSTPKLGR
FRVSAFTQRDAAGMVLRRIESEIPEADDIHLPPVLKELIMHKRGLVIFVGATGTGKSTSLAALIRHRNENTSGHIITIED
PIEFEHPHLGCIITQREVGIDTESYEVALKNTLRQAPDVILIGEVRSRETMQHAITFAETGHLCLCTLHANNANQAIDRI
LHFFPDEMHGQLFMDLSLNLRGIVAQQLIKRADGNGRYPAIEILLNTPLVSDLIRKGEVHKLKELMKNSREHGMQTFDQA
LYDLYTAGKISYEDALNSADSRNEVRLMIKLGAENAGTLEGEGGMTLEETDEDAGGSLY

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1008099 Q9L42_RS06095 WP_349432298.1 1245696..1246835(-) (pilU) [Methylomarinum sp. Ch1-1]
ATGGATTTTAATGCGCTGCTGGCACTGATGGTGCAAAAAAAGGCCTCTGACCTGTTTATTACCGCTGGCAAACCGCCGGC
GATGAAAGTCGACGGCAAGGTCGTCGAAGTGTCTAAGACCCCGTTAACGGCGGCGCAAGCAAGGTCCGTGGTATTAGGCA
TCATGGAGCAACGACAACGGGATGAATTCGACAACACCAAGGAATGCCAATTTGCCCTGAGCACCCCTAAGCTCGGGCGC
TTCCGAGTCAGCGCCTTCACTCAACGGGATGCCGCCGGCATGGTGCTTAGACGGATCGAAAGCGAAATTCCCGAGGCCGA
TGACATTCATCTACCGCCAGTGCTGAAAGAACTGATCATGCATAAACGCGGCCTGGTGATTTTCGTCGGCGCAACCGGCA
CCGGTAAATCAACCTCATTGGCGGCCTTGATCCGCCATCGCAACGAAAACACCAGCGGCCATATCATCACCATCGAAGAC
CCGATCGAATTCGAACATCCACACCTGGGCTGCATTATCACCCAGCGAGAAGTCGGCATAGATACAGAATCTTATGAGGT
CGCCCTGAAAAACACGCTAAGGCAAGCGCCCGATGTCATCCTGATCGGCGAGGTGCGCTCTCGCGAAACCATGCAACACG
CAATCACCTTTGCCGAAACCGGTCATTTATGCCTATGCACACTGCATGCCAACAACGCCAACCAAGCCATAGACCGCATT
CTACACTTCTTCCCCGATGAAATGCATGGTCAGCTGTTCATGGATTTATCGCTGAACTTGCGCGGCATCGTCGCCCAACA
GCTGATCAAACGCGCCGACGGCAACGGCCGTTATCCGGCGATCGAAATACTGTTGAATACACCGCTGGTCTCCGACTTGA
TCCGCAAGGGCGAAGTCCACAAGCTGAAAGAGCTGATGAAAAACTCGCGCGAACACGGCATGCAAACCTTTGATCAGGCG
CTCTACGACCTCTACACCGCCGGCAAAATCAGCTATGAGGATGCATTGAATTCAGCCGACTCCAGAAACGAGGTCCGCTT
GATGATCAAGTTGGGCGCCGAAAATGCCGGCACGCTGGAAGGTGAAGGCGGCATGACCTTGGAAGAAACCGATGAAGACG
CCGGCGGCAGCTTGTATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(35-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

65.789

100

0.66
  pilU Acinetobacter baylyi ADP1

62.67

96.834

0.607
  pilU Vibrio cholerae strain A1552

56.571

92.348

0.522
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.816

91.821

0.393
  pilT Legionella pneumophila strain ERS1305867

42.857

90.501

0.388
  pilT Legionella pneumophila strain Lp02

42.857

90.501

0.388
  pilT Pseudomonas aeruginosa PAK

41.003

89.446

0.367
  pilT Acinetobacter nosocomialis M2

40.708

89.446

0.364
  pilT Acinetobacter baumannii D1279779

40.708

89.446

0.364
  pilT Acinetobacter baumannii strain A118

40.708

89.446

0.364

Multiple sequence alignment