Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABLV49_RS01425 Genome accession   NZ_CP157675
Coordinates   276686..277729 (-) Length   347 a.a.
NCBI ID   WP_011799709.1    Uniprot ID   A0AAU7LS57
Organism   Polaromonas hydrogenivorans strain DSM 17735     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 271686..282729
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLV49_RS01395 (ABLV49_01395) - 272003..272464 (+) 462 WP_349279884.1 YraN family protein -
  ABLV49_RS01400 (ABLV49_01400) - 272589..273188 (+) 600 WP_011799705.1 phosphoheptose isomerase -
  ABLV49_RS01405 (ABLV49_01405) - 273185..273850 (+) 666 WP_349279885.1 BON domain-containing protein -
  ABLV49_RS01410 (ABLV49_01410) - 273903..274805 (-) 903 WP_349279886.1 NAD(P)-dependent oxidoreductase -
  ABLV49_RS01415 (ABLV49_01415) pilU 274837..275973 (-) 1137 WP_349279887.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABLV49_RS01420 (ABLV49_01420) - 276021..276653 (-) 633 WP_349279888.1 cyclic nucleotide-binding domain-containing protein -
  ABLV49_RS01425 (ABLV49_01425) pilT 276686..277729 (-) 1044 WP_011799709.1 type IV pilus twitching motility protein PilT Machinery gene
  ABLV49_RS01430 (ABLV49_01430) - 277775..278506 (+) 732 WP_349279889.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABLV49_RS01435 (ABLV49_01435) - 278512..280974 (-) 2463 WP_349279890.1 EAL domain-containing protein -
  ABLV49_RS01440 (ABLV49_01440) wecB 280986..282176 (-) 1191 WP_349279891.1 UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing) -
  ABLV49_RS01445 (ABLV49_01445) - 282268..282528 (+) 261 WP_349279892.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38202.91 Da        Isoelectric Point: 6.7778

>NTDB_id=1008044 ABLV49_RS01425 WP_011799709.1 276686..277729(-) (pilT) [Polaromonas hydrogenivorans strain DSM 17735]
MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINIDPLDHKEVHAMVYDIMNDTQRKHYEEFLEVDFSFEIDGLAR
FRVNAFNHNRGAGAVLRTIPSKILTLEQLGCPKIFGDLALKPRGMVLVTGPTGSGKSTTLAAMVNYLNETEYGHILTVED
PIEFVHESKKCLINQREVGPHTLSFSAALKSALREDPDAILVGEMRDLETIRLAMTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAEEKEMIRSMLSESLQAVISQTLCKTKDGQGRVAAHEIMLGTSAIRNLIREAKVAQMYSSIQTGNSVGMQTLDQN
LTDLVKRNVISSAEARGKAKIPDNFPG

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=1008044 ABLV49_RS01425 WP_011799709.1 276686..277729(-) (pilT) [Polaromonas hydrogenivorans strain DSM 17735]
ATGGACATTACGCAGCTGCTGGCCTTTAGCGTCAAAAACAAGGCATCTGACTTGCATCTGTCGGCCGGCTTGCCGCCCAT
GATCCGGGTTCACGGCGACGTGCGGCGCATCAACATTGATCCGCTGGACCACAAGGAAGTGCATGCCATGGTGTACGACA
TCATGAACGACACCCAGCGCAAGCACTACGAAGAGTTCCTTGAGGTCGATTTTTCCTTCGAAATTGACGGTCTGGCGCGC
TTTCGCGTCAACGCCTTCAACCACAACCGGGGCGCTGGCGCGGTGCTGCGGACCATTCCGTCCAAAATCCTGACGCTGGA
GCAGCTCGGCTGCCCGAAGATTTTCGGCGACCTAGCCTTGAAGCCGCGCGGCATGGTACTGGTCACCGGCCCGACCGGCT
CGGGCAAATCGACCACGCTGGCGGCGATGGTCAACTACCTGAATGAAACCGAGTACGGCCACATCCTGACGGTCGAAGAC
CCAATCGAGTTTGTCCACGAATCCAAGAAATGCCTGATCAACCAGCGCGAGGTCGGCCCGCACACGCTGAGCTTCAGCGC
GGCGCTCAAGTCGGCCCTGCGCGAAGATCCGGATGCCATCCTGGTGGGCGAAATGCGCGACCTCGAAACCATTCGCCTGG
CGATGACGGCGGCTGAAACCGGCCACCTGGTGTTCGGCACGCTGCACACGTCGAGCGCCGCCAAGACCATCGACCGGATC
ATCGACGTGTTTCCGGCCGAGGAAAAGGAAATGATCCGCTCGATGCTGTCCGAATCGCTGCAGGCGGTGATCTCGCAAAC
CCTGTGCAAGACCAAGGACGGCCAGGGCCGGGTGGCGGCGCATGAAATCATGCTGGGCACCAGCGCCATCCGCAACCTGA
TCCGCGAAGCCAAGGTCGCGCAGATGTACTCGTCGATCCAGACCGGCAACAGCGTGGGCATGCAGACGCTGGACCAGAAC
CTGACGGACCTGGTCAAGCGCAACGTGATTTCCTCCGCGGAAGCACGCGGCAAAGCCAAGATTCCCGACAATTTCCCGGG
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

77.391

99.424

0.769
  pilT Pseudomonas stutzeri DSM 10701

76.522

99.424

0.761
  pilT Acinetobacter nosocomialis M2

75.942

99.424

0.755
  pilT Acinetobacter baumannii D1279779

75.942

99.424

0.755
  pilT Acinetobacter baumannii strain A118

75.942

99.424

0.755
  pilT Acinetobacter baylyi ADP1

75.072

99.424

0.746
  pilT Legionella pneumophila strain Lp02

73.746

97.695

0.72
  pilT Legionella pneumophila strain ERS1305867

73.746

97.695

0.72
  pilT Neisseria gonorrhoeae MS11

68.529

97.983

0.671
  pilT Neisseria meningitidis 8013

68.529

97.983

0.671
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.276

98.271

0.651
  pilT Vibrio cholerae strain A1552

66.276

98.271

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.304

99.424

0.51
  pilU Vibrio cholerae strain A1552

43.284

96.542

0.418
  pilU Acinetobacter baylyi ADP1

41.329

99.712

0.412
  pilU Pseudomonas stutzeri DSM 10701

41.791

96.542

0.403

Multiple sequence alignment